Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P

Detalhes bibliográficos
Autor(a) principal: Mertins, Omar
Data de Publicação: 2008
Outros Autores: Sebben, Marcelo, Schneider, Paulo Henrique, Pohlmann, Adriana Raffin, Silveira, Nádya Pesce da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/72870
Resumo: A strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of 1H NMR spectra. Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm. Before purification, a ratio of 19.68¡ ±1.37 was determined. Using extraction, a ratio of 10.70¡ ±0.61 was found, while from column chromatography, a value of 2.99¡ ±0.25 was detected. 31P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.
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spelling Mertins, OmarSebben, MarceloSchneider, Paulo HenriquePohlmann, Adriana RaffinSilveira, Nádya Pesce da2013-07-02T01:43:15Z20080100-4042http://hdl.handle.net/10183/72870000668477A strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of 1H NMR spectra. Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm. Before purification, a ratio of 19.68¡ ±1.37 was determined. Using extraction, a ratio of 10.70¡ ±0.61 was found, while from column chromatography, a value of 2.99¡ ±0.25 was detected. 31P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.application/pdfporQuímica nova. São Paulo. Vol. 31, n. 7 (2008), p. 1856-1859Ressonância magnética nuclearLecitina de sojaNMRPhosphatidylcholinePurity determinationCaracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31PCharacterization of soybean phosphatidylcholine purity by 1h and 31p nmr info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000668477.pdf000668477.pdfTexto completoapplication/pdf179374http://www.lume.ufrgs.br/bitstream/10183/72870/1/000668477.pdf3f60e91b9aaa649e752d8b375dbb310bMD51TEXT000668477.pdf.txt000668477.pdf.txtExtracted Texttext/plain17452http://www.lume.ufrgs.br/bitstream/10183/72870/2/000668477.pdf.txt9e55f8121eb7f9d2f38913e181971789MD52THUMBNAIL000668477.pdf.jpg000668477.pdf.jpgGenerated Thumbnailimage/jpeg1970http://www.lume.ufrgs.br/bitstream/10183/72870/3/000668477.pdf.jpgdb11d327746525b723b5feae1bd16176MD5310183/728702018-10-15 08:10:42.302oai:www.lume.ufrgs.br:10183/72870Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-15T11:10:42Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P
dc.title.alternative.en.fl_str_mv Characterization of soybean phosphatidylcholine purity by 1h and 31p nmr
title Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P
spellingShingle Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P
Mertins, Omar
Ressonância magnética nuclear
Lecitina de soja
NMR
Phosphatidylcholine
Purity determination
title_short Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P
title_full Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P
title_fullStr Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P
title_full_unstemmed Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P
title_sort Caracterização da pureza de fosfatidilcolina da soja através de RMN de 1H e de 31P
author Mertins, Omar
author_facet Mertins, Omar
Sebben, Marcelo
Schneider, Paulo Henrique
Pohlmann, Adriana Raffin
Silveira, Nádya Pesce da
author_role author
author2 Sebben, Marcelo
Schneider, Paulo Henrique
Pohlmann, Adriana Raffin
Silveira, Nádya Pesce da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mertins, Omar
Sebben, Marcelo
Schneider, Paulo Henrique
Pohlmann, Adriana Raffin
Silveira, Nádya Pesce da
dc.subject.por.fl_str_mv Ressonância magnética nuclear
Lecitina de soja
topic Ressonância magnética nuclear
Lecitina de soja
NMR
Phosphatidylcholine
Purity determination
dc.subject.eng.fl_str_mv NMR
Phosphatidylcholine
Purity determination
description A strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of 1H NMR spectra. Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm. Before purification, a ratio of 19.68¡ ±1.37 was determined. Using extraction, a ratio of 10.70¡ ±0.61 was found, while from column chromatography, a value of 2.99¡ ±0.25 was detected. 31P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.
publishDate 2008
dc.date.issued.fl_str_mv 2008
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dc.relation.ispartof.pt_BR.fl_str_mv Química nova. São Paulo. Vol. 31, n. 7 (2008), p. 1856-1859
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