Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/115719 |
Resumo: | Objectives: To verify whether changes in bone mass induced by glucocorticoid and bisphosphonate can be detected by digital subtraction radiography of lateral X-rays in female rat mandibles. Methods: 36 Rattus norvegicus (Wistar) females were randomly allocated to three groups: Group 1, control (n512); Group 2, induced osteopaenia (n512); and Group 3, induced osteopaenia plus risedronate (n512). Group 1 received subcutaneous saline solution (2 ml kg21) injections for 12 weeks. Group 2 received 1 mg kg21 methylprednisolone acetate for 12 weeks. Group 3 received the same treatment as Group 2, plus 3 mg kg21 risedronate sodium for a further 12 weeks. A radiograph of the left mandible was taken on day 0 and after 12 weeks. A third radiograph was taken in Group 3 after risedronate treatment. Digital subtraction of the radiographs was used to compare the intensity of pixels in the control area and the test area. The animals were killed and the same area assessed by subtraction was histologically evaluated. Results: ANOVA revealed statistically significant histological differences between the three groups. Tukey’s multiple comparisons test showed that Group 2 had the smallest mean proportion of bone trabeculae per field and Group 3 the largest (F537.56; P , 0.01). Radiographic subtraction revealed a higher mean pixel intensity in Groups 1 and 3 vs Group 2. In Group 3, glucocorticoid caused a significant loss in radiographic density, and risedronate restored that loss (Friedman’s non-parametric test). Conclusions: Subtraction radiography was able to detect changes in bone mass induced by glucocorticoid and bisphosphonate. |
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Mahl, Célia Regina WinckFontanella, Vania Regina Camargo2015-05-01T01:58:07Z20080250-832Xhttp://hdl.handle.net/10183/115719000952392Objectives: To verify whether changes in bone mass induced by glucocorticoid and bisphosphonate can be detected by digital subtraction radiography of lateral X-rays in female rat mandibles. Methods: 36 Rattus norvegicus (Wistar) females were randomly allocated to three groups: Group 1, control (n512); Group 2, induced osteopaenia (n512); and Group 3, induced osteopaenia plus risedronate (n512). Group 1 received subcutaneous saline solution (2 ml kg21) injections for 12 weeks. Group 2 received 1 mg kg21 methylprednisolone acetate for 12 weeks. Group 3 received the same treatment as Group 2, plus 3 mg kg21 risedronate sodium for a further 12 weeks. A radiograph of the left mandible was taken on day 0 and after 12 weeks. A third radiograph was taken in Group 3 after risedronate treatment. Digital subtraction of the radiographs was used to compare the intensity of pixels in the control area and the test area. The animals were killed and the same area assessed by subtraction was histologically evaluated. Results: ANOVA revealed statistically significant histological differences between the three groups. Tukey’s multiple comparisons test showed that Group 2 had the smallest mean proportion of bone trabeculae per field and Group 3 the largest (F537.56; P , 0.01). Radiographic subtraction revealed a higher mean pixel intensity in Groups 1 and 3 vs Group 2. In Group 3, glucocorticoid caused a significant loss in radiographic density, and risedronate restored that loss (Friedman’s non-parametric test). Conclusions: Subtraction radiography was able to detect changes in bone mass induced by glucocorticoid and bisphosphonate.application/pdfengDentomaxillofacial radiology. Oxford, UK. Vol. 37, no. 8 (Dec. 2008), p. 438-444RadiografiaMandíbulaRatos WistarOsteoporoseOdontologiaBisphosphonatesGlucocorticoidsSubtraction techniqueOsteoporosisEvaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandibleEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000952392.pdf000952392.pdfTexto completo (inglês)application/pdf235031http://www.lume.ufrgs.br/bitstream/10183/115719/1/000952392.pdfdac3044bb6f7006bf29e0dfec476b44fMD51TEXT000952392.pdf.txt000952392.pdf.txtExtracted Texttext/plain32095http://www.lume.ufrgs.br/bitstream/10183/115719/2/000952392.pdf.txt56ed02e3a6301bab81abf93aaca069b3MD52THUMBNAIL000952392.pdf.jpg000952392.pdf.jpgGenerated Thumbnailimage/jpeg1778http://www.lume.ufrgs.br/bitstream/10183/115719/3/000952392.pdf.jpg4018ea9f360a3f6bc3372bed652cbecbMD5310183/1157192018-10-22 07:38:49.251oai:www.lume.ufrgs.br:10183/115719Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-22T10:38:49Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible |
title |
Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible |
spellingShingle |
Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible Mahl, Célia Regina Winck Radiografia Mandíbula Ratos Wistar Osteoporose Odontologia Bisphosphonates Glucocorticoids Subtraction technique Osteoporosis |
title_short |
Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible |
title_full |
Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible |
title_fullStr |
Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible |
title_full_unstemmed |
Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible |
title_sort |
Evaluation by digital subtraction radiography of induced changes in the bone density of the female rat mandible |
author |
Mahl, Célia Regina Winck |
author_facet |
Mahl, Célia Regina Winck Fontanella, Vania Regina Camargo |
author_role |
author |
author2 |
Fontanella, Vania Regina Camargo |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Mahl, Célia Regina Winck Fontanella, Vania Regina Camargo |
dc.subject.por.fl_str_mv |
Radiografia Mandíbula Ratos Wistar Osteoporose Odontologia |
topic |
Radiografia Mandíbula Ratos Wistar Osteoporose Odontologia Bisphosphonates Glucocorticoids Subtraction technique Osteoporosis |
dc.subject.eng.fl_str_mv |
Bisphosphonates Glucocorticoids Subtraction technique Osteoporosis |
description |
Objectives: To verify whether changes in bone mass induced by glucocorticoid and bisphosphonate can be detected by digital subtraction radiography of lateral X-rays in female rat mandibles. Methods: 36 Rattus norvegicus (Wistar) females were randomly allocated to three groups: Group 1, control (n512); Group 2, induced osteopaenia (n512); and Group 3, induced osteopaenia plus risedronate (n512). Group 1 received subcutaneous saline solution (2 ml kg21) injections for 12 weeks. Group 2 received 1 mg kg21 methylprednisolone acetate for 12 weeks. Group 3 received the same treatment as Group 2, plus 3 mg kg21 risedronate sodium for a further 12 weeks. A radiograph of the left mandible was taken on day 0 and after 12 weeks. A third radiograph was taken in Group 3 after risedronate treatment. Digital subtraction of the radiographs was used to compare the intensity of pixels in the control area and the test area. The animals were killed and the same area assessed by subtraction was histologically evaluated. Results: ANOVA revealed statistically significant histological differences between the three groups. Tukey’s multiple comparisons test showed that Group 2 had the smallest mean proportion of bone trabeculae per field and Group 3 the largest (F537.56; P , 0.01). Radiographic subtraction revealed a higher mean pixel intensity in Groups 1 and 3 vs Group 2. In Group 3, glucocorticoid caused a significant loss in radiographic density, and risedronate restored that loss (Friedman’s non-parametric test). Conclusions: Subtraction radiography was able to detect changes in bone mass induced by glucocorticoid and bisphosphonate. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
dc.date.accessioned.fl_str_mv |
2015-05-01T01:58:07Z |
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000952392 |
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dc.language.iso.fl_str_mv |
eng |
language |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Dentomaxillofacial radiology. Oxford, UK. Vol. 37, no. 8 (Dec. 2008), p. 438-444 |
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