Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing
Autor(a) principal: | |
---|---|
Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/165870 |
Resumo: | Phenylketonuria (PKU) is an inborn error of metabolism associated with high blood levels of phenylalanine (Phe). A Phe-restricted diet supplemented with L-amino acids is the main treatment strategy for this disease; if started early, most neurological abnormalities can be prevented. The healthy human gut contains trillions of commensal bacteria, often referred to as the gut microbiota. The composition of the gut microbiota is known to be modulated by environmental factors, including diet. In this study, we compared the gut microbiota of 8 PKU patients on Phe-restricted dietary treatment with that of 10 healthy individuals. The microbiota were characterized by 16S rRNA sequencing using the Ion Torrent™platform. The most dominant phyla detected in both groups were Bacteroidetes and Firmicutes. PKU patients showed reduced abundance of the Clostridiaceae, Erysipelotrichaceae, and Lachnospiraceae families, Clostridiales class, Coprococcus, Dorea, Lachnospira, Odoribacter, Ruminococcus and Veillonella genera, and enrichment of Prevotella, Akkermansia, and Peptostreptococcaceae. Microbial function prediction suggested significant differences in starch/glucose and amino acid metabolism between PKU patients and controls. Together, our results suggest the presence of distinct taxonomic groups within the gut microbiome of PKU patients, which may be modulated by their plasma Phe concentration. Whether our findings represent an effect of the disease itself, or a consequence of the modified diet is unclear. |
id |
UFRGS-2_a841bf6f53fa5ce726c07a0fabd43f63 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/165870 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Oliveira, Felipe Pinheiro deMendes, Roberta HackDobbler, Priscila Caroline ThiagoMai, VolkerPylro, Victor SatlerWaugh, Sheldon G.Vairo, Filippo Pinto eRefosco, Lilia FarretRoesch, Luiz Fernando WurdigSchwartz, Ida Vanessa Doederlein2017-08-29T02:33:54Z20161932-6203http://hdl.handle.net/10183/165870001044741Phenylketonuria (PKU) is an inborn error of metabolism associated with high blood levels of phenylalanine (Phe). A Phe-restricted diet supplemented with L-amino acids is the main treatment strategy for this disease; if started early, most neurological abnormalities can be prevented. The healthy human gut contains trillions of commensal bacteria, often referred to as the gut microbiota. The composition of the gut microbiota is known to be modulated by environmental factors, including diet. In this study, we compared the gut microbiota of 8 PKU patients on Phe-restricted dietary treatment with that of 10 healthy individuals. The microbiota were characterized by 16S rRNA sequencing using the Ion Torrent™platform. The most dominant phyla detected in both groups were Bacteroidetes and Firmicutes. PKU patients showed reduced abundance of the Clostridiaceae, Erysipelotrichaceae, and Lachnospiraceae families, Clostridiales class, Coprococcus, Dorea, Lachnospira, Odoribacter, Ruminococcus and Veillonella genera, and enrichment of Prevotella, Akkermansia, and Peptostreptococcaceae. Microbial function prediction suggested significant differences in starch/glucose and amino acid metabolism between PKU patients and controls. Together, our results suggest the presence of distinct taxonomic groups within the gut microbiome of PKU patients, which may be modulated by their plasma Phe concentration. Whether our findings represent an effect of the disease itself, or a consequence of the modified diet is unclear.application/pdfengPLoS ONE. San Francisco. Vol. 11, no. 6 (June 2016), e0157513, 15 p.Microbioma gastrointestinalSequenciamento de nucleotídeos em larga escalaMetagenômicaFenilcetonúriasRNA ribossômico 16SPhenylketonuria and gut microbiota : a controlled study based on next-generation sequencingEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001044741.pdf001044741.pdfTexto completo (inglês)application/pdf1534191http://www.lume.ufrgs.br/bitstream/10183/165870/1/001044741.pdf96d5028136cf9551de431ce6bb954ceaMD51TEXT001044741.pdf.txt001044741.pdf.txtExtracted Texttext/plain49818http://www.lume.ufrgs.br/bitstream/10183/165870/2/001044741.pdf.txt3046ada9d290b736928d565560206821MD52THUMBNAIL001044741.pdf.jpg001044741.pdf.jpgGenerated Thumbnailimage/jpeg1889http://www.lume.ufrgs.br/bitstream/10183/165870/3/001044741.pdf.jpgca9ca38dfce6ede02a884a104bf964deMD5310183/1658702023-05-25 03:25:29.005056oai:www.lume.ufrgs.br:10183/165870Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2023-05-25T06:25:29Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing |
title |
Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing |
spellingShingle |
Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing Oliveira, Felipe Pinheiro de Microbioma gastrointestinal Sequenciamento de nucleotídeos em larga escala Metagenômica Fenilcetonúrias RNA ribossômico 16S |
title_short |
Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing |
title_full |
Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing |
title_fullStr |
Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing |
title_full_unstemmed |
Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing |
title_sort |
Phenylketonuria and gut microbiota : a controlled study based on next-generation sequencing |
author |
Oliveira, Felipe Pinheiro de |
author_facet |
Oliveira, Felipe Pinheiro de Mendes, Roberta Hack Dobbler, Priscila Caroline Thiago Mai, Volker Pylro, Victor Satler Waugh, Sheldon G. Vairo, Filippo Pinto e Refosco, Lilia Farret Roesch, Luiz Fernando Wurdig Schwartz, Ida Vanessa Doederlein |
author_role |
author |
author2 |
Mendes, Roberta Hack Dobbler, Priscila Caroline Thiago Mai, Volker Pylro, Victor Satler Waugh, Sheldon G. Vairo, Filippo Pinto e Refosco, Lilia Farret Roesch, Luiz Fernando Wurdig Schwartz, Ida Vanessa Doederlein |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Felipe Pinheiro de Mendes, Roberta Hack Dobbler, Priscila Caroline Thiago Mai, Volker Pylro, Victor Satler Waugh, Sheldon G. Vairo, Filippo Pinto e Refosco, Lilia Farret Roesch, Luiz Fernando Wurdig Schwartz, Ida Vanessa Doederlein |
dc.subject.por.fl_str_mv |
Microbioma gastrointestinal Sequenciamento de nucleotídeos em larga escala Metagenômica Fenilcetonúrias RNA ribossômico 16S |
topic |
Microbioma gastrointestinal Sequenciamento de nucleotídeos em larga escala Metagenômica Fenilcetonúrias RNA ribossômico 16S |
description |
Phenylketonuria (PKU) is an inborn error of metabolism associated with high blood levels of phenylalanine (Phe). A Phe-restricted diet supplemented with L-amino acids is the main treatment strategy for this disease; if started early, most neurological abnormalities can be prevented. The healthy human gut contains trillions of commensal bacteria, often referred to as the gut microbiota. The composition of the gut microbiota is known to be modulated by environmental factors, including diet. In this study, we compared the gut microbiota of 8 PKU patients on Phe-restricted dietary treatment with that of 10 healthy individuals. The microbiota were characterized by 16S rRNA sequencing using the Ion Torrent™platform. The most dominant phyla detected in both groups were Bacteroidetes and Firmicutes. PKU patients showed reduced abundance of the Clostridiaceae, Erysipelotrichaceae, and Lachnospiraceae families, Clostridiales class, Coprococcus, Dorea, Lachnospira, Odoribacter, Ruminococcus and Veillonella genera, and enrichment of Prevotella, Akkermansia, and Peptostreptococcaceae. Microbial function prediction suggested significant differences in starch/glucose and amino acid metabolism between PKU patients and controls. Together, our results suggest the presence of distinct taxonomic groups within the gut microbiome of PKU patients, which may be modulated by their plasma Phe concentration. Whether our findings represent an effect of the disease itself, or a consequence of the modified diet is unclear. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016 |
dc.date.accessioned.fl_str_mv |
2017-08-29T02:33:54Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/165870 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001044741 |
identifier_str_mv |
1932-6203 001044741 |
url |
http://hdl.handle.net/10183/165870 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE. San Francisco. Vol. 11, no. 6 (June 2016), e0157513, 15 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/165870/1/001044741.pdf http://www.lume.ufrgs.br/bitstream/10183/165870/2/001044741.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/165870/3/001044741.pdf.jpg |
bitstream.checksum.fl_str_mv |
96d5028136cf9551de431ce6bb954cea 3046ada9d290b736928d565560206821 ca9ca38dfce6ede02a884a104bf964de |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
lume@ufrgs.br |
_version_ |
1817725008809558016 |