Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia

Detalhes bibliográficos
Autor(a) principal: Feksa, Luciane Rosa
Data de Publicação: 2002
Outros Autores: Cornelio, Andrea Renata, Rech, Virgínia Cielo, Wannmacher, Clovis Milton Duval, Dutra Filho, Carlos Severo, Wyse, Angela Terezinha de Souza, Wajner, Moacir
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/218319
Resumo: The mechanisms by which phenylalanine is toxic to the brain in phenylketonuria are not fully understood. Considering that brain glucose metabolism is reduced in these patients, our main objective was to determine pyruvate kinase activity in brain cortex of rats subjected to acute and chronic chemically induced hyperphenylalaninemia. The effect of alanine administration on the enzyme activity in the treated rats was also investigated. We also studied the in vitro effect of the two amino acids on pyruvate kinase activity in brain cortex of nontreated rats. The results indicated that phenylalanine inhibits pyruvate kinase in vitro and in vivo and that alanine prevents the inhibitory effect of phenylalanine on the enzyme activity. Considering the crucial role pyruvate kinase plays in glucose metabolism in brain, it is possible that inhibition of this enzyme activity may contribute to the brain damage characteristic of this disease.
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spelling Feksa, Luciane RosaCornelio, Andrea RenataRech, Virgínia CieloWannmacher, Clovis Milton DuvalDutra Filho, Carlos SeveroWyse, Angela Terezinha de SouzaWajner, Moacir2021-03-02T04:16:07Z20020364-3190http://hdl.handle.net/10183/218319000386183The mechanisms by which phenylalanine is toxic to the brain in phenylketonuria are not fully understood. Considering that brain glucose metabolism is reduced in these patients, our main objective was to determine pyruvate kinase activity in brain cortex of rats subjected to acute and chronic chemically induced hyperphenylalaninemia. The effect of alanine administration on the enzyme activity in the treated rats was also investigated. We also studied the in vitro effect of the two amino acids on pyruvate kinase activity in brain cortex of nontreated rats. The results indicated that phenylalanine inhibits pyruvate kinase in vitro and in vivo and that alanine prevents the inhibitory effect of phenylalanine on the enzyme activity. Considering the crucial role pyruvate kinase plays in glucose metabolism in brain, it is possible that inhibition of this enzyme activity may contribute to the brain damage characteristic of this disease.application/pdfengNeurochemical research. New York, NY. Vol. 27, no. 9 (2002), p. 947-952FenilalaninaFenilcetonúriasAlaninaPiruvato QuinasePhenylalaninePhenylketonuriaHyperphenylalaninemiaAlaninePyruvate kinaseAlanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemiaEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000386183.pdf.txt000386183.pdf.txtExtracted Texttext/plain0http://www.lume.ufrgs.br/bitstream/10183/218319/2/000386183.pdf.txtd41d8cd98f00b204e9800998ecf8427eMD52ORIGINAL000386183.pdfTexto completo (inglês)application/pdf1635420http://www.lume.ufrgs.br/bitstream/10183/218319/1/000386183.pdf3a63a3566c14293ecc3dd21ab94145deMD5110183/2183192021-04-12 08:46:02.990423oai:www.lume.ufrgs.br:10183/218319Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-04-12T11:46:02Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
title Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
spellingShingle Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
Feksa, Luciane Rosa
Fenilalanina
Fenilcetonúrias
Alanina
Piruvato Quinase
Phenylalanine
Phenylketonuria
Hyperphenylalaninemia
Alanine
Pyruvate kinase
title_short Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
title_full Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
title_fullStr Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
title_full_unstemmed Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
title_sort Alanine prevents the reduction of pyruvate kinase activity in brain cortex of rats subjected to chemically induced hyperphenylalaninemia
author Feksa, Luciane Rosa
author_facet Feksa, Luciane Rosa
Cornelio, Andrea Renata
Rech, Virgínia Cielo
Wannmacher, Clovis Milton Duval
Dutra Filho, Carlos Severo
Wyse, Angela Terezinha de Souza
Wajner, Moacir
author_role author
author2 Cornelio, Andrea Renata
Rech, Virgínia Cielo
Wannmacher, Clovis Milton Duval
Dutra Filho, Carlos Severo
Wyse, Angela Terezinha de Souza
Wajner, Moacir
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Feksa, Luciane Rosa
Cornelio, Andrea Renata
Rech, Virgínia Cielo
Wannmacher, Clovis Milton Duval
Dutra Filho, Carlos Severo
Wyse, Angela Terezinha de Souza
Wajner, Moacir
dc.subject.por.fl_str_mv Fenilalanina
Fenilcetonúrias
Alanina
Piruvato Quinase
topic Fenilalanina
Fenilcetonúrias
Alanina
Piruvato Quinase
Phenylalanine
Phenylketonuria
Hyperphenylalaninemia
Alanine
Pyruvate kinase
dc.subject.eng.fl_str_mv Phenylalanine
Phenylketonuria
Hyperphenylalaninemia
Alanine
Pyruvate kinase
description The mechanisms by which phenylalanine is toxic to the brain in phenylketonuria are not fully understood. Considering that brain glucose metabolism is reduced in these patients, our main objective was to determine pyruvate kinase activity in brain cortex of rats subjected to acute and chronic chemically induced hyperphenylalaninemia. The effect of alanine administration on the enzyme activity in the treated rats was also investigated. We also studied the in vitro effect of the two amino acids on pyruvate kinase activity in brain cortex of nontreated rats. The results indicated that phenylalanine inhibits pyruvate kinase in vitro and in vivo and that alanine prevents the inhibitory effect of phenylalanine on the enzyme activity. Considering the crucial role pyruvate kinase plays in glucose metabolism in brain, it is possible that inhibition of this enzyme activity may contribute to the brain damage characteristic of this disease.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2021-03-02T04:16:07Z
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dc.identifier.issn.pt_BR.fl_str_mv 0364-3190
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dc.relation.ispartof.pt_BR.fl_str_mv Neurochemical research. New York, NY. Vol. 27, no. 9 (2002), p. 947-952
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