Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/182934 |
Resumo: | Dermatomycosis causes highly frequent dermal lesions, and volatile oils have been proven to be promising as antifungal agents. The antifungal activity of geraniol, nerol, citral, neral and geranial (monoterpenes), and terbinafine and anidulafungin (control drugs) against seven opportunistic pathogenic yeasts and four dermatophyte species was evaluated by the Clinical and Laboratory Standards Institute microdilution tests. Monoterpenes were more active against dermatophytes than yeasts (geometric mean of minimal inhibitory concentration (GMIC) of 34.5 and 100.4 μg.ml-1, respectively). Trichophyton rubrum was the fungal species most sensitive to monoterpenes (GMIC of 22.9 μg.ml-1). The trans isomers showed higher antifungal activity than the cis. The mechanism of action was investigated evaluating damage in the fungal cell wall (Sorbitol Protection Assay) and in the cell membrane (Ergosterol Affinity Assay). No changes were observed in the MIC of monoterpenes in the sorbitol protection assay. The MIC of citral and geraniol was increased from 32 to 160 μg.ml-1 when the exogenous ergosterol concentrations was zero and 250 μg.ml-1, respectively. The monoterpenes showed an affinity for ergosterol relating their mechanism of action to cell membrane destabilization. |
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Miron, Diogo dos SantosBattisti, FernandaSilva, Fernanda Kraemer daLana, Aline D.Pippi, BrunaCasanova, Bruna BentoGnoatto, Simone Cristina BaggioFuentefria, Alexandre MeneghelloMayorga, PauloSchapoval, Elfrides Eva Scherman2018-09-28T02:35:06Z20140102-695Xhttp://hdl.handle.net/10183/182934000955219Dermatomycosis causes highly frequent dermal lesions, and volatile oils have been proven to be promising as antifungal agents. The antifungal activity of geraniol, nerol, citral, neral and geranial (monoterpenes), and terbinafine and anidulafungin (control drugs) against seven opportunistic pathogenic yeasts and four dermatophyte species was evaluated by the Clinical and Laboratory Standards Institute microdilution tests. Monoterpenes were more active against dermatophytes than yeasts (geometric mean of minimal inhibitory concentration (GMIC) of 34.5 and 100.4 μg.ml-1, respectively). Trichophyton rubrum was the fungal species most sensitive to monoterpenes (GMIC of 22.9 μg.ml-1). The trans isomers showed higher antifungal activity than the cis. The mechanism of action was investigated evaluating damage in the fungal cell wall (Sorbitol Protection Assay) and in the cell membrane (Ergosterol Affinity Assay). No changes were observed in the MIC of monoterpenes in the sorbitol protection assay. The MIC of citral and geraniol was increased from 32 to 160 μg.ml-1 when the exogenous ergosterol concentrations was zero and 250 μg.ml-1, respectively. The monoterpenes showed an affinity for ergosterol relating their mechanism of action to cell membrane destabilization.application/pdfengRevista brasileira de farmacognosia. Vol. 24, n. 6 (Out/Dez 2014), p. 660-667FarmáciaCitralGeraniolNerolIsomersMicrodilution assayMechanism of actionAntifungal activity and mechanism of action of monoterpenes against dermatophytes and yeastsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000955219.pdfTexto completo (inglês)application/pdf1122245http://www.lume.ufrgs.br/bitstream/10183/182934/1/000955219.pdfc3b1814342982a9203af407f462546c1MD51TEXT000955219.pdf.txt000955219.pdf.txtExtracted Texttext/plain32319http://www.lume.ufrgs.br/bitstream/10183/182934/2/000955219.pdf.txt446b928bf7a87b2b3d60688a76ca7f38MD52THUMBNAIL000955219.pdf.jpg000955219.pdf.jpgGenerated Thumbnailimage/jpeg2036http://www.lume.ufrgs.br/bitstream/10183/182934/3/000955219.pdf.jpgd7b88f365fb7ccbe4464f33197e20418MD5310183/1829342018-10-05 08:01:29.615oai:www.lume.ufrgs.br:10183/182934Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-05T11:01:29Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts |
title |
Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts |
spellingShingle |
Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts Miron, Diogo dos Santos Farmácia Citral Geraniol Nerol Isomers Microdilution assay Mechanism of action |
title_short |
Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts |
title_full |
Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts |
title_fullStr |
Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts |
title_full_unstemmed |
Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts |
title_sort |
Antifungal activity and mechanism of action of monoterpenes against dermatophytes and yeasts |
author |
Miron, Diogo dos Santos |
author_facet |
Miron, Diogo dos Santos Battisti, Fernanda Silva, Fernanda Kraemer da Lana, Aline D. Pippi, Bruna Casanova, Bruna Bento Gnoatto, Simone Cristina Baggio Fuentefria, Alexandre Meneghello Mayorga, Paulo Schapoval, Elfrides Eva Scherman |
author_role |
author |
author2 |
Battisti, Fernanda Silva, Fernanda Kraemer da Lana, Aline D. Pippi, Bruna Casanova, Bruna Bento Gnoatto, Simone Cristina Baggio Fuentefria, Alexandre Meneghello Mayorga, Paulo Schapoval, Elfrides Eva Scherman |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Miron, Diogo dos Santos Battisti, Fernanda Silva, Fernanda Kraemer da Lana, Aline D. Pippi, Bruna Casanova, Bruna Bento Gnoatto, Simone Cristina Baggio Fuentefria, Alexandre Meneghello Mayorga, Paulo Schapoval, Elfrides Eva Scherman |
dc.subject.por.fl_str_mv |
Farmácia |
topic |
Farmácia Citral Geraniol Nerol Isomers Microdilution assay Mechanism of action |
dc.subject.eng.fl_str_mv |
Citral Geraniol Nerol Isomers Microdilution assay Mechanism of action |
description |
Dermatomycosis causes highly frequent dermal lesions, and volatile oils have been proven to be promising as antifungal agents. The antifungal activity of geraniol, nerol, citral, neral and geranial (monoterpenes), and terbinafine and anidulafungin (control drugs) against seven opportunistic pathogenic yeasts and four dermatophyte species was evaluated by the Clinical and Laboratory Standards Institute microdilution tests. Monoterpenes were more active against dermatophytes than yeasts (geometric mean of minimal inhibitory concentration (GMIC) of 34.5 and 100.4 μg.ml-1, respectively). Trichophyton rubrum was the fungal species most sensitive to monoterpenes (GMIC of 22.9 μg.ml-1). The trans isomers showed higher antifungal activity than the cis. The mechanism of action was investigated evaluating damage in the fungal cell wall (Sorbitol Protection Assay) and in the cell membrane (Ergosterol Affinity Assay). No changes were observed in the MIC of monoterpenes in the sorbitol protection assay. The MIC of citral and geraniol was increased from 32 to 160 μg.ml-1 when the exogenous ergosterol concentrations was zero and 250 μg.ml-1, respectively. The monoterpenes showed an affinity for ergosterol relating their mechanism of action to cell membrane destabilization. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2018-09-28T02:35:06Z |
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publishedVersion |
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http://hdl.handle.net/10183/182934 |
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0102-695X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000955219 |
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http://hdl.handle.net/10183/182934 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista brasileira de farmacognosia. Vol. 24, n. 6 (Out/Dez 2014), p. 660-667 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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