Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
Autor(a) principal: | |
---|---|
Data de Publicação: | 2002 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/218319 |
Resumo: | The mechanisms by which phenylalanine is toxic to the brain in phenylketonuria are not fully understood. Considering that brain glucose metabolism is reduced in these patients, our main objective was to determine pyruvate kinase activity in brain cortex of rats subjected to acute and chronic chemically induced hyperphenylalaninemia. The effect of alanine administration on the enzyme activity in the treated rats was also investigated. We also studied the in vitro effect of the two amino acids on pyruvate kinase activity in brain cortex of nontreated rats. The results indicated that phenylalanine inhibits pyruvate kinase in vitro and in vivo and that alanine prevents the inhibitory effect of phenylalanine on the enzyme activity. Considering the crucial role pyruvate kinase plays in glucose metabolism in brain, it is possible that inhibition of this enzyme activity may contribute to the brain damage characteristic of this disease. |
id |
UFRGS-2_81e16455e6ccdba13fdb39254a188a18 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/218319 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Feksa, Luciane RosaCornelio, Andrea RenataRech, Virgínia CieloWannmacher, Clovis Milton DuvalDutra Filho, Carlos SeveroWyse, Angela Terezinha de SouzaWajner, Moacir2021-03-02T04:16:07Z20020364-3190http://hdl.handle.net/10183/218319000386183The mechanisms by which phenylalanine is toxic to the brain in phenylketonuria are not fully understood. Considering that brain glucose metabolism is reduced in these patients, our main objective was to determine pyruvate kinase activity in brain cortex of rats subjected to acute and chronic chemically induced hyperphenylalaninemia. The effect of alanine administration on the enzyme activity in the treated rats was also investigated. We also studied the in vitro effect of the two amino acids on pyruvate kinase activity in brain cortex of nontreated rats. The results indicated that phenylalanine inhibits pyruvate kinase in vitro and in vivo and that alanine prevents the inhibitory effect of phenylalanine on the enzyme activity. Considering the crucial role pyruvate kinase plays in glucose metabolism in brain, it is possible that inhibition of this enzyme activity may contribute to the brain damage characteristic of this disease.application/pdfengNeurochemical research. New York, NY. Vol. 27, no. 9 (2002), p. 947-952FenilalaninaFenilcetonúriasAlaninaPiruvato QuinasePhenylalaninePhenylketonuriaHyperphenylalaninemiaAlaninePyruvate kinaseAlanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemiaEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000386183.pdf.txt000386183.pdf.txtExtracted Texttext/plain0http://www.lume.ufrgs.br/bitstream/10183/218319/2/000386183.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD52ORIGINAL000386183.pdfTexto completo (inglês)application/pdf1635420http://www.lume.ufrgs.br/bitstream/10183/218319/1/000386183.pdf3a63a3566c14293ecc3dd21ab94145deMD5110183/2183192021-04-12 08:46:02.990423oai:www.lume.ufrgs.br:10183/218319Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-04-12T11:46:02Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia |
title |
Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia |
spellingShingle |
Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia Feksa, Luciane Rosa Fenilalanina Fenilcetonúrias Alanina Piruvato Quinase Phenylalanine Phenylketonuria Hyperphenylalaninemia Alanine Pyruvate kinase |
title_short |
Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia |
title_full |
Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia |
title_fullStr |
Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia |
title_full_unstemmed |
Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia |
title_sort |
Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia |
author |
Feksa, Luciane Rosa |
author_facet |
Feksa, Luciane Rosa Cornelio, Andrea Renata Rech, Virgínia Cielo Wannmacher, Clovis Milton Duval Dutra Filho, Carlos Severo Wyse, Angela Terezinha de Souza Wajner, Moacir |
author_role |
author |
author2 |
Cornelio, Andrea Renata Rech, Virgínia Cielo Wannmacher, Clovis Milton Duval Dutra Filho, Carlos Severo Wyse, Angela Terezinha de Souza Wajner, Moacir |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Feksa, Luciane Rosa Cornelio, Andrea Renata Rech, Virgínia Cielo Wannmacher, Clovis Milton Duval Dutra Filho, Carlos Severo Wyse, Angela Terezinha de Souza Wajner, Moacir |
dc.subject.por.fl_str_mv |
Fenilalanina Fenilcetonúrias Alanina Piruvato Quinase |
topic |
Fenilalanina Fenilcetonúrias Alanina Piruvato Quinase Phenylalanine Phenylketonuria Hyperphenylalaninemia Alanine Pyruvate kinase |
dc.subject.eng.fl_str_mv |
Phenylalanine Phenylketonuria Hyperphenylalaninemia Alanine Pyruvate kinase |
description |
The mechanisms by which phenylalanine is toxic to the brain in phenylketonuria are not fully understood. Considering that brain glucose metabolism is reduced in these patients, our main objective was to determine pyruvate kinase activity in brain cortex of rats subjected to acute and chronic chemically induced hyperphenylalaninemia. The effect of alanine administration on the enzyme activity in the treated rats was also investigated. We also studied the in vitro effect of the two amino acids on pyruvate kinase activity in brain cortex of nontreated rats. The results indicated that phenylalanine inhibits pyruvate kinase in vitro and in vivo and that alanine prevents the inhibitory effect of phenylalanine on the enzyme activity. Considering the crucial role pyruvate kinase plays in glucose metabolism in brain, it is possible that inhibition of this enzyme activity may contribute to the brain damage characteristic of this disease. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2021-03-02T04:16:07Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/218319 |
dc.identifier.issn.pt_BR.fl_str_mv |
0364-3190 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000386183 |
identifier_str_mv |
0364-3190 000386183 |
url |
http://hdl.handle.net/10183/218319 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Neurochemical research. New York, NY. Vol. 27, no. 9 (2002), p. 947-952 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/218319/2/000386183.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/218319/1/000386183.pdf |
bitstream.checksum.fl_str_mv |
d41d8cd98f00b204e9800998ecf8427e 3a63a3566c14293ecc3dd21ab94145de |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1815447732679081984 |