Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants

Detalhes bibliográficos
Autor(a) principal: Granada, Camille Eichelberger
Data de Publicação: 2014
Outros Autores: Strochein, Marcos, Vargas, Luciano Kayser, Bruxel, Manuela, Sa, Enilson Luiz Saccol de, Passaglia, Luciane Maria Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/185928
Resumo: This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.
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spelling Granada, Camille EichelbergerStrochein, MarcosVargas, Luciano KayserBruxel, ManuelaSa, Enilson Luiz Saccol dePassaglia, Luciane Maria Pereira2018-12-05T02:44:14Z20141415-4757http://hdl.handle.net/10183/185928000933472This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.application/pdfengGenetics and molecular biology. Ribeirão Preto, SP. Vol. 37, no. 2, (abr./jun. 2014), p. 396-405RhizobiumNodulacaoNitrogênioDesmodium incanumRhizobiaNodulationNitrogenSpecificityGenetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plantsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000933472.pdf.txt000933472.pdf.txtExtracted Texttext/plain40667http://www.lume.ufrgs.br/bitstream/10183/185928/2/000933472.pdf.txt6f85817b39ca20d25151bed3d6193e6eMD52ORIGINAL000933472.pdfTexto completo (inglês)application/pdf1579720http://www.lume.ufrgs.br/bitstream/10183/185928/1/000933472.pdfd61f90cc091d7243b7d02617fb36ec09MD5110183/1859282019-01-17 04:25:54.736787oai:www.lume.ufrgs.br:10183/185928Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2019-01-17T06:25:54Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
spellingShingle Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
Granada, Camille Eichelberger
Rhizobium
Nodulacao
Nitrogênio
Desmodium incanum
Rhizobia
Nodulation
Nitrogen
Specificity
title_short Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_full Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_fullStr Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_full_unstemmed Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
title_sort Genetic diversity and symbiotic compatibility among rhizobial strains and Desmodium incanum and Lotus spp. plants
author Granada, Camille Eichelberger
author_facet Granada, Camille Eichelberger
Strochein, Marcos
Vargas, Luciano Kayser
Bruxel, Manuela
Sa, Enilson Luiz Saccol de
Passaglia, Luciane Maria Pereira
author_role author
author2 Strochein, Marcos
Vargas, Luciano Kayser
Bruxel, Manuela
Sa, Enilson Luiz Saccol de
Passaglia, Luciane Maria Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Granada, Camille Eichelberger
Strochein, Marcos
Vargas, Luciano Kayser
Bruxel, Manuela
Sa, Enilson Luiz Saccol de
Passaglia, Luciane Maria Pereira
dc.subject.por.fl_str_mv Rhizobium
Nodulacao
Nitrogênio
Desmodium incanum
topic Rhizobium
Nodulacao
Nitrogênio
Desmodium incanum
Rhizobia
Nodulation
Nitrogen
Specificity
dc.subject.eng.fl_str_mv Rhizobia
Nodulation
Nitrogen
Specificity
description This work aimed to evaluate the symbiotic compatibility and nodulation efficiency of rhizobia isolated from Desmodium incanum, Lotus corniculatus, L. subbiflorus, L. uliginosus and L. glaber plants by cross-inoculation. Twelve reference strains and 21 native isolates of rhizobia were genetically analyzed by the BOX-PCR technique, which showed a high genetic diversity among the rhizobia studied. The isolates were also characterized based on their production of indolic compounds and siderophores, as well as on their tolerance to salinity. Fifteen of the 33 rhizobia analyzed were able to produce indolic compounds, whereas 13 produced siderophores. All the tested rhizobia were sensitive to high salinity, although some were able to grow in solutions of up to 2% NaCl. Most of the native rhizobia isolated from L. uliginosus were able to induce nodulation in all plant species studied. In a greenhouse experiment using both D. incanum and L. corniculatus plants, the rhizobia isolate UFRGS Lu2 promoted the greatest plant growth. The results demonstrate that there are native rhizobia in the soils of southern Brazil that have low host specificity and are able to induce nodulation and form active nodules in several plant species.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2018-12-05T02:44:14Z
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Genetics and molecular biology. Ribeirão Preto, SP. Vol. 37, no. 2, (abr./jun. 2014), p. 396-405
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reponame_str Repositório Institucional da UFRGS
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