Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1)
Autor(a) principal: | |
---|---|
Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/224674 |
Resumo: | Background: The method of collection as well as the packaging conditions in which samples are submitted to laboratories play a critical role on the acquisition of reliable results on diagnostic tests. Alternative methods however have been proposed, as the adsorption of blood or serum in filter paper. In this work, it was evaluated the viability of using serum or whole blood samples from bovines collected in filter paper for serological testing against bovine herpesvirus type 1 (BoHV-1). Materials, Methods & Results: One hundred and seven whole blood and serum samples were collected by standard methods. Serum neutralization test was used as a golden standard method for evaluation of the serum samples. The same samples of both whole blood and sera were also adsorbed on filter paper strips for further comparisons. Optimal conditions for serum and blood elution from filter paper were determined. Adsorbed samples on filter paper disks were eluted in PBS and subsequently diluted further with PBS 5% skimmed milk. The eluates were tested for antibodies to BoHV-1 in an indirect ELISA (iELISA) and matched with the results obtained by serum neutralization of standard serum samples. Comparison between results obtained by serum neutralization of standard serum samples and the ones from the iELISA of serum eluted from paper disks resulted in sensitivity, specificity, positive and negative predictive values of 95, 94, 80, 99%, respectively, and a correlation coefficient (κ) of 0.83. Comparison between the results of serum neutralization of standard serum samples and the ones from the iELISA of blood eluted from paper disks resulted in sensitivity, specificity, positive and negative predictive values of 91, 97, 87, 98% , respectively, and a correlation coefficient (κ) of 0.86. Standard serum samples were also tested in the iELISA and the results compared with those of iELISA from samples eluted from filter paper. Comparison of the results from iELISA between serum samples and serum eluted from filter paper resulted in sensitivity, specificity, positive and negative predictive values of 91, 81, 69, 95%, respectively, and a correlation coefficient (κ) of 0.66. The comparison of iELISA results between standard serum and blood samples eluted from filter paper resulted, in sensitivity, specificity, positive and negative predictive values of 78, 92, 82, 89%, respectively, and a correlation coefficient (κ) of 0.70. Fifty samples collected on filter paper were retested eight months later in order to determine whether those would retain its viability; both sensitivity and specificity remained unaltered. Discussion: Sampling on filter paper has been successfully described for antibody detection in a number of diseases such as Aujeszky’s disease virus and Newcastle disease virus. In this work, it has been demonstrated that both blood and sera collected in filter paper can be used for submission of samples aiming detection of antibodies to BoHV-1 in an iELISA, without significant loss of sensitivity and specificity. Submission of samples on filter paper is a practical and economical alternative as no special conditions are required for storaging and transporting. This method also enables the collection of samples from distant places assuring its quality for serological tests |
id |
UFRGS-2_a92a238692506592b13163518a308fdb |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/224674 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Oliveira, Anna Paula deDavid, Cintia deEsteves, Paulo AugustoSpilki, Fernando RosadoSilva, Alessandra D'Avila daHolz, Carine LidianeSimonetti, Amauri BragaRoehe, Paulo Michel2021-07-28T04:41:07Z20111678-0345http://hdl.handle.net/10183/224674000819750Background: The method of collection as well as the packaging conditions in which samples are submitted to laboratories play a critical role on the acquisition of reliable results on diagnostic tests. Alternative methods however have been proposed, as the adsorption of blood or serum in filter paper. In this work, it was evaluated the viability of using serum or whole blood samples from bovines collected in filter paper for serological testing against bovine herpesvirus type 1 (BoHV-1). Materials, Methods & Results: One hundred and seven whole blood and serum samples were collected by standard methods. Serum neutralization test was used as a golden standard method for evaluation of the serum samples. The same samples of both whole blood and sera were also adsorbed on filter paper strips for further comparisons. Optimal conditions for serum and blood elution from filter paper were determined. Adsorbed samples on filter paper disks were eluted in PBS and subsequently diluted further with PBS 5% skimmed milk. The eluates were tested for antibodies to BoHV-1 in an indirect ELISA (iELISA) and matched with the results obtained by serum neutralization of standard serum samples. Comparison between results obtained by serum neutralization of standard serum samples and the ones from the iELISA of serum eluted from paper disks resulted in sensitivity, specificity, positive and negative predictive values of 95, 94, 80, 99%, respectively, and a correlation coefficient (κ) of 0.83. Comparison between the results of serum neutralization of standard serum samples and the ones from the iELISA of blood eluted from paper disks resulted in sensitivity, specificity, positive and negative predictive values of 91, 97, 87, 98% , respectively, and a correlation coefficient (κ) of 0.86. Standard serum samples were also tested in the iELISA and the results compared with those of iELISA from samples eluted from filter paper. Comparison of the results from iELISA between serum samples and serum eluted from filter paper resulted in sensitivity, specificity, positive and negative predictive values of 91, 81, 69, 95%, respectively, and a correlation coefficient (κ) of 0.66. The comparison of iELISA results between standard serum and blood samples eluted from filter paper resulted, in sensitivity, specificity, positive and negative predictive values of 78, 92, 82, 89%, respectively, and a correlation coefficient (κ) of 0.70. Fifty samples collected on filter paper were retested eight months later in order to determine whether those would retain its viability; both sensitivity and specificity remained unaltered. Discussion: Sampling on filter paper has been successfully described for antibody detection in a number of diseases such as Aujeszky’s disease virus and Newcastle disease virus. In this work, it has been demonstrated that both blood and sera collected in filter paper can be used for submission of samples aiming detection of antibodies to BoHV-1 in an iELISA, without significant loss of sensitivity and specificity. Submission of samples on filter paper is a practical and economical alternative as no special conditions are required for storaging and transporting. This method also enables the collection of samples from distant places assuring its quality for serological testsapplication/pdfengActa scientiae veterinariae. Porto Alegre. Vol. 39, no. 1 (2011), pub. 948, 6 f.SoroELISAHerpesvirus bovino 1FiltrosSerum neutralizationIndirect ELISABovine herpesvirusFilter paper adsorbed samplesBlood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1)info:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000819750.pdf.txt000819750.pdf.txtExtracted Texttext/plain23629http://www.lume.ufrgs.br/bitstream/10183/224674/2/000819750.pdf.txte4ea023152ea87e19fce248bd8a4c16cMD52ORIGINAL000819750.pdfTexto completo (inglês)application/pdf476466http://www.lume.ufrgs.br/bitstream/10183/224674/1/000819750.pdff9669f94792ff36bb919e40cd0914b9dMD5110183/2246742024-05-26 06:38:52.059674oai:www.lume.ufrgs.br:10183/224674Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-05-26T09:38:52Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1) |
title |
Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1) |
spellingShingle |
Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1) Oliveira, Anna Paula de Soro ELISA Herpesvirus bovino 1 Filtros Serum neutralization Indirect ELISA Bovine herpesvirus Filter paper adsorbed samples |
title_short |
Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1) |
title_full |
Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1) |
title_fullStr |
Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1) |
title_full_unstemmed |
Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1) |
title_sort |
Blood or serum collected on filter paper for detection of antibodies to bovine herpesvirus type 1 (BoHV-1) |
author |
Oliveira, Anna Paula de |
author_facet |
Oliveira, Anna Paula de David, Cintia de Esteves, Paulo Augusto Spilki, Fernando Rosado Silva, Alessandra D'Avila da Holz, Carine Lidiane Simonetti, Amauri Braga Roehe, Paulo Michel |
author_role |
author |
author2 |
David, Cintia de Esteves, Paulo Augusto Spilki, Fernando Rosado Silva, Alessandra D'Avila da Holz, Carine Lidiane Simonetti, Amauri Braga Roehe, Paulo Michel |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, Anna Paula de David, Cintia de Esteves, Paulo Augusto Spilki, Fernando Rosado Silva, Alessandra D'Avila da Holz, Carine Lidiane Simonetti, Amauri Braga Roehe, Paulo Michel |
dc.subject.por.fl_str_mv |
Soro ELISA Herpesvirus bovino 1 Filtros |
topic |
Soro ELISA Herpesvirus bovino 1 Filtros Serum neutralization Indirect ELISA Bovine herpesvirus Filter paper adsorbed samples |
dc.subject.eng.fl_str_mv |
Serum neutralization Indirect ELISA Bovine herpesvirus Filter paper adsorbed samples |
description |
Background: The method of collection as well as the packaging conditions in which samples are submitted to laboratories play a critical role on the acquisition of reliable results on diagnostic tests. Alternative methods however have been proposed, as the adsorption of blood or serum in filter paper. In this work, it was evaluated the viability of using serum or whole blood samples from bovines collected in filter paper for serological testing against bovine herpesvirus type 1 (BoHV-1). Materials, Methods & Results: One hundred and seven whole blood and serum samples were collected by standard methods. Serum neutralization test was used as a golden standard method for evaluation of the serum samples. The same samples of both whole blood and sera were also adsorbed on filter paper strips for further comparisons. Optimal conditions for serum and blood elution from filter paper were determined. Adsorbed samples on filter paper disks were eluted in PBS and subsequently diluted further with PBS 5% skimmed milk. The eluates were tested for antibodies to BoHV-1 in an indirect ELISA (iELISA) and matched with the results obtained by serum neutralization of standard serum samples. Comparison between results obtained by serum neutralization of standard serum samples and the ones from the iELISA of serum eluted from paper disks resulted in sensitivity, specificity, positive and negative predictive values of 95, 94, 80, 99%, respectively, and a correlation coefficient (κ) of 0.83. Comparison between the results of serum neutralization of standard serum samples and the ones from the iELISA of blood eluted from paper disks resulted in sensitivity, specificity, positive and negative predictive values of 91, 97, 87, 98% , respectively, and a correlation coefficient (κ) of 0.86. Standard serum samples were also tested in the iELISA and the results compared with those of iELISA from samples eluted from filter paper. Comparison of the results from iELISA between serum samples and serum eluted from filter paper resulted in sensitivity, specificity, positive and negative predictive values of 91, 81, 69, 95%, respectively, and a correlation coefficient (κ) of 0.66. The comparison of iELISA results between standard serum and blood samples eluted from filter paper resulted, in sensitivity, specificity, positive and negative predictive values of 78, 92, 82, 89%, respectively, and a correlation coefficient (κ) of 0.70. Fifty samples collected on filter paper were retested eight months later in order to determine whether those would retain its viability; both sensitivity and specificity remained unaltered. Discussion: Sampling on filter paper has been successfully described for antibody detection in a number of diseases such as Aujeszky’s disease virus and Newcastle disease virus. In this work, it has been demonstrated that both blood and sera collected in filter paper can be used for submission of samples aiming detection of antibodies to BoHV-1 in an iELISA, without significant loss of sensitivity and specificity. Submission of samples on filter paper is a practical and economical alternative as no special conditions are required for storaging and transporting. This method also enables the collection of samples from distant places assuring its quality for serological tests |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2021-07-28T04:41:07Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/224674 |
dc.identifier.issn.pt_BR.fl_str_mv |
1678-0345 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000819750 |
identifier_str_mv |
1678-0345 000819750 |
url |
http://hdl.handle.net/10183/224674 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Acta scientiae veterinariae. Porto Alegre. Vol. 39, no. 1 (2011), pub. 948, 6 f. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/224674/2/000819750.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/224674/1/000819750.pdf |
bitstream.checksum.fl_str_mv |
e4ea023152ea87e19fce248bd8a4c16c f9669f94792ff36bb919e40cd0914b9d |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1801225028666130432 |