Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225440 |
Resumo: | Fungal chitin metabolism involves diverse processes such as metabolically active cell wall maintenance, basic nutrition, and different aspects of virulence. Chitinases are enzymes belonging to the glycoside hydrolase family 18 (GH18) and 19 (GH19) and are responsible for the hydrolysis of b-1,4-linkages in chitin. This linear homopolymer of N-acetyl-b-D-glucosamine is an essential constituent of fungal cell walls and arthropod exoskeletons. Several chitinases have been directly implicated in structural, morphogenetic, autolytic and nutritional activities of fungal cells. In the entomopathogen Metarhizium anisopliae, chitinases are also involved in virulence. Filamentous fungi genomes exhibit a higher number of chitinase-coding genes than bacteria or yeasts. The survey performed in the M. anisopliae genome has successfully identified 24 genes belonging to glycoside hydrolase family 18, including three previously experimentally determined chitinase-coding genes named chit1, chi2 and chi3. These putative chitinases were classified based on domain organization and phylogenetic analysis into the previously described A, B and C chitinase subgroups, and into a new subgroup D. Moreover, three GH18 proteins could be classified as putative endo-N-acetyl-b-D-glucosaminidases, enzymes that are associated with deglycosylation and were therefore assigned to a new subgroup E. The transcriptional profile of the GH18 genes was evaluated by qPCR with RNA extracted from eight culture conditions, representing different stages of development or different nutritional states. The transcripts from the GH18 genes were detected in at least one of the different M. anisopliae developmental stages, thus validating the proposed genes. Moreover, not all members from the same chitinase subgroup presented equal patterns of transcript expression under the eight distinct conditions studied. The determination of M. anisopliae chitinases and ENGases and a more detailed study concerning the enzymes’ roles in morphological or nutritional functions will allow comprehensive insights into the chitinolytic potential of this highly infective entomopathogenic fungus. |
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Junges, AngelaBoldo, Juliano TomazzoniSouza, Bárbara KunzlerGuedes, Rafael Lucas MunizOliveira, Nicolau SbarainiSilva, Lívia Kmetzsch Rosa eThompson, Claudia ElizabethStaats, Charley ChristianAlmeida, Luis Gonzaga de PaulaVasconcelos, Ana Tereza Ribeiro deVainstein, Marilene HenningSchrank, Augusto2021-08-10T04:31:56Z20141932-6203http://hdl.handle.net/10183/225440000994761Fungal chitin metabolism involves diverse processes such as metabolically active cell wall maintenance, basic nutrition, and different aspects of virulence. Chitinases are enzymes belonging to the glycoside hydrolase family 18 (GH18) and 19 (GH19) and are responsible for the hydrolysis of b-1,4-linkages in chitin. This linear homopolymer of N-acetyl-b-D-glucosamine is an essential constituent of fungal cell walls and arthropod exoskeletons. Several chitinases have been directly implicated in structural, morphogenetic, autolytic and nutritional activities of fungal cells. In the entomopathogen Metarhizium anisopliae, chitinases are also involved in virulence. Filamentous fungi genomes exhibit a higher number of chitinase-coding genes than bacteria or yeasts. The survey performed in the M. anisopliae genome has successfully identified 24 genes belonging to glycoside hydrolase family 18, including three previously experimentally determined chitinase-coding genes named chit1, chi2 and chi3. These putative chitinases were classified based on domain organization and phylogenetic analysis into the previously described A, B and C chitinase subgroups, and into a new subgroup D. Moreover, three GH18 proteins could be classified as putative endo-N-acetyl-b-D-glucosaminidases, enzymes that are associated with deglycosylation and were therefore assigned to a new subgroup E. The transcriptional profile of the GH18 genes was evaluated by qPCR with RNA extracted from eight culture conditions, representing different stages of development or different nutritional states. The transcripts from the GH18 genes were detected in at least one of the different M. anisopliae developmental stages, thus validating the proposed genes. Moreover, not all members from the same chitinase subgroup presented equal patterns of transcript expression under the eight distinct conditions studied. The determination of M. anisopliae chitinases and ENGases and a more detailed study concerning the enzymes’ roles in morphological or nutritional functions will allow comprehensive insights into the chitinolytic potential of this highly infective entomopathogenic fungus.application/pdfengPLoS ONE. San Francisco. Vol. 9, no. 9 (Sept. 2014), e107864, 16 p.Metarhizium anisopliae : Fungo entomopatogenicoHidrolasesGenomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliaeEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000994761.pdf.txt000994761.pdf.txtExtracted Texttext/plain83943http://www.lume.ufrgs.br/bitstream/10183/225440/2/000994761.pdf.txtb29b73fea537c8e654c2c3f57e6abcb1MD52ORIGINAL000994761.pdfTexto completo (inglês)application/pdf4237469http://www.lume.ufrgs.br/bitstream/10183/225440/1/000994761.pdf81e36cb1b24aabe615efb128850346baMD5110183/2254402023-09-24 03:39:14.831961oai:www.lume.ufrgs.br:10183/225440Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-09-24T06:39:14Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae |
title |
Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae |
spellingShingle |
Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae Junges, Angela Metarhizium anisopliae : Fungo entomopatogenico Hidrolases |
title_short |
Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae |
title_full |
Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae |
title_fullStr |
Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae |
title_full_unstemmed |
Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae |
title_sort |
Genomic analyses and transcriptional profiles of the glycoside hydrolase family 18 genes of the entomopathogenic fungus Metarhizium anisopliae |
author |
Junges, Angela |
author_facet |
Junges, Angela Boldo, Juliano Tomazzoni Souza, Bárbara Kunzler Guedes, Rafael Lucas Muniz Oliveira, Nicolau Sbaraini Silva, Lívia Kmetzsch Rosa e Thompson, Claudia Elizabeth Staats, Charley Christian Almeida, Luis Gonzaga de Paula Vasconcelos, Ana Tereza Ribeiro de Vainstein, Marilene Henning Schrank, Augusto |
author_role |
author |
author2 |
Boldo, Juliano Tomazzoni Souza, Bárbara Kunzler Guedes, Rafael Lucas Muniz Oliveira, Nicolau Sbaraini Silva, Lívia Kmetzsch Rosa e Thompson, Claudia Elizabeth Staats, Charley Christian Almeida, Luis Gonzaga de Paula Vasconcelos, Ana Tereza Ribeiro de Vainstein, Marilene Henning Schrank, Augusto |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Junges, Angela Boldo, Juliano Tomazzoni Souza, Bárbara Kunzler Guedes, Rafael Lucas Muniz Oliveira, Nicolau Sbaraini Silva, Lívia Kmetzsch Rosa e Thompson, Claudia Elizabeth Staats, Charley Christian Almeida, Luis Gonzaga de Paula Vasconcelos, Ana Tereza Ribeiro de Vainstein, Marilene Henning Schrank, Augusto |
dc.subject.por.fl_str_mv |
Metarhizium anisopliae : Fungo entomopatogenico Hidrolases |
topic |
Metarhizium anisopliae : Fungo entomopatogenico Hidrolases |
description |
Fungal chitin metabolism involves diverse processes such as metabolically active cell wall maintenance, basic nutrition, and different aspects of virulence. Chitinases are enzymes belonging to the glycoside hydrolase family 18 (GH18) and 19 (GH19) and are responsible for the hydrolysis of b-1,4-linkages in chitin. This linear homopolymer of N-acetyl-b-D-glucosamine is an essential constituent of fungal cell walls and arthropod exoskeletons. Several chitinases have been directly implicated in structural, morphogenetic, autolytic and nutritional activities of fungal cells. In the entomopathogen Metarhizium anisopliae, chitinases are also involved in virulence. Filamentous fungi genomes exhibit a higher number of chitinase-coding genes than bacteria or yeasts. The survey performed in the M. anisopliae genome has successfully identified 24 genes belonging to glycoside hydrolase family 18, including three previously experimentally determined chitinase-coding genes named chit1, chi2 and chi3. These putative chitinases were classified based on domain organization and phylogenetic analysis into the previously described A, B and C chitinase subgroups, and into a new subgroup D. Moreover, three GH18 proteins could be classified as putative endo-N-acetyl-b-D-glucosaminidases, enzymes that are associated with deglycosylation and were therefore assigned to a new subgroup E. The transcriptional profile of the GH18 genes was evaluated by qPCR with RNA extracted from eight culture conditions, representing different stages of development or different nutritional states. The transcripts from the GH18 genes were detected in at least one of the different M. anisopliae developmental stages, thus validating the proposed genes. Moreover, not all members from the same chitinase subgroup presented equal patterns of transcript expression under the eight distinct conditions studied. The determination of M. anisopliae chitinases and ENGases and a more detailed study concerning the enzymes’ roles in morphological or nutritional functions will allow comprehensive insights into the chitinolytic potential of this highly infective entomopathogenic fungus. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2021-08-10T04:31:56Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/225440 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000994761 |
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1932-6203 000994761 |
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http://hdl.handle.net/10183/225440 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE. San Francisco. Vol. 9, no. 9 (Sept. 2014), e107864, 16 p. |
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