Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain

Detalhes bibliográficos
Autor(a) principal: Siqueira,Franciele Maboni
Data de Publicação: 2018
Outros Autores: Cibulski,Samuel Paulo, Mayer,Fabiana Quoos, Driemeier,David, Pavarini,Saulo Petinatti, Vargas,Agueda Palmira Castagna de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100018
Resumo: ABSTRACT Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn, the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Both genomes have an overall C + G content of 35.4%, and 11 genes encoding the ribosomal RNAs (rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxins synthesis, was detected and completely assembled for both strains. These plasmids have a length of 181,684 base pairs and a C + G content of 32.5%. These genomic data generate insights about vaccinal B. anthracis virulence.
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spelling Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strainAnthraxVaccine pathogenicityBovine abortionNext generation sequencingABSTRACT Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn, the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Both genomes have an overall C + G content of 35.4%, and 11 genes encoding the ribosomal RNAs (rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxins synthesis, was detected and completely assembled for both strains. These plasmids have a length of 181,684 base pairs and a C + G content of 32.5%. These genomic data generate insights about vaccinal B. anthracis virulence.Sociedade Brasileira de Microbiologia2018-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100018Brazilian Journal of Microbiology v.49 n.1 2018reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2017.04.007info:eu-repo/semantics/openAccessSiqueira,Franciele MaboniCibulski,Samuel PauloMayer,Fabiana QuoosDriemeier,DavidPavarini,Saulo PetinattiVargas,Agueda Palmira Castagna deeng2018-02-20T00:00:00Zoai:scielo:S1517-83822018000100018Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2018-02-20T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
title Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
spellingShingle Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
Siqueira,Franciele Maboni
Anthrax
Vaccine pathogenicity
Bovine abortion
Next generation sequencing
title_short Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
title_full Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
title_fullStr Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
title_full_unstemmed Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
title_sort Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain
author Siqueira,Franciele Maboni
author_facet Siqueira,Franciele Maboni
Cibulski,Samuel Paulo
Mayer,Fabiana Quoos
Driemeier,David
Pavarini,Saulo Petinatti
Vargas,Agueda Palmira Castagna de
author_role author
author2 Cibulski,Samuel Paulo
Mayer,Fabiana Quoos
Driemeier,David
Pavarini,Saulo Petinatti
Vargas,Agueda Palmira Castagna de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Siqueira,Franciele Maboni
Cibulski,Samuel Paulo
Mayer,Fabiana Quoos
Driemeier,David
Pavarini,Saulo Petinatti
Vargas,Agueda Palmira Castagna de
dc.subject.por.fl_str_mv Anthrax
Vaccine pathogenicity
Bovine abortion
Next generation sequencing
topic Anthrax
Vaccine pathogenicity
Bovine abortion
Next generation sequencing
description ABSTRACT Bacillus anthracis strain SPV842_15 was isolated from bovine fetus, while B. anthracis strain Brazilian vaccinal was recovered from a commercial vaccine. We report here the genome sequences of both strains. The SPV842_15 genome is composed of a single circular chromosome with a length of 5,228,664 base pairs, and comprises 5911 coding sequences. In turn, the Brazilian vaccinal genome remains in 201 contigs with 5733 coding sequences. Both genomes have an overall C + G content of 35.4%, and 11 genes encoding the ribosomal RNAs (rRNAs) 5S, 16S and 23S. Only the plasmid pX01 sequence, which carries genes for toxins synthesis, was detected and completely assembled for both strains. These plasmids have a length of 181,684 base pairs and a C + G content of 32.5%. These genomic data generate insights about vaccinal B. anthracis virulence.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100018
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100018
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2017.04.007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.49 n.1 2018
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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