Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/225941 |
Resumo: | N-acetylcysteine (NAC) inhibits nociceptive transmission. This effect has been associated partly with its antioxidant properties. However, the effect of NAC on the levels of lipid hydroperoxides (a pro-oxidant marker), content of ascorbic acid (a key antioxidant molecule of nervous tissue) and total antioxidant capacity (TAC) is unknown. Thus, our study assessed these parameters in the lumbosacral spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve, one of the most commonly employed animal models of neuropathic pain. Thirty-six male Wistar rats weighing 200–300 g were equally divided into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve). All rats received intraperitoneal injections of NAC (150 mg kg–1 day–1 ) or saline for 1, 3, or 7 days. Rats were killed 1, 3, and 7 days after surgery. NAC treatment prevented the CCI-induced increase in lipid hydroperoxide levels only at day 1, although the amount was higher than that found in naive rats. NAC treatment also prevented the CCI-induced increase in ascorbic acid content, which occurred at days 1, 3, and 7. No significant change was found in TAC with NAC treatment. The changes observed here may be related to the antinociceptive effect of NAC because modulation of oxidative-stress parameters seemed to help normalize the spinal cord oxidative status altered by pain. |
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Horst, AndréaSouza, Jéssica Araújo deSantos, Maria do Carmo QuevedoRiffel, Ana Paula KonzenKolberg, CarolinaPartata, Wania Aparecida2021-08-20T04:09:36Z20170100-879Xhttp://hdl.handle.net/10183/225941001060051N-acetylcysteine (NAC) inhibits nociceptive transmission. This effect has been associated partly with its antioxidant properties. However, the effect of NAC on the levels of lipid hydroperoxides (a pro-oxidant marker), content of ascorbic acid (a key antioxidant molecule of nervous tissue) and total antioxidant capacity (TAC) is unknown. Thus, our study assessed these parameters in the lumbosacral spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve, one of the most commonly employed animal models of neuropathic pain. Thirty-six male Wistar rats weighing 200–300 g were equally divided into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve). All rats received intraperitoneal injections of NAC (150 mg kg–1 day–1 ) or saline for 1, 3, or 7 days. Rats were killed 1, 3, and 7 days after surgery. NAC treatment prevented the CCI-induced increase in lipid hydroperoxide levels only at day 1, although the amount was higher than that found in naive rats. NAC treatment also prevented the CCI-induced increase in ascorbic acid content, which occurred at days 1, 3, and 7. No significant change was found in TAC with NAC treatment. The changes observed here may be related to the antinociceptive effect of NAC because modulation of oxidative-stress parameters seemed to help normalize the spinal cord oxidative status altered by pain.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 50, no. 12 (Oct. 2017), 7 p.NeuralgiaAcetilcisteínaPeróxidos lipídicosLipid hydroperoxidesTotal antioxidant capacityAscorbic acidChronic constriction injuryAntinociceptionEffects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic paininfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001060051.pdf.txt001060051.pdf.txtExtracted Texttext/plain31789http://www.lume.ufrgs.br/bitstream/10183/225941/2/001060051.pdf.txt549d82e2d4236984a85fb3fc12b92bc8MD52ORIGINAL001060051.pdfTexto completo (inglês)application/pdf982249http://www.lume.ufrgs.br/bitstream/10183/225941/1/001060051.pdff8a8fee2da0643b54f18039198171e50MD5110183/2259412021-11-20 05:51:36.50777oai:www.lume.ufrgs.br:10183/225941Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T07:51:36Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain |
title |
Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain |
spellingShingle |
Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain Horst, Andréa Neuralgia Acetilcisteína Peróxidos lipídicos Lipid hydroperoxides Total antioxidant capacity Ascorbic acid Chronic constriction injury Antinociception |
title_short |
Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain |
title_full |
Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain |
title_fullStr |
Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain |
title_full_unstemmed |
Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain |
title_sort |
Effects of N-acetylcysteine on spinal cord oxidative stress biomarkers in rats with neuropathic pain |
author |
Horst, Andréa |
author_facet |
Horst, Andréa Souza, Jéssica Araújo de Santos, Maria do Carmo Quevedo Riffel, Ana Paula Konzen Kolberg, Carolina Partata, Wania Aparecida |
author_role |
author |
author2 |
Souza, Jéssica Araújo de Santos, Maria do Carmo Quevedo Riffel, Ana Paula Konzen Kolberg, Carolina Partata, Wania Aparecida |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Horst, Andréa Souza, Jéssica Araújo de Santos, Maria do Carmo Quevedo Riffel, Ana Paula Konzen Kolberg, Carolina Partata, Wania Aparecida |
dc.subject.por.fl_str_mv |
Neuralgia Acetilcisteína Peróxidos lipídicos |
topic |
Neuralgia Acetilcisteína Peróxidos lipídicos Lipid hydroperoxides Total antioxidant capacity Ascorbic acid Chronic constriction injury Antinociception |
dc.subject.eng.fl_str_mv |
Lipid hydroperoxides Total antioxidant capacity Ascorbic acid Chronic constriction injury Antinociception |
description |
N-acetylcysteine (NAC) inhibits nociceptive transmission. This effect has been associated partly with its antioxidant properties. However, the effect of NAC on the levels of lipid hydroperoxides (a pro-oxidant marker), content of ascorbic acid (a key antioxidant molecule of nervous tissue) and total antioxidant capacity (TAC) is unknown. Thus, our study assessed these parameters in the lumbosacral spinal cord of rats with chronic constriction injury (CCI) of the sciatic nerve, one of the most commonly employed animal models of neuropathic pain. Thirty-six male Wistar rats weighing 200–300 g were equally divided into the following groups: Naive (rats did not undergo surgical manipulation); Sham (rats in which all surgical procedures involved in CCI were used except the ligature), and CCI (rats in which four ligatures were tied loosely around the right common sciatic nerve). All rats received intraperitoneal injections of NAC (150 mg kg–1 day–1 ) or saline for 1, 3, or 7 days. Rats were killed 1, 3, and 7 days after surgery. NAC treatment prevented the CCI-induced increase in lipid hydroperoxide levels only at day 1, although the amount was higher than that found in naive rats. NAC treatment also prevented the CCI-induced increase in ascorbic acid content, which occurred at days 1, 3, and 7. No significant change was found in TAC with NAC treatment. The changes observed here may be related to the antinociceptive effect of NAC because modulation of oxidative-stress parameters seemed to help normalize the spinal cord oxidative status altered by pain. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
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2021-08-20T04:09:36Z |
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http://hdl.handle.net/10183/225941 |
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0100-879X |
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001060051 |
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http://hdl.handle.net/10183/225941 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 50, no. 12 (Oct. 2017), 7 p. |
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