The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/204026 |
Resumo: | The female lower genital tract harbors a complex microbial community essential for homeostasis and health. During pregnancy, the female body undergoes unique hormonal changes that contribute to weight gain as well as modulations in immune function that can afect microbiota composition. Several studies have described the vaginal microbiota of pregnant women from the USA, Europe and Mexico. Here we expand our knowledge about the vaginal microbial communities during the third trimester to healthy expectant Brazilian mothers. Vaginal samples were collected from patients delivering at the Hospital de Clínicas de Porto Alegre, Brazil. Microbial DNA was isolated from samples and the V4 region of the 16S rRNA gene was amplifed and sequenced using the PGM Ion Torrent. Brazilian pregnant women presented three distinct types of microbial community at the time of labor. Two microbial communities, Cluster 1 and Cluster 3, presented an overall dominance of Lactobacillus while Cluster 2 tended to present higher diversity and richness, with the presence of Pseudomonas, Prevotella and other vaginosis related bacteria. About half of the Brazilian mothers sampled here had dominance of L. iners. The proportion of mothers without dominance of any Lactobacillus was higher in Brazil (22%) compared to UK (2.4%) and USA, where this community type was not detected. The vaginal microbiota showed signifcant correlation with the composition of the babies’ gut microbiota (p-value=0.002 with a R2 of 15.8%). Mothers presenting diferent vaginal microbiota shared diferent microorganisms with their newborns, which would refect on initial colonizers of the developing newborns’ gut. |
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Dobbler, Priscila Caroline ThiagoMai, VolkerProcianoy, Renato SoibelmannSilveira, Rita de Cássia dos SantosCorso, Andréa LúciaRoesch, Luiz Fernando Wurdig2019-12-28T04:04:49Z20190959-3993http://hdl.handle.net/10183/204026001106589The female lower genital tract harbors a complex microbial community essential for homeostasis and health. During pregnancy, the female body undergoes unique hormonal changes that contribute to weight gain as well as modulations in immune function that can afect microbiota composition. Several studies have described the vaginal microbiota of pregnant women from the USA, Europe and Mexico. Here we expand our knowledge about the vaginal microbial communities during the third trimester to healthy expectant Brazilian mothers. Vaginal samples were collected from patients delivering at the Hospital de Clínicas de Porto Alegre, Brazil. Microbial DNA was isolated from samples and the V4 region of the 16S rRNA gene was amplifed and sequenced using the PGM Ion Torrent. Brazilian pregnant women presented three distinct types of microbial community at the time of labor. Two microbial communities, Cluster 1 and Cluster 3, presented an overall dominance of Lactobacillus while Cluster 2 tended to present higher diversity and richness, with the presence of Pseudomonas, Prevotella and other vaginosis related bacteria. About half of the Brazilian mothers sampled here had dominance of L. iners. The proportion of mothers without dominance of any Lactobacillus was higher in Brazil (22%) compared to UK (2.4%) and USA, where this community type was not detected. The vaginal microbiota showed signifcant correlation with the composition of the babies’ gut microbiota (p-value=0.002 with a R2 of 15.8%). Mothers presenting diferent vaginal microbiota shared diferent microorganisms with their newborns, which would refect on initial colonizers of the developing newborns’ gut.application/pdfengWorld Journal of Microbiology & Biotechnology. Vol. 35, no. 10 (2019), 159, 14 p.GravidezRecém-nascidoMicrobiotaVaginaMicrobioma gastrointestinalAnálise de sequência de RNARNA ribossômico 16STécnicas de amplificação de ácido nucleicoEstudo observacionalEstudos transversaisBrasil16S rRNAMicrobial diversityNext generation sequencingPregnancyVaginal microbiomeThe vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonizationEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001106589.pdf.txt001106589.pdf.txtExtracted Texttext/plain53453http://www.lume.ufrgs.br/bitstream/10183/204026/2/001106589.pdf.txt61f44a7ff89865c881b66bb79edd6598MD52ORIGINAL001106589.pdfTexto completo (inglês)application/pdf3716986http://www.lume.ufrgs.br/bitstream/10183/204026/1/001106589.pdf4fa4971730442ef42cdfc56ee8d13adcMD5110183/2040262019-12-29 05:04:07.113593oai:www.lume.ufrgs.br:10183/204026Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-12-29T07:04:07Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization |
title |
The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization |
spellingShingle |
The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization Dobbler, Priscila Caroline Thiago Gravidez Recém-nascido Microbiota Vagina Microbioma gastrointestinal Análise de sequência de RNA RNA ribossômico 16S Técnicas de amplificação de ácido nucleico Estudo observacional Estudos transversais Brasil 16S rRNA Microbial diversity Next generation sequencing Pregnancy Vaginal microbiome |
title_short |
The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization |
title_full |
The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization |
title_fullStr |
The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization |
title_full_unstemmed |
The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization |
title_sort |
The vaginal microbial communities of healthy expectant Brazilian mothers and its correlation with the newborn’s gut colonization |
author |
Dobbler, Priscila Caroline Thiago |
author_facet |
Dobbler, Priscila Caroline Thiago Mai, Volker Procianoy, Renato Soibelmann Silveira, Rita de Cássia dos Santos Corso, Andréa Lúcia Roesch, Luiz Fernando Wurdig |
author_role |
author |
author2 |
Mai, Volker Procianoy, Renato Soibelmann Silveira, Rita de Cássia dos Santos Corso, Andréa Lúcia Roesch, Luiz Fernando Wurdig |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Dobbler, Priscila Caroline Thiago Mai, Volker Procianoy, Renato Soibelmann Silveira, Rita de Cássia dos Santos Corso, Andréa Lúcia Roesch, Luiz Fernando Wurdig |
dc.subject.por.fl_str_mv |
Gravidez Recém-nascido Microbiota Vagina Microbioma gastrointestinal Análise de sequência de RNA RNA ribossômico 16S Técnicas de amplificação de ácido nucleico Estudo observacional Estudos transversais Brasil |
topic |
Gravidez Recém-nascido Microbiota Vagina Microbioma gastrointestinal Análise de sequência de RNA RNA ribossômico 16S Técnicas de amplificação de ácido nucleico Estudo observacional Estudos transversais Brasil 16S rRNA Microbial diversity Next generation sequencing Pregnancy Vaginal microbiome |
dc.subject.eng.fl_str_mv |
16S rRNA Microbial diversity Next generation sequencing Pregnancy Vaginal microbiome |
description |
The female lower genital tract harbors a complex microbial community essential for homeostasis and health. During pregnancy, the female body undergoes unique hormonal changes that contribute to weight gain as well as modulations in immune function that can afect microbiota composition. Several studies have described the vaginal microbiota of pregnant women from the USA, Europe and Mexico. Here we expand our knowledge about the vaginal microbial communities during the third trimester to healthy expectant Brazilian mothers. Vaginal samples were collected from patients delivering at the Hospital de Clínicas de Porto Alegre, Brazil. Microbial DNA was isolated from samples and the V4 region of the 16S rRNA gene was amplifed and sequenced using the PGM Ion Torrent. Brazilian pregnant women presented three distinct types of microbial community at the time of labor. Two microbial communities, Cluster 1 and Cluster 3, presented an overall dominance of Lactobacillus while Cluster 2 tended to present higher diversity and richness, with the presence of Pseudomonas, Prevotella and other vaginosis related bacteria. About half of the Brazilian mothers sampled here had dominance of L. iners. The proportion of mothers without dominance of any Lactobacillus was higher in Brazil (22%) compared to UK (2.4%) and USA, where this community type was not detected. The vaginal microbiota showed signifcant correlation with the composition of the babies’ gut microbiota (p-value=0.002 with a R2 of 15.8%). Mothers presenting diferent vaginal microbiota shared diferent microorganisms with their newborns, which would refect on initial colonizers of the developing newborns’ gut. |
publishDate |
2019 |
dc.date.accessioned.fl_str_mv |
2019-12-28T04:04:49Z |
dc.date.issued.fl_str_mv |
2019 |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/204026 |
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0959-3993 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001106589 |
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http://hdl.handle.net/10183/204026 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
World Journal of Microbiology & Biotechnology. Vol. 35, no. 10 (2019), 159, 14 p. |
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info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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