Immunometabolic crosstalk in Aedes fluviatilis and Wolbachia pipientis symbiosis

• Autor(a) principal: Silva, Jhenifer N.
• Data de Publicação: 2024
• Outros Autores: Calixto, Christiano, Brito, Gisely Cristina Ramos de, Daumas Filho, Carlos Renato de Oliveira, Silva, Ana Beatriz Walter Nuno da, Cunha, Octávio Augusto Talyuli da, Sampaio, Angélica Fernandes Arcanjo, Oliveira, Pedro Lagerblad de, Moreira, Luciano A., Vaz Junior, Itabajara da Silva, Logullo, Carlos
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/278456
• topic: Wolbachia; Glicogênio sintase quinase 3 beta; Transcrição genética; Metabolismo dos carboidratos; Respostas imunes; Células embrionárias; Aedes fluviatilis; Symbiosis; Immunometabolism; GSK3β; Relish2

Wolbachia pipientis is a maternally transmitted symbiotic bacterium that mainly colonizes arthropods, potentially affecting different aspects of the host’s physiology, e.g., reproduction, immunity, and metabolism. It has been shown that Wolbachia modulates glycogen metabolism in mosquito Aedes fluviatilis (Ae. fluviatilis). Glycogen synthesis is controlled by the enzyme GSK3, which is also involved in immune responses in both vertebrate and invertebrate organisms. Here we investigated the mechanisms behind immune changes mediated by glycogen synthase kinase β (GSK3β) in the symbiosis between Ae. fluviatilis and W. pipientis using a GSK3β inhibitor or RNAi-mediated gene silencing. GSK3β inhibition or knockdown increased glycogen content and Wolbachia population, together with a reduction in Relish2 and gambicin transcripts. Furthermore, knockdown of Relish2 or Caspar revealed that the immunodeficiency pathway acts to control Wolbachia numbers in the host. In conclusion, we describe for the first time the involvement of GSK3β in Ae. fluviatilis imune response, acting to control the Wolbachia endosymbiotic population.