Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Diógenes
Data de Publicação: 2013
Outros Autores: Paris, Fernanda de, Paiva, Rodrigo Minuto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/94942
Resumo: Herpes simplex virus (H SV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fl uid samples has allowed the detection of viral DNA with high sensitivity and specifi city. Methods: Serial dilutions of quantifi ed commercial controls of each virus were subjected to an in-house nested-PCR technique. Results: The minimum detection limits for HSV and VZV were 5 and 10 copies/μL, respectively. Conclusions: The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies.
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spelling Rodrigues, DiógenesParis, Fernanda dePaiva, Rodrigo Minuto2014-05-06T02:04:56Z20130037-8682http://hdl.handle.net/10183/94942000906185Herpes simplex virus (H SV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fl uid samples has allowed the detection of viral DNA with high sensitivity and specifi city. Methods: Serial dilutions of quantifi ed commercial controls of each virus were subjected to an in-house nested-PCR technique. Results: The minimum detection limits for HSV and VZV were 5 and 10 copies/μL, respectively. Conclusions: The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies.application/pdfengRevista da Sociedade Brasileira de Medicina Tropical. Brasilia. Vol. 46, n. 5 (set./out. 2013), p. 625-628.SimplexvirusHerpesvirus Humano 3Reação em cadeia da polimerasePolymerase chain reactionenVaricella zoster virusHerpes simplex virusMinimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virusinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000906185.pdf000906185.pdfTexto completo (inglês)application/pdf815918http://www.lume.ufrgs.br/bitstream/10183/94942/1/000906185.pdf807d5283762964b6c61bb1f4c2cefebbMD51TEXT000906185.pdf.txt000906185.pdf.txtExtracted Texttext/plain17719http://www.lume.ufrgs.br/bitstream/10183/94942/2/000906185.pdf.txt198eb976a5b59eabc58cc6b2650657c2MD52THUMBNAIL000906185.pdf.jpg000906185.pdf.jpgGenerated Thumbnailimage/jpeg2372http://www.lume.ufrgs.br/bitstream/10183/94942/3/000906185.pdf.jpga1f153e53effc9761108049ffd48be7fMD5310183/949422021-05-07 05:10:14.473214oai:www.lume.ufrgs.br:10183/94942Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-05-07T08:10:14Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
spellingShingle Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
Rodrigues, Diógenes
Simplexvirus
Herpesvirus Humano 3
Reação em cadeia da polimerase
Polymerase chain reactionen
Varicella zoster virus
Herpes simplex virus
title_short Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title_full Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title_fullStr Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title_full_unstemmed Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
title_sort Minimum detection limit of an in-house nested-PCR assay for herpes simplex virus and varicella zoster virus
author Rodrigues, Diógenes
author_facet Rodrigues, Diógenes
Paris, Fernanda de
Paiva, Rodrigo Minuto
author_role author
author2 Paris, Fernanda de
Paiva, Rodrigo Minuto
author2_role author
author
dc.contributor.author.fl_str_mv Rodrigues, Diógenes
Paris, Fernanda de
Paiva, Rodrigo Minuto
dc.subject.por.fl_str_mv Simplexvirus
Herpesvirus Humano 3
Reação em cadeia da polimerase
topic Simplexvirus
Herpesvirus Humano 3
Reação em cadeia da polimerase
Polymerase chain reactionen
Varicella zoster virus
Herpes simplex virus
dc.subject.eng.fl_str_mv Polymerase chain reactionen
Varicella zoster virus
Herpes simplex virus
description Herpes simplex virus (H SV) and varicella zoster virus (VZV) are responsible for a variety of human diseases, including central nervous system diseases. The use of polymerase chain reaction (PCR) techniques on cerebrospinal fl uid samples has allowed the detection of viral DNA with high sensitivity and specifi city. Methods: Serial dilutions of quantifi ed commercial controls of each virus were subjected to an in-house nested-PCR technique. Results: The minimum detection limits for HSV and VZV were 5 and 10 copies/μL, respectively. Conclusions: The detection limit of nested-PCR for HSV and VZV in this study was similar to the limits found in previous studies.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-05-06T02:04:56Z
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/94942
dc.identifier.issn.pt_BR.fl_str_mv 0037-8682
dc.identifier.nrb.pt_BR.fl_str_mv 000906185
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dc.language.iso.fl_str_mv eng
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dc.relation.ispartof.pt_BR.fl_str_mv Revista da Sociedade Brasileira de Medicina Tropical. Brasilia. Vol. 46, n. 5 (set./out. 2013), p. 625-628.
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