Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/253607 |
Resumo: | Medical devices (indwelling) have greatly improved healthcare. Nevertheless, infections related to the use of these apparatuses continue to be a major clinical concern. Biofilms form on surfaces after bacterial adhesion, and they function as bacterial reservoirs and as resistance and tolerance factors against antibiotics and the host immune response. Technological strategies to control biofilms and bacterial adhesion, such as the use of surface coatings, are being explored more frequently, and natural peptides may promote their development. In this study, we purified and identified antibiofilm peptides from Capsicum baccatum (red pepper) using chromatography- tandem mass spectrometry, MALDI-MS, MS/MS and bioinformatics. These peptides strongly controlled biofilm formation by Staphylococcus epidermidis, the most prevalent pathogen in device-related infections, without any antibiotic activity. Furthermore, natural peptide-coated surfaces dislayed effective antiadhesive proprieties and showed no cytotoxic effects against different representative human cell lines. Finally, we determined the lead peptide predicted by Mascot and identified CSP37, which may be useful as a prime structure for the design of new antibiofilm agents. Together, these results shed light on natural Capsicum peptides as a possible antiadhesive coat to prevent medical device colonization. |
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Von Borowski, Rafael GomesBarros, Muriel Primon deSilva, Denise BrentanLopes, Norberto PeporineZimmer, Karine RigonStaats, Charley ChristianOliveira, Cristiane Bernardes deGiudice, EmmanuelGillet, ReynaldMacedo, Alexandre JoséGnoatto, Simone Cristina BaggioZimmer, Aline Rigon2023-01-12T04:56:31Z20200378-5173http://hdl.handle.net/10183/253607001158310Medical devices (indwelling) have greatly improved healthcare. Nevertheless, infections related to the use of these apparatuses continue to be a major clinical concern. Biofilms form on surfaces after bacterial adhesion, and they function as bacterial reservoirs and as resistance and tolerance factors against antibiotics and the host immune response. Technological strategies to control biofilms and bacterial adhesion, such as the use of surface coatings, are being explored more frequently, and natural peptides may promote their development. In this study, we purified and identified antibiofilm peptides from Capsicum baccatum (red pepper) using chromatography- tandem mass spectrometry, MALDI-MS, MS/MS and bioinformatics. These peptides strongly controlled biofilm formation by Staphylococcus epidermidis, the most prevalent pathogen in device-related infections, without any antibiotic activity. Furthermore, natural peptide-coated surfaces dislayed effective antiadhesive proprieties and showed no cytotoxic effects against different representative human cell lines. Finally, we determined the lead peptide predicted by Mascot and identified CSP37, which may be useful as a prime structure for the design of new antibiofilm agents. Together, these results shed light on natural Capsicum peptides as a possible antiadhesive coat to prevent medical device colonization.application/pdfengInternational journal of pharmaceutics. Amsterdam. Vol. 574 (Jan. 2020), 118872, 11 p.BiofilmesPeptídeosBiofilmAntibiofilmAntiadhesiveCapsicumPeptideCoatingRed pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formationEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001158310.pdf.txt001158310.pdf.txtExtracted Texttext/plain59421http://www.lume.ufrgs.br/bitstream/10183/253607/2/001158310.pdf.txt2ee35f53b19343a80379cf58c62e941dMD52ORIGINAL001158310.pdfTexto completo (inglês)application/pdf2127932http://www.lume.ufrgs.br/bitstream/10183/253607/1/001158310.pdfb6c852930a07a09d9994c2a86d1844f8MD5110183/2536072023-06-21 03:31:12.495291oai:www.lume.ufrgs.br:10183/253607Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-06-21T06:31:12Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation |
title |
Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation |
spellingShingle |
Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation Von Borowski, Rafael Gomes Biofilmes Peptídeos Biofilm Antibiofilm Antiadhesive Capsicum Peptide Coating |
title_short |
Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation |
title_full |
Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation |
title_fullStr |
Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation |
title_full_unstemmed |
Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation |
title_sort |
Red pepper peptide coatings control Staphylococcus epidermidis adhesion and biofilm formation |
author |
Von Borowski, Rafael Gomes |
author_facet |
Von Borowski, Rafael Gomes Barros, Muriel Primon de Silva, Denise Brentan Lopes, Norberto Peporine Zimmer, Karine Rigon Staats, Charley Christian Oliveira, Cristiane Bernardes de Giudice, Emmanuel Gillet, Reynald Macedo, Alexandre José Gnoatto, Simone Cristina Baggio Zimmer, Aline Rigon |
author_role |
author |
author2 |
Barros, Muriel Primon de Silva, Denise Brentan Lopes, Norberto Peporine Zimmer, Karine Rigon Staats, Charley Christian Oliveira, Cristiane Bernardes de Giudice, Emmanuel Gillet, Reynald Macedo, Alexandre José Gnoatto, Simone Cristina Baggio Zimmer, Aline Rigon |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Von Borowski, Rafael Gomes Barros, Muriel Primon de Silva, Denise Brentan Lopes, Norberto Peporine Zimmer, Karine Rigon Staats, Charley Christian Oliveira, Cristiane Bernardes de Giudice, Emmanuel Gillet, Reynald Macedo, Alexandre José Gnoatto, Simone Cristina Baggio Zimmer, Aline Rigon |
dc.subject.por.fl_str_mv |
Biofilmes Peptídeos |
topic |
Biofilmes Peptídeos Biofilm Antibiofilm Antiadhesive Capsicum Peptide Coating |
dc.subject.eng.fl_str_mv |
Biofilm Antibiofilm Antiadhesive Capsicum Peptide Coating |
description |
Medical devices (indwelling) have greatly improved healthcare. Nevertheless, infections related to the use of these apparatuses continue to be a major clinical concern. Biofilms form on surfaces after bacterial adhesion, and they function as bacterial reservoirs and as resistance and tolerance factors against antibiotics and the host immune response. Technological strategies to control biofilms and bacterial adhesion, such as the use of surface coatings, are being explored more frequently, and natural peptides may promote their development. In this study, we purified and identified antibiofilm peptides from Capsicum baccatum (red pepper) using chromatography- tandem mass spectrometry, MALDI-MS, MS/MS and bioinformatics. These peptides strongly controlled biofilm formation by Staphylococcus epidermidis, the most prevalent pathogen in device-related infections, without any antibiotic activity. Furthermore, natural peptide-coated surfaces dislayed effective antiadhesive proprieties and showed no cytotoxic effects against different representative human cell lines. Finally, we determined the lead peptide predicted by Mascot and identified CSP37, which may be useful as a prime structure for the design of new antibiofilm agents. Together, these results shed light on natural Capsicum peptides as a possible antiadhesive coat to prevent medical device colonization. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2023-01-12T04:56:31Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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http://hdl.handle.net/10183/253607 |
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0378-5173 |
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001158310 |
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http://hdl.handle.net/10183/253607 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
International journal of pharmaceutics. Amsterdam. Vol. 574 (Jan. 2020), 118872, 11 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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