Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/187209 |
Resumo: | The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, andmany other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points. |
id |
UFRGS-2_7e1b3bf9e76e3d20c21fe4c9637aa561 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/187209 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Théry, ClotildeZuba-Surma, Ewa K.Veit, Tiago Degani2018-12-19T04:07:18Z20182001-3078http://hdl.handle.net/10183/187209001082698The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, andmany other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.application/pdfengJournal of Extracellular Vesicles. Järfälla, Sweden. Vol. 8, no.1, (2018), 1535750Vesículas extracelularesExossomosMicropartículas derivadas de célulasExtracellular vesiclesExosomesEctosomesMicrovesiclesMinimal information requirementsGuidelinesStandardizationMicroparticlesRigorReproducibilityMinimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelinesEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001082698.pdf.txt001082698.pdf.txtExtracted Texttext/plain263761http://www.lume.ufrgs.br/bitstream/10183/187209/2/001082698.pdf.txt84e917369d774b8e93ce108184d9ea3bMD52ORIGINAL001082698.pdfTexto completo (inglês)application/pdf3666561http://www.lume.ufrgs.br/bitstream/10183/187209/1/001082698.pdfcb2b74f9d2ffa192579fc185a20f9ec8MD5110183/1872092018-12-20 04:03:35.066582oai:www.lume.ufrgs.br:10183/187209Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-12-20T06:03:35Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines |
title |
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines |
spellingShingle |
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines Théry, Clotilde Vesículas extracelulares Exossomos Micropartículas derivadas de células Extracellular vesicles Exosomes Ectosomes Microvesicles Minimal information requirements Guidelines Standardization Microparticles Rigor Reproducibility |
title_short |
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines |
title_full |
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines |
title_fullStr |
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines |
title_full_unstemmed |
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines |
title_sort |
Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines |
author |
Théry, Clotilde |
author_facet |
Théry, Clotilde Zuba-Surma, Ewa K. Veit, Tiago Degani |
author_role |
author |
author2 |
Zuba-Surma, Ewa K. Veit, Tiago Degani |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Théry, Clotilde Zuba-Surma, Ewa K. Veit, Tiago Degani |
dc.subject.por.fl_str_mv |
Vesículas extracelulares Exossomos Micropartículas derivadas de células |
topic |
Vesículas extracelulares Exossomos Micropartículas derivadas de células Extracellular vesicles Exosomes Ectosomes Microvesicles Minimal information requirements Guidelines Standardization Microparticles Rigor Reproducibility |
dc.subject.eng.fl_str_mv |
Extracellular vesicles Exosomes Ectosomes Microvesicles Minimal information requirements Guidelines Standardization Microparticles Rigor Reproducibility |
description |
The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, andmany other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles (“MISEV”) guidelines for the field in 2014. We now update these “MISEV2014” guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-12-19T04:07:18Z |
dc.date.issued.fl_str_mv |
2018 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/187209 |
dc.identifier.issn.pt_BR.fl_str_mv |
2001-3078 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001082698 |
identifier_str_mv |
2001-3078 001082698 |
url |
http://hdl.handle.net/10183/187209 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of Extracellular Vesicles. Järfälla, Sweden. Vol. 8, no.1, (2018), 1535750 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/187209/2/001082698.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/187209/1/001082698.pdf |
bitstream.checksum.fl_str_mv |
84e917369d774b8e93ce108184d9ea3b cb2b74f9d2ffa192579fc185a20f9ec8 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801224958574067712 |