Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector

Detalhes bibliográficos
Autor(a) principal: Hoeltz, Michele
Data de Publicação: 2010
Outros Autores: Welke, Juliane Elisa, Noll, Isa Beatriz, Dottori, Horacio Alberto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/72879
Resumo: A photometric procedure was developed for determination of aflatoxin B1 in peanuts by TLC-CCD technique. The quantification and detection limit were 1.2 μg kg-1 and 0.4 ng per spot, respectively, with mean recovery of 98%. The CCD camera is sufficiently sensitive to detect small changes in spots fluorescence intensity and the results for performance confirmed the efficiency of the method. Another important property of CCD detector is its linearity for a wide range of luminous stimulus determined by analysis of five-point calibration curves using the intensity of AFB1 fluorescence versus AFB1 concentration (0.8 to 4.8 ng per spot). The method was applied to the analysis of thirty nine peanut samples and aflatoxin B1 levels ranged from 16 to 115 μg kg-1. The TLC-CCD and the photometric procedure developed in this study demonstrated to be a simple and efficient tool for quantitative analyses of AFB1 in peanut samples.
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spelling Hoeltz, MicheleWelke, Juliane ElisaNoll, Isa BeatrizDottori, Horacio Alberto2013-07-02T01:43:19Z20100100-4042http://hdl.handle.net/10183/72879000733411A photometric procedure was developed for determination of aflatoxin B1 in peanuts by TLC-CCD technique. The quantification and detection limit were 1.2 μg kg-1 and 0.4 ng per spot, respectively, with mean recovery of 98%. The CCD camera is sufficiently sensitive to detect small changes in spots fluorescence intensity and the results for performance confirmed the efficiency of the method. Another important property of CCD detector is its linearity for a wide range of luminous stimulus determined by analysis of five-point calibration curves using the intensity of AFB1 fluorescence versus AFB1 concentration (0.8 to 4.8 ng per spot). The method was applied to the analysis of thirty nine peanut samples and aflatoxin B1 levels ranged from 16 to 115 μg kg-1. The TLC-CCD and the photometric procedure developed in this study demonstrated to be a simple and efficient tool for quantitative analyses of AFB1 in peanut samples.application/pdfporQuímica nova. São Paulo. Vol. 33, n. 1 (2010), p. 43-47Amendoim : AflatoxinaAmendoimAflatoxina B1Aflatoxin B1Photometric procedureTLC-CCDPhotometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detectorinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000733411.pdf.txt000733411.pdf.txtExtracted Texttext/plain25011http://www.lume.ufrgs.br/bitstream/10183/72879/2/000733411.pdf.txt328f03a057b26d9932565d978498e0f7MD52ORIGINAL000733411.pdf000733411.pdfTexto completoapplication/pdf414880http://www.lume.ufrgs.br/bitstream/10183/72879/1/000733411.pdfdb8bf82c7189645b126db896b47ea19cMD51THUMBNAIL000733411.pdf.jpg000733411.pdf.jpgGenerated Thumbnailimage/jpeg1940http://www.lume.ufrgs.br/bitstream/10183/72879/3/000733411.pdf.jpgcf88e139ce164e1a348dbe91840a6e1eMD5310183/728792024-01-31 05:59:25.869829oai:www.lume.ufrgs.br:10183/72879Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-01-31T07:59:25Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector
title Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector
spellingShingle Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector
Hoeltz, Michele
Amendoim : Aflatoxina
Amendoim
Aflatoxina B1
Aflatoxin B1
Photometric procedure
TLC-CCD
title_short Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector
title_full Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector
title_fullStr Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector
title_full_unstemmed Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector
title_sort Photometric procedure for quantitative analysis of aflatoxin b1 in peanuts by thin-layer chromatography using charge coupled device detector
author Hoeltz, Michele
author_facet Hoeltz, Michele
Welke, Juliane Elisa
Noll, Isa Beatriz
Dottori, Horacio Alberto
author_role author
author2 Welke, Juliane Elisa
Noll, Isa Beatriz
Dottori, Horacio Alberto
author2_role author
author
author
dc.contributor.author.fl_str_mv Hoeltz, Michele
Welke, Juliane Elisa
Noll, Isa Beatriz
Dottori, Horacio Alberto
dc.subject.por.fl_str_mv Amendoim : Aflatoxina
Amendoim
Aflatoxina B1
topic Amendoim : Aflatoxina
Amendoim
Aflatoxina B1
Aflatoxin B1
Photometric procedure
TLC-CCD
dc.subject.eng.fl_str_mv Aflatoxin B1
Photometric procedure
TLC-CCD
description A photometric procedure was developed for determination of aflatoxin B1 in peanuts by TLC-CCD technique. The quantification and detection limit were 1.2 μg kg-1 and 0.4 ng per spot, respectively, with mean recovery of 98%. The CCD camera is sufficiently sensitive to detect small changes in spots fluorescence intensity and the results for performance confirmed the efficiency of the method. Another important property of CCD detector is its linearity for a wide range of luminous stimulus determined by analysis of five-point calibration curves using the intensity of AFB1 fluorescence versus AFB1 concentration (0.8 to 4.8 ng per spot). The method was applied to the analysis of thirty nine peanut samples and aflatoxin B1 levels ranged from 16 to 115 μg kg-1. The TLC-CCD and the photometric procedure developed in this study demonstrated to be a simple and efficient tool for quantitative analyses of AFB1 in peanut samples.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2013-07-02T01:43:19Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/72879
dc.identifier.issn.pt_BR.fl_str_mv 0100-4042
dc.identifier.nrb.pt_BR.fl_str_mv 000733411
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url http://hdl.handle.net/10183/72879
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dc.relation.ispartof.pt_BR.fl_str_mv Química nova. São Paulo. Vol. 33, n. 1 (2010), p. 43-47
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