Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/159265 |
Resumo: | Background Administering intravenous antibiotics during labor to women at risk for transmitting Group B Streptococcus (GBS) can prevent infections in newborns. However, the impact of intrapartum antibiotic prophylaxis on mothers' microbial community composition is largely unknown. We compared vaginal microbial composition in pregnant women experiencing preterm birth at 32 weeks gestation that received intrapartum antibiotic prophylaxis with that in controls. Methods Microbiota in vaginal swabs collected shortly before delivery from GBS positive women that received penicillin intravenously during labor or after premature rupture of membranes was compared to controls. Microbiota was analyzed by 16S rRNA sequencing using the PGM Ion Torrent to determine the effects of penicillin use during hospitalization and GBS status on its composition. Results Penicillin administration was associated with an altered vaginal microbial community composition characterized by increased microbial diversity. Lactobacillus sp. contributed only 13.1% of the total community in the women that received penicillin compared to 88.1% in the controls. Streptococcus sp. were present in higher abundance in GBS positive woman compared to controls, with 60% of the total vaginal microbiota in severe cases identified as Streptococcus sp. Conclusions Vaginal communities of healthy pregnant women were dominated by Lactobacillus sp. and contained low diversity, while Group B Streptococcus positive women receiving intrapartum antibiotic prophylaxis had a modified vaginal microbiota composition with low abundance of Lactobacillus but higher microbial diversity. |
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Roesch, Luiz Fernando WurdigSilveira, Rita de Cássia dos SantosCorso, Andréa LúciaDobbler, Priscila Caroline ThiagoMai, VolkerRojas, Bruna SchaferLaureano, Álvaro MacedoProcianoy, Renato Soibelmann2017-06-07T02:40:48Z20171932-6203http://hdl.handle.net/10183/159265001013684Background Administering intravenous antibiotics during labor to women at risk for transmitting Group B Streptococcus (GBS) can prevent infections in newborns. However, the impact of intrapartum antibiotic prophylaxis on mothers' microbial community composition is largely unknown. We compared vaginal microbial composition in pregnant women experiencing preterm birth at 32 weeks gestation that received intrapartum antibiotic prophylaxis with that in controls. Methods Microbiota in vaginal swabs collected shortly before delivery from GBS positive women that received penicillin intravenously during labor or after premature rupture of membranes was compared to controls. Microbiota was analyzed by 16S rRNA sequencing using the PGM Ion Torrent to determine the effects of penicillin use during hospitalization and GBS status on its composition. Results Penicillin administration was associated with an altered vaginal microbial community composition characterized by increased microbial diversity. Lactobacillus sp. contributed only 13.1% of the total community in the women that received penicillin compared to 88.1% in the controls. Streptococcus sp. were present in higher abundance in GBS positive woman compared to controls, with 60% of the total vaginal microbiota in severe cases identified as Streptococcus sp. Conclusions Vaginal communities of healthy pregnant women were dominated by Lactobacillus sp. and contained low diversity, while Group B Streptococcus positive women receiving intrapartum antibiotic prophylaxis had a modified vaginal microbiota composition with low abundance of Lactobacillus but higher microbial diversity.application/pdfengPLoS ONE. San Francisco. Vol. 12, no. 2 (Feb. 2017), e0169916, 13 p.GestantesDoenças vaginaisMicrobiotaInfecções estreptocócicasPenicilinasDiversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillinEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001013684.pdf001013684.pdfTexto completo (inglês)application/pdf1952446http://www.lume.ufrgs.br/bitstream/10183/159265/1/001013684.pdf2c4d5adbbcc069d0b662455768a90fceMD51TEXT001013684.pdf.txt001013684.pdf.txtExtracted Texttext/plain41203http://www.lume.ufrgs.br/bitstream/10183/159265/2/001013684.pdf.txtc5d556c0c57f7ea6dbdd38c3e6e6819dMD52THUMBNAIL001013684.pdf.jpg001013684.pdf.jpgGenerated Thumbnailimage/jpeg1980http://www.lume.ufrgs.br/bitstream/10183/159265/3/001013684.pdf.jpg6e42e880704ee0bb8d966696714ca28fMD5310183/1592652023-04-30 03:58:02.358458oai:www.lume.ufrgs.br:10183/159265Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-04-30T06:58:02Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin |
title |
Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin |
spellingShingle |
Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin Roesch, Luiz Fernando Wurdig Gestantes Doenças vaginais Microbiota Infecções estreptocócicas Penicilinas |
title_short |
Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin |
title_full |
Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin |
title_fullStr |
Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin |
title_full_unstemmed |
Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin |
title_sort |
Diversity and composition of vaginal microbiota of pregnant women at risk for transmitting Group B Streptococcus treated with intrapartum penicillin |
author |
Roesch, Luiz Fernando Wurdig |
author_facet |
Roesch, Luiz Fernando Wurdig Silveira, Rita de Cássia dos Santos Corso, Andréa Lúcia Dobbler, Priscila Caroline Thiago Mai, Volker Rojas, Bruna Schafer Laureano, Álvaro Macedo Procianoy, Renato Soibelmann |
author_role |
author |
author2 |
Silveira, Rita de Cássia dos Santos Corso, Andréa Lúcia Dobbler, Priscila Caroline Thiago Mai, Volker Rojas, Bruna Schafer Laureano, Álvaro Macedo Procianoy, Renato Soibelmann |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Roesch, Luiz Fernando Wurdig Silveira, Rita de Cássia dos Santos Corso, Andréa Lúcia Dobbler, Priscila Caroline Thiago Mai, Volker Rojas, Bruna Schafer Laureano, Álvaro Macedo Procianoy, Renato Soibelmann |
dc.subject.por.fl_str_mv |
Gestantes Doenças vaginais Microbiota Infecções estreptocócicas Penicilinas |
topic |
Gestantes Doenças vaginais Microbiota Infecções estreptocócicas Penicilinas |
description |
Background Administering intravenous antibiotics during labor to women at risk for transmitting Group B Streptococcus (GBS) can prevent infections in newborns. However, the impact of intrapartum antibiotic prophylaxis on mothers' microbial community composition is largely unknown. We compared vaginal microbial composition in pregnant women experiencing preterm birth at 32 weeks gestation that received intrapartum antibiotic prophylaxis with that in controls. Methods Microbiota in vaginal swabs collected shortly before delivery from GBS positive women that received penicillin intravenously during labor or after premature rupture of membranes was compared to controls. Microbiota was analyzed by 16S rRNA sequencing using the PGM Ion Torrent to determine the effects of penicillin use during hospitalization and GBS status on its composition. Results Penicillin administration was associated with an altered vaginal microbial community composition characterized by increased microbial diversity. Lactobacillus sp. contributed only 13.1% of the total community in the women that received penicillin compared to 88.1% in the controls. Streptococcus sp. were present in higher abundance in GBS positive woman compared to controls, with 60% of the total vaginal microbiota in severe cases identified as Streptococcus sp. Conclusions Vaginal communities of healthy pregnant women were dominated by Lactobacillus sp. and contained low diversity, while Group B Streptococcus positive women receiving intrapartum antibiotic prophylaxis had a modified vaginal microbiota composition with low abundance of Lactobacillus but higher microbial diversity. |
publishDate |
2017 |
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2017-06-07T02:40:48Z |
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2017 |
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1932-6203 |
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001013684 |
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eng |
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PLoS ONE. San Francisco. Vol. 12, no. 2 (Feb. 2017), e0169916, 13 p. |
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openAccess |
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