Transforming growth factor-beta family members are regulated during induced luteolysis in cattle
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/224311 |
Resumo: | The transforming growth factors beta (TGFβ) are local factors produced by ovarian cells which, after binding to their receptors, regulate follicular deviation and ovulation. However, their regulation and function during corpus luteum (CL) regression has been poorly investigated. The present study evaluated the mRNA regulation of some TGFβ family ligands and their receptors in the bovine CL during induced luteolysis in vivo. On day 10 of the estrous cycle, cows received an injection of prostaglandin F2α (PGF) and luteal samples were obtained from separate groups of cows (n= 4-5 cows per time-point) at 0, 2, 12, 24 or 48 h after treatment. Since TGF beta family comprises more than 30 ligands, we focused in some candidates genes such as activin receptors (ACVR-1A, -1B, -2A, -2B) AMH, AMHR2, BMPs (BMP-1, -2, -3, -4, -6 and -7), BMP receptors (BMPR 1A, -1B and -2), inhibin subunits (INH-A, -BA, -BB) and betaglycan (TGFBR3). The mRNA levels of BMP4, BMP6 and INHBA were higher at 2 h after PGF administration (P<0.05) in comparison to 0 h. The relative mRNA abundance of BMP1, BMP2, BMP3, BMP4, BMP6, ACVR1B, INHBA and INHBB was upregulated up to 12 h post PGF (P<0.05). On the other hand, TGFBR3 mRNA that codes for a reservoir of ligands that bind to TGF-beta receptors, was lower at 48 h. In conclusion, findings from this study demonstrated that genes encoding several TGFβ family members are expressed in a time-specific manner after PGF administration. |
id |
UFRGS-2_a21b867a03671c744867ff06e4f1d277 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/224311 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Haas, Cristina SangoiRovani, Monique TomazeleIlha, Gustavo FreitasBertolin, KalyneFerst, Juliana GermanoBridi, AlessandraBordignon, VilceuDuggavathi, RajAntoniazzi, Alfredo QuitesGoncalves, Paulo Bayard DiasGasperin, Bernardo Garziera2021-07-21T04:24:42Z20191806-9614http://hdl.handle.net/10183/224311001127449The transforming growth factors beta (TGFβ) are local factors produced by ovarian cells which, after binding to their receptors, regulate follicular deviation and ovulation. However, their regulation and function during corpus luteum (CL) regression has been poorly investigated. The present study evaluated the mRNA regulation of some TGFβ family ligands and their receptors in the bovine CL during induced luteolysis in vivo. On day 10 of the estrous cycle, cows received an injection of prostaglandin F2α (PGF) and luteal samples were obtained from separate groups of cows (n= 4-5 cows per time-point) at 0, 2, 12, 24 or 48 h after treatment. Since TGF beta family comprises more than 30 ligands, we focused in some candidates genes such as activin receptors (ACVR-1A, -1B, -2A, -2B) AMH, AMHR2, BMPs (BMP-1, -2, -3, -4, -6 and -7), BMP receptors (BMPR 1A, -1B and -2), inhibin subunits (INH-A, -BA, -BB) and betaglycan (TGFBR3). The mRNA levels of BMP4, BMP6 and INHBA were higher at 2 h after PGF administration (P<0.05) in comparison to 0 h. The relative mRNA abundance of BMP1, BMP2, BMP3, BMP4, BMP6, ACVR1B, INHBA and INHBB was upregulated up to 12 h post PGF (P<0.05). On the other hand, TGFBR3 mRNA that codes for a reservoir of ligands that bind to TGF-beta receptors, was lower at 48 h. In conclusion, findings from this study demonstrated that genes encoding several TGFβ family members are expressed in a time-specific manner after PGF administration.application/pdfengAnimal reproduction. Belo Horizonte. Vol. 16, n. 4 (2019), p. 829-837Fator de crescimento transformador BetaReceptores de fatores de crescimento transformadores betaCorpo lúteoLuteóliseBovinosCorpus luteumLuteolysisCattleTransforming growth factor-beta family members are regulated during induced luteolysis in cattleinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001127449.pdf.txt001127449.pdf.txtExtracted Texttext/plain40378http://www.lume.ufrgs.br/bitstream/10183/224311/2/001127449.pdf.txtec2b80c4bc1aabe5f6d04f81a5ede170MD52ORIGINAL001127449.pdfTexto completo (inglês)application/pdf4983180http://www.lume.ufrgs.br/bitstream/10183/224311/1/001127449.pdf0980394b5550b6bd2f5a957a7095ac12MD5110183/2243112021-08-18 04:50:51.145568oai:www.lume.ufrgs.br:10183/224311Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-08-18T07:50:51Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Transforming growth factor-beta family members are regulated during induced luteolysis in cattle |
title |
Transforming growth factor-beta family members are regulated during induced luteolysis in cattle |
spellingShingle |
Transforming growth factor-beta family members are regulated during induced luteolysis in cattle Haas, Cristina Sangoi Fator de crescimento transformador Beta Receptores de fatores de crescimento transformadores beta Corpo lúteo Luteólise Bovinos Corpus luteum Luteolysis Cattle |
title_short |
Transforming growth factor-beta family members are regulated during induced luteolysis in cattle |
title_full |
Transforming growth factor-beta family members are regulated during induced luteolysis in cattle |
title_fullStr |
Transforming growth factor-beta family members are regulated during induced luteolysis in cattle |
title_full_unstemmed |
Transforming growth factor-beta family members are regulated during induced luteolysis in cattle |
title_sort |
Transforming growth factor-beta family members are regulated during induced luteolysis in cattle |
author |
Haas, Cristina Sangoi |
author_facet |
Haas, Cristina Sangoi Rovani, Monique Tomazele Ilha, Gustavo Freitas Bertolin, Kalyne Ferst, Juliana Germano Bridi, Alessandra Bordignon, Vilceu Duggavathi, Raj Antoniazzi, Alfredo Quites Goncalves, Paulo Bayard Dias Gasperin, Bernardo Garziera |
author_role |
author |
author2 |
Rovani, Monique Tomazele Ilha, Gustavo Freitas Bertolin, Kalyne Ferst, Juliana Germano Bridi, Alessandra Bordignon, Vilceu Duggavathi, Raj Antoniazzi, Alfredo Quites Goncalves, Paulo Bayard Dias Gasperin, Bernardo Garziera |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Haas, Cristina Sangoi Rovani, Monique Tomazele Ilha, Gustavo Freitas Bertolin, Kalyne Ferst, Juliana Germano Bridi, Alessandra Bordignon, Vilceu Duggavathi, Raj Antoniazzi, Alfredo Quites Goncalves, Paulo Bayard Dias Gasperin, Bernardo Garziera |
dc.subject.por.fl_str_mv |
Fator de crescimento transformador Beta Receptores de fatores de crescimento transformadores beta Corpo lúteo Luteólise Bovinos |
topic |
Fator de crescimento transformador Beta Receptores de fatores de crescimento transformadores beta Corpo lúteo Luteólise Bovinos Corpus luteum Luteolysis Cattle |
dc.subject.eng.fl_str_mv |
Corpus luteum Luteolysis Cattle |
description |
The transforming growth factors beta (TGFβ) are local factors produced by ovarian cells which, after binding to their receptors, regulate follicular deviation and ovulation. However, their regulation and function during corpus luteum (CL) regression has been poorly investigated. The present study evaluated the mRNA regulation of some TGFβ family ligands and their receptors in the bovine CL during induced luteolysis in vivo. On day 10 of the estrous cycle, cows received an injection of prostaglandin F2α (PGF) and luteal samples were obtained from separate groups of cows (n= 4-5 cows per time-point) at 0, 2, 12, 24 or 48 h after treatment. Since TGF beta family comprises more than 30 ligands, we focused in some candidates genes such as activin receptors (ACVR-1A, -1B, -2A, -2B) AMH, AMHR2, BMPs (BMP-1, -2, -3, -4, -6 and -7), BMP receptors (BMPR 1A, -1B and -2), inhibin subunits (INH-A, -BA, -BB) and betaglycan (TGFBR3). The mRNA levels of BMP4, BMP6 and INHBA were higher at 2 h after PGF administration (P<0.05) in comparison to 0 h. The relative mRNA abundance of BMP1, BMP2, BMP3, BMP4, BMP6, ACVR1B, INHBA and INHBB was upregulated up to 12 h post PGF (P<0.05). On the other hand, TGFBR3 mRNA that codes for a reservoir of ligands that bind to TGF-beta receptors, was lower at 48 h. In conclusion, findings from this study demonstrated that genes encoding several TGFβ family members are expressed in a time-specific manner after PGF administration. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2021-07-21T04:24:42Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/224311 |
dc.identifier.issn.pt_BR.fl_str_mv |
1806-9614 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001127449 |
identifier_str_mv |
1806-9614 001127449 |
url |
http://hdl.handle.net/10183/224311 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Animal reproduction. Belo Horizonte. Vol. 16, n. 4 (2019), p. 829-837 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/224311/2/001127449.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/224311/1/001127449.pdf |
bitstream.checksum.fl_str_mv |
ec2b80c4bc1aabe5f6d04f81a5ede170 0980394b5550b6bd2f5a957a7095ac12 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225027105849344 |