Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/233003 |
Resumo: | Bovine viral diarrhea virus (BVDV) comprises two species, BVDV-1 and BVDV-2. But given the genetic diversity among pestiviruses, at least 22 subgenotypes are described for BVDV-1 and 3-4 for BVDV-2. Genetic characterization is generally accomplished through complete or partial sequencing and phylogeny, but it is not a reliable method to define antigenic relationships. The traditional method for evaluating antigenic relationships between pestivirus isolates is the virus neutralization (VN) assay, but interpretation of the data to define antigenic relatedness can be difficult to discern for BVDV isolates within the same BVDV species. Data from this study utilized a multivariate analysis for visualization of VN results to analyze the antigenic relationships between US vaccine strains and field isolates from Switzerland, Italy, Brazil, and the UK. Polyclonal sera were generated against six BVDV strains currently contained in vaccine formulations, and each serum was used in VNs to measure the titers against seven vaccine strains (including the six homologous strains) and 23 BVDV field isolates. Principal component analysis (PCA) was performed using VN titers, and results were interpreted from PCA clustering within the PCA dendrogram and scatter plot. The results demonstrated clustering patterns among various isolates suggesting antigenic relatedness. As expected, the BVDV-1 and BVDV-2 isolates did not cluster together and had the greatest spatial distribution. Notably, a number of clusters representing antigenically related BVDV-1 subgroups contain isolates of different subgenotypes. The multivariate analysis may be a method to better characterize antigenic relationships among BVDV isolates that belong to the same BVDV species and do not have distinct antigenic differences. This might be an invaluable tool to ameliorate the composition of current vaccines, which might well be important for the success of any BVDV control program that includes vaccination in its scheme. |
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Mósena, Ana Cristina SbarainiFalkenberg, Shollie M.Ma, HaoCasas, EduardoDassanayake, Rohana P.Booth, RichardDe Mia, Gian MarioSchweizer, MatthiasCanal, Cláudio WageckNeill, John D.2021-12-15T04:26:50Z20220166-0934http://hdl.handle.net/10183/233003001133969Bovine viral diarrhea virus (BVDV) comprises two species, BVDV-1 and BVDV-2. But given the genetic diversity among pestiviruses, at least 22 subgenotypes are described for BVDV-1 and 3-4 for BVDV-2. Genetic characterization is generally accomplished through complete or partial sequencing and phylogeny, but it is not a reliable method to define antigenic relationships. The traditional method for evaluating antigenic relationships between pestivirus isolates is the virus neutralization (VN) assay, but interpretation of the data to define antigenic relatedness can be difficult to discern for BVDV isolates within the same BVDV species. Data from this study utilized a multivariate analysis for visualization of VN results to analyze the antigenic relationships between US vaccine strains and field isolates from Switzerland, Italy, Brazil, and the UK. Polyclonal sera were generated against six BVDV strains currently contained in vaccine formulations, and each serum was used in VNs to measure the titers against seven vaccine strains (including the six homologous strains) and 23 BVDV field isolates. Principal component analysis (PCA) was performed using VN titers, and results were interpreted from PCA clustering within the PCA dendrogram and scatter plot. The results demonstrated clustering patterns among various isolates suggesting antigenic relatedness. As expected, the BVDV-1 and BVDV-2 isolates did not cluster together and had the greatest spatial distribution. Notably, a number of clusters representing antigenically related BVDV-1 subgroups contain isolates of different subgenotypes. The multivariate analysis may be a method to better characterize antigenic relationships among BVDV isolates that belong to the same BVDV species and do not have distinct antigenic differences. This might be an invaluable tool to ameliorate the composition of current vaccines, which might well be important for the success of any BVDV control program that includes vaccination in its scheme.application/pdfengJournal of virological methods. Amsterdam. Vol. 299 (Jan. 2022), 114328, 10 p.Análise de componente principalAnálise multivariadaAntígenos viraisVírus da diarréia viral bovina tipo 1Vírus da diarréia viral bovina tipo 2Testes de neutralizaçãoVacinasPrincipal component analysisAntigenic characterizationBovine viral diarrhea virusVirus neutralizationCross neutralizationUse of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolatesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001133969.pdf.txt001133969.pdf.txtExtracted Texttext/plain56876http://www.lume.ufrgs.br/bitstream/10183/233003/2/001133969.pdf.txtd7b4a0766e9fd75c77626b95dfea0f93MD52ORIGINAL001133969.pdfTexto completo (inglês)application/pdf2584601http://www.lume.ufrgs.br/bitstream/10183/233003/1/001133969.pdfd22c1418d29fadc8c22610a2b4e8d0bbMD5110183/2330032022-01-07 05:33:32.856081oai:www.lume.ufrgs.br:10183/233003Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-01-07T07:33:32Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates |
title |
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates |
spellingShingle |
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates Mósena, Ana Cristina Sbaraini Análise de componente principal Análise multivariada Antígenos virais Vírus da diarréia viral bovina tipo 1 Vírus da diarréia viral bovina tipo 2 Testes de neutralização Vacinas Principal component analysis Antigenic characterization Bovine viral diarrhea virus Virus neutralization Cross neutralization |
title_short |
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates |
title_full |
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates |
title_fullStr |
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates |
title_full_unstemmed |
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates |
title_sort |
Use of multivariate analysis to evaluate antigenic relationships between US BVDV vaccine strains and non-US genetically divergent isolates |
author |
Mósena, Ana Cristina Sbaraini |
author_facet |
Mósena, Ana Cristina Sbaraini Falkenberg, Shollie M. Ma, Hao Casas, Eduardo Dassanayake, Rohana P. Booth, Richard De Mia, Gian Mario Schweizer, Matthias Canal, Cláudio Wageck Neill, John D. |
author_role |
author |
author2 |
Falkenberg, Shollie M. Ma, Hao Casas, Eduardo Dassanayake, Rohana P. Booth, Richard De Mia, Gian Mario Schweizer, Matthias Canal, Cláudio Wageck Neill, John D. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Mósena, Ana Cristina Sbaraini Falkenberg, Shollie M. Ma, Hao Casas, Eduardo Dassanayake, Rohana P. Booth, Richard De Mia, Gian Mario Schweizer, Matthias Canal, Cláudio Wageck Neill, John D. |
dc.subject.por.fl_str_mv |
Análise de componente principal Análise multivariada Antígenos virais Vírus da diarréia viral bovina tipo 1 Vírus da diarréia viral bovina tipo 2 Testes de neutralização Vacinas |
topic |
Análise de componente principal Análise multivariada Antígenos virais Vírus da diarréia viral bovina tipo 1 Vírus da diarréia viral bovina tipo 2 Testes de neutralização Vacinas Principal component analysis Antigenic characterization Bovine viral diarrhea virus Virus neutralization Cross neutralization |
dc.subject.eng.fl_str_mv |
Principal component analysis Antigenic characterization Bovine viral diarrhea virus Virus neutralization Cross neutralization |
description |
Bovine viral diarrhea virus (BVDV) comprises two species, BVDV-1 and BVDV-2. But given the genetic diversity among pestiviruses, at least 22 subgenotypes are described for BVDV-1 and 3-4 for BVDV-2. Genetic characterization is generally accomplished through complete or partial sequencing and phylogeny, but it is not a reliable method to define antigenic relationships. The traditional method for evaluating antigenic relationships between pestivirus isolates is the virus neutralization (VN) assay, but interpretation of the data to define antigenic relatedness can be difficult to discern for BVDV isolates within the same BVDV species. Data from this study utilized a multivariate analysis for visualization of VN results to analyze the antigenic relationships between US vaccine strains and field isolates from Switzerland, Italy, Brazil, and the UK. Polyclonal sera were generated against six BVDV strains currently contained in vaccine formulations, and each serum was used in VNs to measure the titers against seven vaccine strains (including the six homologous strains) and 23 BVDV field isolates. Principal component analysis (PCA) was performed using VN titers, and results were interpreted from PCA clustering within the PCA dendrogram and scatter plot. The results demonstrated clustering patterns among various isolates suggesting antigenic relatedness. As expected, the BVDV-1 and BVDV-2 isolates did not cluster together and had the greatest spatial distribution. Notably, a number of clusters representing antigenically related BVDV-1 subgroups contain isolates of different subgenotypes. The multivariate analysis may be a method to better characterize antigenic relationships among BVDV isolates that belong to the same BVDV species and do not have distinct antigenic differences. This might be an invaluable tool to ameliorate the composition of current vaccines, which might well be important for the success of any BVDV control program that includes vaccination in its scheme. |
publishDate |
2021 |
dc.date.accessioned.fl_str_mv |
2021-12-15T04:26:50Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/233003 |
dc.identifier.issn.pt_BR.fl_str_mv |
0166-0934 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001133969 |
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0166-0934 001133969 |
url |
http://hdl.handle.net/10183/233003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of virological methods. Amsterdam. Vol. 299 (Jan. 2022), 114328, 10 p. |
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openAccess |
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