Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients

Detalhes bibliográficos
Autor(a) principal: Russo, Mariana Kras Borges
Data de Publicação: 2022
Outros Autores: Kowalewski, Lucas Stahlhöfer, Natividade, Gabriella Richter da, Muller, Carlos Henrique de Lemos, Schroeder, Helena Trevisan, Bock, Patricia Martins, Ayres, Layane Ramos, Cardoso, Bernardo Urbano, Boeckel, Caroline Zanotto de, Schein, Julia Tsao, Rech, Tatiana Helena, Crispim, Daisy, Canani, Luis Henrique Santos, Friedman, Rogério, Leitão, Cristiane Bauermann, Gerchman, Fernando, Krause, Maurício da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/250473
Resumo: Aims: We hypothesized that critically ill patients with SARS-CoV-2 infection and insulin resistance would present a reduced Heat Shock Response (HSR), which is a pathway involved in proteostasis and anti-inflammation, subsequently leading to worse outcomes and higher inflammation. In this work we aimed: (i) to measure the concentration of extracellular HSP72 (eHSP72) in patients with severe COVID-19 and in comparison with noninfected patients; (ii) to compare the HSR between critically ill patients with COVID-19 (with and without diabetes); and (iii) to compare the HSR in these patients with noninfected individuals. Methods: Sixty critically ill adults with acute respiratory failure with SARS-CoV-2, with or without diabetes, were selected. Noninfected subjects were included for comparison (healthy, n = 19 and patients with diabetes, n = 22). Blood samples were collected to measure metabolism (glucose and HbA1c); oxidative stress (lypoperoxidation and carbonyls); cytokine profile (IL-10 and TNF); eHSP72; and the HSR (in vitro). Results: Patients with severe COVID-19 presented higher plasma eHSP72 compared with healthy individuals and noninfected patients with diabetes. Despite the high level of plasma cytokines, no differences were found between critically ill patients with COVID-19 with or without diabetes. Critically ill patients, when compared to noninfected, presented a blunted HSR. Oxidative stress markers followed the same pattern. No differences in the HSR (extracellular/intracellular level) were found between critically ill patients, with or without diabetes. Conclusions: We demonstrated that patients with severe COVID-19 have elevated plasma eHSP72 and that their HSR is blunted, regardless of the presence of diabetes. These results might explain the uncontrolled inflammation and also provide insights on the increased risk in developing type 2 diabetes after SARS-CoV-2 infection.
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spelling Russo, Mariana Kras BorgesKowalewski, Lucas StahlhöferNatividade, Gabriella Richter daMuller, Carlos Henrique de LemosSchroeder, Helena TrevisanBock, Patricia MartinsAyres, Layane RamosCardoso, Bernardo UrbanoBoeckel, Caroline Zanotto deSchein, Julia TsaoRech, Tatiana HelenaCrispim, DaisyCanani, Luis Henrique SantosFriedman, RogérioLeitão, Cristiane BauermannGerchman, FernandoKrause, Maurício da Silva2022-10-27T04:52:18Z20222218-273Xhttp://hdl.handle.net/10183/250473001151019Aims: We hypothesized that critically ill patients with SARS-CoV-2 infection and insulin resistance would present a reduced Heat Shock Response (HSR), which is a pathway involved in proteostasis and anti-inflammation, subsequently leading to worse outcomes and higher inflammation. In this work we aimed: (i) to measure the concentration of extracellular HSP72 (eHSP72) in patients with severe COVID-19 and in comparison with noninfected patients; (ii) to compare the HSR between critically ill patients with COVID-19 (with and without diabetes); and (iii) to compare the HSR in these patients with noninfected individuals. Methods: Sixty critically ill adults with acute respiratory failure with SARS-CoV-2, with or without diabetes, were selected. Noninfected subjects were included for comparison (healthy, n = 19 and patients with diabetes, n = 22). Blood samples were collected to measure metabolism (glucose and HbA1c); oxidative stress (lypoperoxidation and carbonyls); cytokine profile (IL-10 and TNF); eHSP72; and the HSR (in vitro). Results: Patients with severe COVID-19 presented higher plasma eHSP72 compared with healthy individuals and noninfected patients with diabetes. Despite the high level of plasma cytokines, no differences were found between critically ill patients with COVID-19 with or without diabetes. Critically ill patients, when compared to noninfected, presented a blunted HSR. Oxidative stress markers followed the same pattern. No differences in the HSR (extracellular/intracellular level) were found between critically ill patients, with or without diabetes. Conclusions: We demonstrated that patients with severe COVID-19 have elevated plasma eHSP72 and that their HSR is blunted, regardless of the presence of diabetes. These results might explain the uncontrolled inflammation and also provide insights on the increased risk in developing type 2 diabetes after SARS-CoV-2 infection.application/pdfengBiomolecules. Basel. Vol. 12, no. 10 (2022), artigo 1374, 16 p.COVID-19Infecções por coronavirusSARS-CoV-2InflamaçãoChoque sépticoCuidados críticosDoenças metabólicasInflammationHeat shock responseHSP72Metabolic diseasesCritically ill patientsElevated extracellular HSP72 and blunted heat shock response in severe covid-19 patientsEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001151019.pdf.txt001151019.pdf.txtExtracted Texttext/plain60970http://www.lume.ufrgs.br/bitstream/10183/250473/2/001151019.pdf.txta17c96932a9ccdbfcffafb2630659ca7MD52ORIGINAL001151019.pdfTexto completo (inglês)application/pdf2939486http://www.lume.ufrgs.br/bitstream/10183/250473/1/001151019.pdf56b61264f93377200bc7ff9925c87302MD5110183/2504732023-08-09 03:49:07.091374oai:www.lume.ufrgs.br:10183/250473Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-08-09T06:49:07Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
title Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
spellingShingle Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
Russo, Mariana Kras Borges
COVID-19
Infecções por coronavirus
SARS-CoV-2
Inflamação
Choque séptico
Cuidados críticos
Doenças metabólicas
Inflammation
Heat shock response
HSP72
Metabolic diseases
Critically ill patients
title_short Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
title_full Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
title_fullStr Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
title_full_unstemmed Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
title_sort Elevated extracellular HSP72 and blunted heat shock response in severe covid-19 patients
author Russo, Mariana Kras Borges
author_facet Russo, Mariana Kras Borges
Kowalewski, Lucas Stahlhöfer
Natividade, Gabriella Richter da
Muller, Carlos Henrique de Lemos
Schroeder, Helena Trevisan
Bock, Patricia Martins
Ayres, Layane Ramos
Cardoso, Bernardo Urbano
Boeckel, Caroline Zanotto de
Schein, Julia Tsao
Rech, Tatiana Helena
Crispim, Daisy
Canani, Luis Henrique Santos
Friedman, Rogério
Leitão, Cristiane Bauermann
Gerchman, Fernando
Krause, Maurício da Silva
author_role author
author2 Kowalewski, Lucas Stahlhöfer
Natividade, Gabriella Richter da
Muller, Carlos Henrique de Lemos
Schroeder, Helena Trevisan
Bock, Patricia Martins
Ayres, Layane Ramos
Cardoso, Bernardo Urbano
Boeckel, Caroline Zanotto de
Schein, Julia Tsao
Rech, Tatiana Helena
Crispim, Daisy
Canani, Luis Henrique Santos
Friedman, Rogério
Leitão, Cristiane Bauermann
Gerchman, Fernando
Krause, Maurício da Silva
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Russo, Mariana Kras Borges
Kowalewski, Lucas Stahlhöfer
Natividade, Gabriella Richter da
Muller, Carlos Henrique de Lemos
Schroeder, Helena Trevisan
Bock, Patricia Martins
Ayres, Layane Ramos
Cardoso, Bernardo Urbano
Boeckel, Caroline Zanotto de
Schein, Julia Tsao
Rech, Tatiana Helena
Crispim, Daisy
Canani, Luis Henrique Santos
Friedman, Rogério
Leitão, Cristiane Bauermann
Gerchman, Fernando
Krause, Maurício da Silva
dc.subject.por.fl_str_mv COVID-19
Infecções por coronavirus
SARS-CoV-2
Inflamação
Choque séptico
Cuidados críticos
Doenças metabólicas
topic COVID-19
Infecções por coronavirus
SARS-CoV-2
Inflamação
Choque séptico
Cuidados críticos
Doenças metabólicas
Inflammation
Heat shock response
HSP72
Metabolic diseases
Critically ill patients
dc.subject.eng.fl_str_mv Inflammation
Heat shock response
HSP72
Metabolic diseases
Critically ill patients
description Aims: We hypothesized that critically ill patients with SARS-CoV-2 infection and insulin resistance would present a reduced Heat Shock Response (HSR), which is a pathway involved in proteostasis and anti-inflammation, subsequently leading to worse outcomes and higher inflammation. In this work we aimed: (i) to measure the concentration of extracellular HSP72 (eHSP72) in patients with severe COVID-19 and in comparison with noninfected patients; (ii) to compare the HSR between critically ill patients with COVID-19 (with and without diabetes); and (iii) to compare the HSR in these patients with noninfected individuals. Methods: Sixty critically ill adults with acute respiratory failure with SARS-CoV-2, with or without diabetes, were selected. Noninfected subjects were included for comparison (healthy, n = 19 and patients with diabetes, n = 22). Blood samples were collected to measure metabolism (glucose and HbA1c); oxidative stress (lypoperoxidation and carbonyls); cytokine profile (IL-10 and TNF); eHSP72; and the HSR (in vitro). Results: Patients with severe COVID-19 presented higher plasma eHSP72 compared with healthy individuals and noninfected patients with diabetes. Despite the high level of plasma cytokines, no differences were found between critically ill patients with COVID-19 with or without diabetes. Critically ill patients, when compared to noninfected, presented a blunted HSR. Oxidative stress markers followed the same pattern. No differences in the HSR (extracellular/intracellular level) were found between critically ill patients, with or without diabetes. Conclusions: We demonstrated that patients with severe COVID-19 have elevated plasma eHSP72 and that their HSR is blunted, regardless of the presence of diabetes. These results might explain the uncontrolled inflammation and also provide insights on the increased risk in developing type 2 diabetes after SARS-CoV-2 infection.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-10-27T04:52:18Z
dc.date.issued.fl_str_mv 2022
dc.type.driver.fl_str_mv Estrangeiro
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Biomolecules. Basel. Vol. 12, no. 10 (2022), artigo 1374, 16 p.
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