Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization

Detalhes bibliográficos
Autor(a) principal: Silva, Walter Orlando Beys da
Data de Publicação: 2003
Tipo de documento: Trabalho de conclusão de curso
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/275205
Resumo: Lipase from a Brazilian strain of Metarhizium anisopliae was investigated. The effect of different lipid sources added to basal medium was verified to improve enzyme production. Biomass was highest for sunflower oil (21.88 mg.mL⁻¹), however lipase specific activity was highest olive oil (108.38 U.mg⁻¹). In the presence of surfactants, the highest lipase activity always occurred when SDS, Tween 20 and Tween 80 were added after 50h M. anisopliae growth, being SDS (4.54± 0.46 U.mL⁻¹) and Tween 80 (4.15±1.13 U.mL⁻¹) the best surfactants. This is the first report investigating lipase production by an entomopathogenic fungus.
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spelling Silva, Walter Orlando Beys daVainstein, Marilene HenningSchrank, Augusto2024-05-01T06:50:54Z2003http://hdl.handle.net/10183/275205000396492Lipase from a Brazilian strain of Metarhizium anisopliae was investigated. The effect of different lipid sources added to basal medium was verified to improve enzyme production. Biomass was highest for sunflower oil (21.88 mg.mL⁻¹), however lipase specific activity was highest olive oil (108.38 U.mg⁻¹). In the presence of surfactants, the highest lipase activity always occurred when SDS, Tween 20 and Tween 80 were added after 50h M. anisopliae growth, being SDS (4.54± 0.46 U.mL⁻¹) and Tween 80 (4.15±1.13 U.mL⁻¹) the best surfactants. This is the first report investigating lipase production by an entomopathogenic fungus.application/pdfengLipaseMetarhizium anisopliae : Fungo entomopatogenicoEnzimasLipase : a novel Metarhizium anisopliae enzyme. Production and solubilizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal do Rio Grande do SulInstituto de BiociênciasPorto Alegre, BR-RS2003Ciências Biológicas: Ênfase Molecular, Celular e Funcional: Bachareladograduaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT000396492.pdf.txt000396492.pdf.txtExtracted Texttext/plain21690http://www.lume.ufrgs.br/bitstream/10183/275205/2/000396492.pdf.txt9fe5c1946e5705e1d71cdfc25a005449MD52ORIGINAL000396492.pdfTexto completoapplication/pdf2604295http://www.lume.ufrgs.br/bitstream/10183/275205/1/000396492.pdfff2d751ef91dc16c49b35a7487ad6283MD5110183/2752052024-10-12 06:50:02.005793oai:www.lume.ufrgs.br:10183/275205Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-10-12T09:50:02Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization
title Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization
spellingShingle Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization
Silva, Walter Orlando Beys da
Lipase
Metarhizium anisopliae : Fungo entomopatogenico
Enzimas
title_short Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization
title_full Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization
title_fullStr Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization
title_full_unstemmed Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization
title_sort Lipase : a novel Metarhizium anisopliae enzyme. Production and solubilization
author Silva, Walter Orlando Beys da
author_facet Silva, Walter Orlando Beys da
author_role author
dc.contributor.author.fl_str_mv Silva, Walter Orlando Beys da
dc.contributor.advisor1.fl_str_mv Vainstein, Marilene Henning
dc.contributor.advisor-co1.fl_str_mv Schrank, Augusto
contributor_str_mv Vainstein, Marilene Henning
Schrank, Augusto
dc.subject.por.fl_str_mv Lipase
Metarhizium anisopliae : Fungo entomopatogenico
Enzimas
topic Lipase
Metarhizium anisopliae : Fungo entomopatogenico
Enzimas
description Lipase from a Brazilian strain of Metarhizium anisopliae was investigated. The effect of different lipid sources added to basal medium was verified to improve enzyme production. Biomass was highest for sunflower oil (21.88 mg.mL⁻¹), however lipase specific activity was highest olive oil (108.38 U.mg⁻¹). In the presence of surfactants, the highest lipase activity always occurred when SDS, Tween 20 and Tween 80 were added after 50h M. anisopliae growth, being SDS (4.54± 0.46 U.mL⁻¹) and Tween 80 (4.15±1.13 U.mL⁻¹) the best surfactants. This is the first report investigating lipase production by an entomopathogenic fungus.
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2024-05-01T06:50:54Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/bachelorThesis
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