Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus

Detalhes bibliográficos
Autor(a) principal: Pereira, Mayra Ramos de Jesus
Data de Publicação: 2018
Outros Autores: Pinhatti, Valéria Rodrigues, Silveira, Maiele Dornelles da, Matzenbacher, Cristina Araujo, Freitas, Thales Renato Ochotorena de, Silva, Juliana da, Camassola, Melissa, Nardi, Nance Beyer
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/273744
Resumo: Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45, CD106, and MHC class II, as well as strong positive results for CD29. Low fluorescence levels were seen for CD49d, CD90.2 and CD117. Cultures were negative for CD49e, except for brain-derived cultures that were weakly positive. CD11b was negative in adipose-derived MSCs, but positive in brain and bone marrow-derived cultures. The scratch assay showed high migration potential for pancreas and adipose tissue-derived cells. This study represents the first report of isolation and characterization of cultures having characteristics of MSCs from Ctenomys minutus. The collection of biological information for biobanks represents an important contribution to the creation of strategies for prevention of loss of genetic diversity.
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spelling Pereira, Mayra Ramos de JesusPinhatti, Valéria RodriguesSilveira, Maiele Dornelles daMatzenbacher, Cristina AraujoFreitas, Thales Renato Ochotorena deSilva, Juliana daCamassola, MelissaNardi, Nance Beyer2024-03-19T05:03:40Z20181415-4757http://hdl.handle.net/10183/273744001171440Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45, CD106, and MHC class II, as well as strong positive results for CD29. Low fluorescence levels were seen for CD49d, CD90.2 and CD117. Cultures were negative for CD49e, except for brain-derived cultures that were weakly positive. CD11b was negative in adipose-derived MSCs, but positive in brain and bone marrow-derived cultures. The scratch assay showed high migration potential for pancreas and adipose tissue-derived cells. This study represents the first report of isolation and characterization of cultures having characteristics of MSCs from Ctenomys minutus. The collection of biological information for biobanks represents an important contribution to the creation of strategies for prevention of loss of genetic diversity.application/pdfengGenetics and molecular biology. Ribeirão Preto. Vol. 41, no. 4 (Oct/Dec.2018), p. 799-805Células-troncoTuco-tucosCtenomys minutusMesenchymal stem/stromal cellsIsolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutusinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001171440.pdf.txt001171440.pdf.txtExtracted Texttext/plain29088http://www.lume.ufrgs.br/bitstream/10183/273744/2/001171440.pdf.txt0fbba8d6010e533707ac0be53bc38050MD52ORIGINAL001171440.pdfTexto completo (inglês)application/pdf2501852http://www.lume.ufrgs.br/bitstream/10183/273744/1/001171440.pdf9ab46e8d8b088defe2bde27194db52c8MD5110183/2737442024-03-20 04:47:54.830257oai:www.lume.ufrgs.br:10183/273744Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-03-20T07:47:54Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
title Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
spellingShingle Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
Pereira, Mayra Ramos de Jesus
Células-tronco
Tuco-tucos
Ctenomys minutus
Mesenchymal stem/stromal cells
title_short Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
title_full Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
title_fullStr Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
title_full_unstemmed Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
title_sort Isolation and characterization of mesenchymal stem/stromal cells from Ctenomys minutus
author Pereira, Mayra Ramos de Jesus
author_facet Pereira, Mayra Ramos de Jesus
Pinhatti, Valéria Rodrigues
Silveira, Maiele Dornelles da
Matzenbacher, Cristina Araujo
Freitas, Thales Renato Ochotorena de
Silva, Juliana da
Camassola, Melissa
Nardi, Nance Beyer
author_role author
author2 Pinhatti, Valéria Rodrigues
Silveira, Maiele Dornelles da
Matzenbacher, Cristina Araujo
Freitas, Thales Renato Ochotorena de
Silva, Juliana da
Camassola, Melissa
Nardi, Nance Beyer
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Mayra Ramos de Jesus
Pinhatti, Valéria Rodrigues
Silveira, Maiele Dornelles da
Matzenbacher, Cristina Araujo
Freitas, Thales Renato Ochotorena de
Silva, Juliana da
Camassola, Melissa
Nardi, Nance Beyer
dc.subject.por.fl_str_mv Células-tronco
Tuco-tucos
Ctenomys minutus
topic Células-tronco
Tuco-tucos
Ctenomys minutus
Mesenchymal stem/stromal cells
dc.subject.eng.fl_str_mv Mesenchymal stem/stromal cells
description Mesenchymal stem/stromal cells (MSCs) are multipotent cells distributed in all tissues and characterized by adherence, morphology, immunophenotype and trilineage differentiation potential. The present study aimed to isolate and characterize adherent MSC-like populations from different tissues of Ctenomys minutus, a threatened wildlife rodent popularly known as tuco-tuco. Adherent cells were isolated from bone marrow, brain, liver, pancreas and adipose tissue of three adult animals collect in southern Brazil. Cultures showed typical morphology and proliferation potential. Adipose-derived MSCs showed trilineage potential. Cultures derived from adipose tissue, bone marrow and brain were immunophenotyped with negative results for CD31, CD44, CD45, CD106, and MHC class II, as well as strong positive results for CD29. Low fluorescence levels were seen for CD49d, CD90.2 and CD117. Cultures were negative for CD49e, except for brain-derived cultures that were weakly positive. CD11b was negative in adipose-derived MSCs, but positive in brain and bone marrow-derived cultures. The scratch assay showed high migration potential for pancreas and adipose tissue-derived cells. This study represents the first report of isolation and characterization of cultures having characteristics of MSCs from Ctenomys minutus. The collection of biological information for biobanks represents an important contribution to the creation of strategies for prevention of loss of genetic diversity.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2024-03-19T05:03:40Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/other
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/273744
dc.identifier.issn.pt_BR.fl_str_mv 1415-4757
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dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv Genetics and molecular biology. Ribeirão Preto. Vol. 41, no. 4 (Oct/Dec.2018), p. 799-805
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
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