Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/250473 |
Resumo: | Aims: We hypothesized that critically ill patients with SARS-CoV-2 infection and insulin resistance would present a reduced Heat Shock Response (HSR), which is a pathway involved in proteostasis and anti-inflammation, subsequently leading to worse outcomes and higher inflammation. In this work we aimed: (i) to measure the concentration of extracellular HSP72 (eHSP72) in patients with severe COVID-19 and in comparison with noninfected patients; (ii) to compare the HSR between critically ill patients with COVID-19 (with and without diabetes); and (iii) to compare the HSR in these patients with noninfected individuals. Methods: Sixty critically ill adults with acute respiratory failure with SARS-CoV-2, with or without diabetes, were selected. Noninfected subjects were included for comparison (healthy, n = 19 and patients with diabetes, n = 22). Blood samples were collected to measure metabolism (glucose and HbA1c); oxidative stress (lypoperoxidation and carbonyls); cytokine profile (IL-10 and TNF); eHSP72; and the HSR (in vitro). Results: Patients with severe COVID-19 presented higher plasma eHSP72 compared with healthy individuals and noninfected patients with diabetes. Despite the high level of plasma cytokines, no differences were found between critically ill patients with COVID-19 with or without diabetes. Critically ill patients, when compared to noninfected, presented a blunted HSR. Oxidative stress markers followed the same pattern. No differences in the HSR (extracellular/intracellular level) were found between critically ill patients, with or without diabetes. Conclusions: We demonstrated that patients with severe COVID-19 have elevated plasma eHSP72 and that their HSR is blunted, regardless of the presence of diabetes. These results might explain the uncontrolled inflammation and also provide insights on the increased risk in developing type 2 diabetes after SARS-CoV-2 infection. |
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Russo, Mariana Kras BorgesKowalewski, Lucas StahlhöferNatividade, Gabriella Richter daMuller, Carlos Henrique de LemosSchroeder, Helena TrevisanBock, Patricia MartinsAyres, Layane RamosCardoso, Bernardo UrbanoBoeckel, Caroline Zanotto deSchein, Julia TsaoRech, Tatiana HelenaCrispim, DaisyCanani, Luis Henrique SantosFriedman, RogérioLeitão, Cristiane BauermannGerchman, FernandoKrause, Maurício da Silva2022-10-27T04:52:18Z20222218-273Xhttp://hdl.handle.net/10183/250473001151019Aims: We hypothesized that critically ill patients with SARS-CoV-2 infection and insulin resistance would present a reduced Heat Shock Response (HSR), which is a pathway involved in proteostasis and anti-inflammation, subsequently leading to worse outcomes and higher inflammation. In this work we aimed: (i) to measure the concentration of extracellular HSP72 (eHSP72) in patients with severe COVID-19 and in comparison with noninfected patients; (ii) to compare the HSR between critically ill patients with COVID-19 (with and without diabetes); and (iii) to compare the HSR in these patients with noninfected individuals. Methods: Sixty critically ill adults with acute respiratory failure with SARS-CoV-2, with or without diabetes, were selected. Noninfected subjects were included for comparison (healthy, n = 19 and patients with diabetes, n = 22). Blood samples were collected to measure metabolism (glucose and HbA1c); oxidative stress (lypoperoxidation and carbonyls); cytokine profile (IL-10 and TNF); eHSP72; and the HSR (in vitro). Results: Patients with severe COVID-19 presented higher plasma eHSP72 compared with healthy individuals and noninfected patients with diabetes. Despite the high level of plasma cytokines, no differences were found between critically ill patients with COVID-19 with or without diabetes. Critically ill patients, when compared to noninfected, presented a blunted HSR. Oxidative stress markers followed the same pattern. No differences in the HSR (extracellular/intracellular level) were found between critically ill patients, with or without diabetes. Conclusions: We demonstrated that patients with severe COVID-19 have elevated plasma eHSP72 and that their HSR is blunted, regardless of the presence of diabetes. These results might explain the uncontrolled inflammation and also provide insights on the increased risk in developing type 2 diabetes after SARS-CoV-2 infection.application/pdfengBiomolecules. Basel. Vol. 12, no. 10 (2022), artigo 1374, 16 p.COVID-19Infecções por coronavirusSARS-CoV-2InflamaçãoChoque sépticoCuidados críticosDoenças metabólicasInflammationHeat shock responseHSP72Metabolic diseasesCritically ill patientsElevated extracellular HSP72 and blunted heat shock response in severe covid-19 patientsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001151019.pdf.txt001151019.pdf.txtExtracted Texttext/plain60970http://www.lume.ufrgs.br/bitstream/10183/250473/2/001151019.pdf.txta17c96932a9ccdbfcffafb2630659ca7MD52ORIGINAL001151019.pdfTexto completo (inglês)application/pdf2939486http://www.lume.ufrgs.br/bitstream/10183/250473/1/001151019.pdf56b61264f93377200bc7ff9925c87302MD5110183/2504732023-08-09 03:49:07.091374oai:www.lume.ufrgs.br:10183/250473Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-09T06:49:07Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients |
title |
Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients |
spellingShingle |
Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients Russo, Mariana Kras Borges COVID-19 Infecções por coronavirus SARS-CoV-2 Inflamação Choque séptico Cuidados críticos Doenças metabólicas Inflammation Heat shock response HSP72 Metabolic diseases Critically ill patients |
title_short |
Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients |
title_full |
Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients |
title_fullStr |
Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients |
title_full_unstemmed |
Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients |
title_sort |
Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients |
author |
Russo, Mariana Kras Borges |
author_facet |
Russo, Mariana Kras Borges Kowalewski, Lucas Stahlhöfer Natividade, Gabriella Richter da Muller, Carlos Henrique de Lemos Schroeder, Helena Trevisan Bock, Patricia Martins Ayres, Layane Ramos Cardoso, Bernardo Urbano Boeckel, Caroline Zanotto de Schein, Julia Tsao Rech, Tatiana Helena Crispim, Daisy Canani, Luis Henrique Santos Friedman, Rogério Leitão, Cristiane Bauermann Gerchman, Fernando Krause, Maurício da Silva |
author_role |
author |
author2 |
Kowalewski, Lucas Stahlhöfer Natividade, Gabriella Richter da Muller, Carlos Henrique de Lemos Schroeder, Helena Trevisan Bock, Patricia Martins Ayres, Layane Ramos Cardoso, Bernardo Urbano Boeckel, Caroline Zanotto de Schein, Julia Tsao Rech, Tatiana Helena Crispim, Daisy Canani, Luis Henrique Santos Friedman, Rogério Leitão, Cristiane Bauermann Gerchman, Fernando Krause, Maurício da Silva |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Russo, Mariana Kras Borges Kowalewski, Lucas Stahlhöfer Natividade, Gabriella Richter da Muller, Carlos Henrique de Lemos Schroeder, Helena Trevisan Bock, Patricia Martins Ayres, Layane Ramos Cardoso, Bernardo Urbano Boeckel, Caroline Zanotto de Schein, Julia Tsao Rech, Tatiana Helena Crispim, Daisy Canani, Luis Henrique Santos Friedman, Rogério Leitão, Cristiane Bauermann Gerchman, Fernando Krause, Maurício da Silva |
dc.subject.por.fl_str_mv |
COVID-19 Infecções por coronavirus SARS-CoV-2 Inflamação Choque séptico Cuidados críticos Doenças metabólicas |
topic |
COVID-19 Infecções por coronavirus SARS-CoV-2 Inflamação Choque séptico Cuidados críticos Doenças metabólicas Inflammation Heat shock response HSP72 Metabolic diseases Critically ill patients |
dc.subject.eng.fl_str_mv |
Inflammation Heat shock response HSP72 Metabolic diseases Critically ill patients |
description |
Aims: We hypothesized that critically ill patients with SARS-CoV-2 infection and insulin resistance would present a reduced Heat Shock Response (HSR), which is a pathway involved in proteostasis and anti-inflammation, subsequently leading to worse outcomes and higher inflammation. In this work we aimed: (i) to measure the concentration of extracellular HSP72 (eHSP72) in patients with severe COVID-19 and in comparison with noninfected patients; (ii) to compare the HSR between critically ill patients with COVID-19 (with and without diabetes); and (iii) to compare the HSR in these patients with noninfected individuals. Methods: Sixty critically ill adults with acute respiratory failure with SARS-CoV-2, with or without diabetes, were selected. Noninfected subjects were included for comparison (healthy, n = 19 and patients with diabetes, n = 22). Blood samples were collected to measure metabolism (glucose and HbA1c); oxidative stress (lypoperoxidation and carbonyls); cytokine profile (IL-10 and TNF); eHSP72; and the HSR (in vitro). Results: Patients with severe COVID-19 presented higher plasma eHSP72 compared with healthy individuals and noninfected patients with diabetes. Despite the high level of plasma cytokines, no differences were found between critically ill patients with COVID-19 with or without diabetes. Critically ill patients, when compared to noninfected, presented a blunted HSR. Oxidative stress markers followed the same pattern. No differences in the HSR (extracellular/intracellular level) were found between critically ill patients, with or without diabetes. Conclusions: We demonstrated that patients with severe COVID-19 have elevated plasma eHSP72 and that their HSR is blunted, regardless of the presence of diabetes. These results might explain the uncontrolled inflammation and also provide insights on the increased risk in developing type 2 diabetes after SARS-CoV-2 infection. |
publishDate |
2022 |
dc.date.accessioned.fl_str_mv |
2022-10-27T04:52:18Z |
dc.date.issued.fl_str_mv |
2022 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/250473 |
dc.identifier.issn.pt_BR.fl_str_mv |
2218-273X |
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001151019 |
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2218-273X 001151019 |
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http://hdl.handle.net/10183/250473 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Biomolecules. Basel. Vol. 12, no. 10 (2022), artigo 1374, 16 p. |
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