Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/224774 |
Resumo: | Cardiac iron overload is directly associated with cardiac dysfunction and can ultimately lead to heart failure. This study examined the effect of secoisolariciresinol diglucoside (SDG), a component of flaxseed, on iron overload induced cardiac damage by evaluating oxidative stress, inflammation and apoptosis in H9c2 cardiomyocytes. Cells were incubated with 50 μ5M iron for 24 hours and/or a 24 hour pre-treatment of 500 μ M SDG. Cardiac iron overload resulted in increased oxidative stress and gene expression of the inflammatory mediators tumor necrosis factor-α, interleukin-10 and interferon γ, as well as matrix metalloproteinases-2 and -9. Increased apoptosis was evident by increased active caspase 3/7 activity and increased protein expression of Forkhead box O3a, caspase 3 and Bax. Cardiac iron overload also resulted in increased protein expression of p70S6 Kinase 1 and decreased expression of AMP-activated protein kinase. Pre-treatment with SDG abrogated the iron-induced increases in oxidative stress, inflammation and apoptosis, as well as the increased p70S6 Kinase 1 and decreased AMP-activated protein kinase expression. The decrease in superoxide dismutase activity by iron treatment was prevented by pre-treatment with SDG in the presence of iron. Based on these findings we conclude that SDG was cytoprotective in an in vitro model of iron overload induced redox-inflammatory damage, suggesting a novel potential role for SDG in cardiac iron overload. |
id |
UFRGS-2_8061a18cbf98a5e5c9cede60406b8d61 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/224774 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Puukila, StephanieBryan, SeanLaakso, AnnaAbdel-Malak, JessicaGurney, CarliAgostino, AdrianBelló-Klein, AdrianePrasad, KailashKhaper, Neelam2021-07-29T04:31:23Z20151932-6203http://hdl.handle.net/10183/224774001058162Cardiac iron overload is directly associated with cardiac dysfunction and can ultimately lead to heart failure. This study examined the effect of secoisolariciresinol diglucoside (SDG), a component of flaxseed, on iron overload induced cardiac damage by evaluating oxidative stress, inflammation and apoptosis in H9c2 cardiomyocytes. Cells were incubated with 50 μ5M iron for 24 hours and/or a 24 hour pre-treatment of 500 μ M SDG. Cardiac iron overload resulted in increased oxidative stress and gene expression of the inflammatory mediators tumor necrosis factor-α, interleukin-10 and interferon γ, as well as matrix metalloproteinases-2 and -9. Increased apoptosis was evident by increased active caspase 3/7 activity and increased protein expression of Forkhead box O3a, caspase 3 and Bax. Cardiac iron overload also resulted in increased protein expression of p70S6 Kinase 1 and decreased expression of AMP-activated protein kinase. Pre-treatment with SDG abrogated the iron-induced increases in oxidative stress, inflammation and apoptosis, as well as the increased p70S6 Kinase 1 and decreased AMP-activated protein kinase expression. The decrease in superoxide dismutase activity by iron treatment was prevented by pre-treatment with SDG in the presence of iron. Based on these findings we conclude that SDG was cytoprotective in an in vitro model of iron overload induced redox-inflammatory damage, suggesting a novel potential role for SDG in cardiac iron overload.application/pdfengPloS one. San Francisco. Vol. 10, no. 3 (Mar. 2015), e0122852, 16 f.Sobrecarga de ferroEstresse oxidativoCardiopatiasSecoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload conditionEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001058162.pdf.txt001058162.pdf.txtExtracted Texttext/plain42412http://www.lume.ufrgs.br/bitstream/10183/224774/2/001058162.pdf.txt0c5125b9885876464dc8f84094265318MD52ORIGINAL001058162.pdfTexto completo (inglês)application/pdf4851758http://www.lume.ufrgs.br/bitstream/10183/224774/1/001058162.pdfdf3ea07065b029ac35b94e10e2eb420fMD5110183/2247742023-01-20 06:01:36.865927oai:www.lume.ufrgs.br:10183/224774Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-01-20T08:01:36Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition |
title |
Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition |
spellingShingle |
Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition Puukila, Stephanie Sobrecarga de ferro Estresse oxidativo Cardiopatias |
title_short |
Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition |
title_full |
Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition |
title_fullStr |
Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition |
title_full_unstemmed |
Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition |
title_sort |
Secoisolariciresinol diglucoside abrogates oxidative stress-induced damage in cardiac iron overload condition |
author |
Puukila, Stephanie |
author_facet |
Puukila, Stephanie Bryan, Sean Laakso, Anna Abdel-Malak, Jessica Gurney, Carli Agostino, Adrian Belló-Klein, Adriane Prasad, Kailash Khaper, Neelam |
author_role |
author |
author2 |
Bryan, Sean Laakso, Anna Abdel-Malak, Jessica Gurney, Carli Agostino, Adrian Belló-Klein, Adriane Prasad, Kailash Khaper, Neelam |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Puukila, Stephanie Bryan, Sean Laakso, Anna Abdel-Malak, Jessica Gurney, Carli Agostino, Adrian Belló-Klein, Adriane Prasad, Kailash Khaper, Neelam |
dc.subject.por.fl_str_mv |
Sobrecarga de ferro Estresse oxidativo Cardiopatias |
topic |
Sobrecarga de ferro Estresse oxidativo Cardiopatias |
description |
Cardiac iron overload is directly associated with cardiac dysfunction and can ultimately lead to heart failure. This study examined the effect of secoisolariciresinol diglucoside (SDG), a component of flaxseed, on iron overload induced cardiac damage by evaluating oxidative stress, inflammation and apoptosis in H9c2 cardiomyocytes. Cells were incubated with 50 μ5M iron for 24 hours and/or a 24 hour pre-treatment of 500 μ M SDG. Cardiac iron overload resulted in increased oxidative stress and gene expression of the inflammatory mediators tumor necrosis factor-α, interleukin-10 and interferon γ, as well as matrix metalloproteinases-2 and -9. Increased apoptosis was evident by increased active caspase 3/7 activity and increased protein expression of Forkhead box O3a, caspase 3 and Bax. Cardiac iron overload also resulted in increased protein expression of p70S6 Kinase 1 and decreased expression of AMP-activated protein kinase. Pre-treatment with SDG abrogated the iron-induced increases in oxidative stress, inflammation and apoptosis, as well as the increased p70S6 Kinase 1 and decreased AMP-activated protein kinase expression. The decrease in superoxide dismutase activity by iron treatment was prevented by pre-treatment with SDG in the presence of iron. Based on these findings we conclude that SDG was cytoprotective in an in vitro model of iron overload induced redox-inflammatory damage, suggesting a novel potential role for SDG in cardiac iron overload. |
publishDate |
2015 |
dc.date.issued.fl_str_mv |
2015 |
dc.date.accessioned.fl_str_mv |
2021-07-29T04:31:23Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/224774 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001058162 |
identifier_str_mv |
1932-6203 001058162 |
url |
http://hdl.handle.net/10183/224774 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PloS one. San Francisco. Vol. 10, no. 3 (Mar. 2015), e0122852, 16 f. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/224774/2/001058162.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/224774/1/001058162.pdf |
bitstream.checksum.fl_str_mv |
0c5125b9885876464dc8f84094265318 df3ea07065b029ac35b94e10e2eb420f |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225029186224128 |