MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/267555 |
Resumo: | Microorganisms represent the most abundant biomass on the planet; however, because of several cultivation technique limitations, most of this genetic patrimony has been inaccessible. Due to the advent of metagenomic methodologies, such limitations have been overcome. Prevailing over these limitations enabled the genetic pool of non-cultivable microorganisms to be exploited for improvements in the development of biotechnological products. By utilising a metagenomic approach, we identifed a new gene related to biosurfactant production and hydrocarbon degradation. Environmental DNA was extracted from soil samples collected on the banks of the Jundiaí River (Natal, Brazil), and a metagenomic library was constructed. Functional screening identifed the clone 3C6, which was positive for the biosurfactant protein and revealed an open reading frame (ORF) with high similarity to sequences encoding a hypothetical protein from species of the family Halobacteriaceae. This protein was purifed and exhibited biosurfactant activity. Due to these properties, this protein was named metagenomic biosurfactant protein 1 (MBSP1). In addition, E. coli RosettaTM (DE3) strain cells transformed with the MBSP1 clone showed an increase in aliphatic hydrocarbon degradation. In this study, we described a single gene encoding a protein with marked tensoactive properties that can be produced in a host cell, such as Escherichia coli, without substrate dependence. Furthermore, MBSP1 has been demonstrated as the frst protein with these characteristics described in the Archaea or Bacteria domains. |
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Araújo, Sinara Carla da SilvaPortela, Rita de Cássia Barreto da SilvaLima, Daniel Chaves deFonseca, Marbella Maria BernardesAraujo, Wydemberg Jose deSilva, Uaska Bezerra eNapp, Amanda PasinatoPereira, José Evandro SaraivaVainstein, Marilene HenningLima, Lucymara Fassarella Agnez2023-11-24T03:24:23Z20202045-2322http://hdl.handle.net/10183/267555001175610Microorganisms represent the most abundant biomass on the planet; however, because of several cultivation technique limitations, most of this genetic patrimony has been inaccessible. Due to the advent of metagenomic methodologies, such limitations have been overcome. Prevailing over these limitations enabled the genetic pool of non-cultivable microorganisms to be exploited for improvements in the development of biotechnological products. By utilising a metagenomic approach, we identifed a new gene related to biosurfactant production and hydrocarbon degradation. Environmental DNA was extracted from soil samples collected on the banks of the Jundiaí River (Natal, Brazil), and a metagenomic library was constructed. Functional screening identifed the clone 3C6, which was positive for the biosurfactant protein and revealed an open reading frame (ORF) with high similarity to sequences encoding a hypothetical protein from species of the family Halobacteriaceae. This protein was purifed and exhibited biosurfactant activity. Due to these properties, this protein was named metagenomic biosurfactant protein 1 (MBSP1). In addition, E. coli RosettaTM (DE3) strain cells transformed with the MBSP1 clone showed an increase in aliphatic hydrocarbon degradation. In this study, we described a single gene encoding a protein with marked tensoactive properties that can be produced in a host cell, such as Escherichia coli, without substrate dependence. Furthermore, MBSP1 has been demonstrated as the frst protein with these characteristics described in the Archaea or Bacteria domains.application/pdfengScientific reports. London. Vol. 10 (Jan. 2020), 1340, 13 p.Escherichia coliGenética de microorganismosMBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradationEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001175610.pdf.txt001175610.pdf.txtExtracted Texttext/plain59890http://www.lume.ufrgs.br/bitstream/10183/267555/2/001175610.pdf.txt836f4074ac573bba4362df8f016292c6MD52ORIGINAL001175610.pdfTexto completo (inglês)application/pdf2781345http://www.lume.ufrgs.br/bitstream/10183/267555/1/001175610.pdfe434170d442f2a9b198870e6b2548594MD5110183/2675552023-11-25 04:26:30.898782oai:www.lume.ufrgs.br:10183/267555Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-11-25T06:26:30Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation |
title |
MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation |
spellingShingle |
MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation Araújo, Sinara Carla da Silva Escherichia coli Genética de microorganismos |
title_short |
MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation |
title_full |
MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation |
title_fullStr |
MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation |
title_full_unstemmed |
MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation |
title_sort |
MBSP1: a biosurfactant protein derived from a metagenomic library with activity in oil degradation |
author |
Araújo, Sinara Carla da Silva |
author_facet |
Araújo, Sinara Carla da Silva Portela, Rita de Cássia Barreto da Silva Lima, Daniel Chaves de Fonseca, Marbella Maria Bernardes Araujo, Wydemberg Jose de Silva, Uaska Bezerra e Napp, Amanda Pasinato Pereira, José Evandro Saraiva Vainstein, Marilene Henning Lima, Lucymara Fassarella Agnez |
author_role |
author |
author2 |
Portela, Rita de Cássia Barreto da Silva Lima, Daniel Chaves de Fonseca, Marbella Maria Bernardes Araujo, Wydemberg Jose de Silva, Uaska Bezerra e Napp, Amanda Pasinato Pereira, José Evandro Saraiva Vainstein, Marilene Henning Lima, Lucymara Fassarella Agnez |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Sinara Carla da Silva Portela, Rita de Cássia Barreto da Silva Lima, Daniel Chaves de Fonseca, Marbella Maria Bernardes Araujo, Wydemberg Jose de Silva, Uaska Bezerra e Napp, Amanda Pasinato Pereira, José Evandro Saraiva Vainstein, Marilene Henning Lima, Lucymara Fassarella Agnez |
dc.subject.por.fl_str_mv |
Escherichia coli Genética de microorganismos |
topic |
Escherichia coli Genética de microorganismos |
description |
Microorganisms represent the most abundant biomass on the planet; however, because of several cultivation technique limitations, most of this genetic patrimony has been inaccessible. Due to the advent of metagenomic methodologies, such limitations have been overcome. Prevailing over these limitations enabled the genetic pool of non-cultivable microorganisms to be exploited for improvements in the development of biotechnological products. By utilising a metagenomic approach, we identifed a new gene related to biosurfactant production and hydrocarbon degradation. Environmental DNA was extracted from soil samples collected on the banks of the Jundiaí River (Natal, Brazil), and a metagenomic library was constructed. Functional screening identifed the clone 3C6, which was positive for the biosurfactant protein and revealed an open reading frame (ORF) with high similarity to sequences encoding a hypothetical protein from species of the family Halobacteriaceae. This protein was purifed and exhibited biosurfactant activity. Due to these properties, this protein was named metagenomic biosurfactant protein 1 (MBSP1). In addition, E. coli RosettaTM (DE3) strain cells transformed with the MBSP1 clone showed an increase in aliphatic hydrocarbon degradation. In this study, we described a single gene encoding a protein with marked tensoactive properties that can be produced in a host cell, such as Escherichia coli, without substrate dependence. Furthermore, MBSP1 has been demonstrated as the frst protein with these characteristics described in the Archaea or Bacteria domains. |
publishDate |
2020 |
dc.date.issued.fl_str_mv |
2020 |
dc.date.accessioned.fl_str_mv |
2023-11-24T03:24:23Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10183/267555 |
dc.identifier.issn.pt_BR.fl_str_mv |
2045-2322 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001175610 |
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2045-2322 001175610 |
url |
http://hdl.handle.net/10183/267555 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Scientific reports. London. Vol. 10 (Jan. 2020), 1340, 13 p. |
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info:eu-repo/semantics/openAccess |
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openAccess |
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