Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain

Detalhes bibliográficos
Autor(a) principal: Siqueira, Franciele Maboni
Data de Publicação: 2018
Outros Autores: Cibulski, Samuel Paulo, Mayer, Fabiana Quoos, Driemeier, David, Pavarini, Saulo Petinatti, Vargas, Agueda Castagna de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/174326
Resumo: Bacillus anthracis strain SPV842 15 was isolated from bovine fetus, while B. anthracis strainBrazilian vaccinal was recovered from a commercial vaccine. We report here the genomesequences of both strains. The SPV842 15 genome is composed of a single circular chromo-some with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn,the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Bothgenomes have an overall C + G content of 35.4%, and 11 genes encoding the ribosomal RNAs(rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxinssynthesis, was detected and completely assembled for both strains. These plasmids havea length of 181,684 base pairs and a C + G content of 32.5%. These genomic data generateinsights about vaccinal B. anthracis virulence.
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spelling Siqueira, Franciele MaboniCibulski, Samuel PauloMayer, Fabiana QuoosDriemeier, DavidPavarini, Saulo PetinattiVargas, Agueda Castagna de2018-04-03T02:26:17Z20181678-4405http://hdl.handle.net/10183/174326001062876Bacillus anthracis strain SPV842 15 was isolated from bovine fetus, while B. anthracis strainBrazilian vaccinal was recovered from a commercial vaccine. We report here the genomesequences of both strains. The SPV842 15 genome is composed of a single circular chromo-some with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn,the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Bothgenomes have an overall C + G content of 35.4%, and 11 genes encoding the ribosomal RNAs(rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxinssynthesis, was detected and completely assembled for both strains. These plasmids havea length of 181,684 base pairs and a C + G content of 32.5%. These genomic data generateinsights about vaccinal B. anthracis virulence.application/pdfengBrazilian Journal of Microbiology. São Paulo. Vol. 49, no. 1 (Jan. 2018), p. 18-19Análise de sequência de DNASequenciamento de nova geraçãoBacillus anthracisAntrazPatogenicidadeVacinasFeto abortadoBovinosAnthraxVaccine pathogenicityBovine abortionNext generation sequencingaGenome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal straininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001062876.pdf001062876.pdfTexto completo (inglês)application/pdf344649http://www.lume.ufrgs.br/bitstream/10183/174326/1/001062876.pdf60418627da539b37771eca05f205291fMD51TEXT001062876.pdf.txt001062876.pdf.txtExtracted Texttext/plain6975http://www.lume.ufrgs.br/bitstream/10183/174326/2/001062876.pdf.txt941e43f4971e64dc0584ff6dd7535198MD52THUMBNAIL001062876.pdf.jpg001062876.pdf.jpgGenerated Thumbnailimage/jpeg2090http://www.lume.ufrgs.br/bitstream/10183/174326/3/001062876.pdf.jpg5c29bb820bc1347e100312f6c0271c16MD5310183/1743262019-05-12 02:36:26.067011oai:www.lume.ufrgs.br:10183/174326Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-05-12T05:36:26Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain
title Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain
spellingShingle Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain
Siqueira, Franciele Maboni
Análise de sequência de DNA
Sequenciamento de nova geração
Bacillus anthracis
Antraz
Patogenicidade
Vacinas
Feto abortado
Bovinos
Anthrax
Vaccine pathogenicity
Bovine abortion
Next generation sequencinga
title_short Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain
title_full Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain
title_fullStr Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain
title_full_unstemmed Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain
title_sort Genome sequencing of two Bacillus anthracis strains : a virulent strain and a vaccinal strain
author Siqueira, Franciele Maboni
author_facet Siqueira, Franciele Maboni
Cibulski, Samuel Paulo
Mayer, Fabiana Quoos
Driemeier, David
Pavarini, Saulo Petinatti
Vargas, Agueda Castagna de
author_role author
author2 Cibulski, Samuel Paulo
Mayer, Fabiana Quoos
Driemeier, David
Pavarini, Saulo Petinatti
Vargas, Agueda Castagna de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Siqueira, Franciele Maboni
Cibulski, Samuel Paulo
Mayer, Fabiana Quoos
Driemeier, David
Pavarini, Saulo Petinatti
Vargas, Agueda Castagna de
dc.subject.por.fl_str_mv Análise de sequência de DNA
Sequenciamento de nova geração
Bacillus anthracis
Antraz
Patogenicidade
Vacinas
Feto abortado
Bovinos
topic Análise de sequência de DNA
Sequenciamento de nova geração
Bacillus anthracis
Antraz
Patogenicidade
Vacinas
Feto abortado
Bovinos
Anthrax
Vaccine pathogenicity
Bovine abortion
Next generation sequencinga
dc.subject.eng.fl_str_mv Anthrax
Vaccine pathogenicity
Bovine abortion
Next generation sequencinga
description Bacillus anthracis strain SPV842 15 was isolated from bovine fetus, while B. anthracis strainBrazilian vaccinal was recovered from a commercial vaccine. We report here the genomesequences of both strains. The SPV842 15 genome is composed of a single circular chromo-some with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn,the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Bothgenomes have an overall C + G content of 35.4%, and 11 genes encoding the ribosomal RNAs(rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxinssynthesis, was detected and completely assembled for both strains. These plasmids havea length of 181,684 base pairs and a C + G content of 32.5%. These genomic data generateinsights about vaccinal B. anthracis virulence.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-04-03T02:26:17Z
dc.date.issued.fl_str_mv 2018
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/174326
dc.identifier.issn.pt_BR.fl_str_mv 1678-4405
dc.identifier.nrb.pt_BR.fl_str_mv 001062876
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url http://hdl.handle.net/10183/174326
dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian Journal of Microbiology. São Paulo. Vol. 49, no. 1 (Jan. 2018), p. 18-19
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