Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P

Detalhes bibliográficos
Autor(a) principal: Mertins,Omar
Data de Publicação: 2008
Outros Autores: Sebben,Marcelo, Henrique Schneider,Paulo, Pohlmann,Adriana Raffin, Silveira,Nádya Pesce da
Tipo de documento: Artigo
Idioma: por
Título da fonte: Química Nova (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000700043
Resumo: A strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of ¹H NMR spectra. Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm. Before purification, a ratio of 19.68±1.37 was determined. Using extraction, a ratio of 10.70±0.61 was found, while from column chromatography, a value of 2.99±0.25 was detected. 31P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.
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spelling Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31PNMRphosphatidylcholinepurity determinationA strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of ¹H NMR spectra. Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm. Before purification, a ratio of 19.68±1.37 was determined. Using extraction, a ratio of 10.70±0.61 was found, while from column chromatography, a value of 2.99±0.25 was detected. 31P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.Sociedade Brasileira de Química2008-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000700043Química Nova v.31 n.7 2008reponame:Química Nova (Online)instname:Sociedade Brasileira de Química (SBQ)instacron:SBQ10.1590/S0100-40422008000700043info:eu-repo/semantics/openAccessMertins,OmarSebben,MarceloHenrique Schneider,PauloPohlmann,Adriana RaffinSilveira,Nádya Pesce dapor2008-12-09T00:00:00Zoai:scielo:S0100-40422008000700043Revistahttps://www.scielo.br/j/qn/ONGhttps://old.scielo.br/oai/scielo-oai.phpquimicanova@sbq.org.br1678-70640100-4042opendoar:2008-12-09T00:00Química Nova (Online) - Sociedade Brasileira de Química (SBQ)false
dc.title.none.fl_str_mv Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
title Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
spellingShingle Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
Mertins,Omar
NMR
phosphatidylcholine
purity determination
title_short Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
title_full Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
title_fullStr Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
title_full_unstemmed Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
title_sort Caracterização da pureza de fosfatidilcolina da soja através de RMN de ¹H e de 31P
author Mertins,Omar
author_facet Mertins,Omar
Sebben,Marcelo
Henrique Schneider,Paulo
Pohlmann,Adriana Raffin
Silveira,Nádya Pesce da
author_role author
author2 Sebben,Marcelo
Henrique Schneider,Paulo
Pohlmann,Adriana Raffin
Silveira,Nádya Pesce da
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Mertins,Omar
Sebben,Marcelo
Henrique Schneider,Paulo
Pohlmann,Adriana Raffin
Silveira,Nádya Pesce da
dc.subject.por.fl_str_mv NMR
phosphatidylcholine
purity determination
topic NMR
phosphatidylcholine
purity determination
description A strategy is proposed to evaluate the purity of phosphatidylcholine from soybean lecithin, obtained by extraction or column chromatography, using the integrals ratio of ¹H NMR spectra. Integrals of methylene signals, around 1.3 and 1.6 ppm, are added and divided by the integral of the choline methyl groups, around 3.3 ppm. Before purification, a ratio of 19.68±1.37 was determined. Using extraction, a ratio of 10.70±0.61 was found, while from column chromatography, a value of 2.99±0.25 was detected. 31P NMR of standard phosphatidylcholine showed signals at -0.2 and -0.9 ppm, whereas the purified one showed a single signal at -0.9 ppm.
publishDate 2008
dc.date.none.fl_str_mv 2008-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000700043
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422008000700043
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv 10.1590/S0100-40422008000700043
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Química
publisher.none.fl_str_mv Sociedade Brasileira de Química
dc.source.none.fl_str_mv Química Nova v.31 n.7 2008
reponame:Química Nova (Online)
instname:Sociedade Brasileira de Química (SBQ)
instacron:SBQ
instname_str Sociedade Brasileira de Química (SBQ)
instacron_str SBQ
institution SBQ
reponame_str Química Nova (Online)
collection Química Nova (Online)
repository.name.fl_str_mv Química Nova (Online) - Sociedade Brasileira de Química (SBQ)
repository.mail.fl_str_mv quimicanova@sbq.org.br
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