Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/177616 |
Resumo: | The infection caused by Trichomonas vaginalis is the most common but overlooked nonviral sexually transmitted disease worldwide. Treatment relies on one class of drugs, the 5- nitroimidazoles, but resistance is widespread. New drugs are urgently needed. We reported the effect of crude and purified saponin fractions of Manilkara rufula against Trichomonas vaginalis. The compound responsible for antitrichomonal activity was isolated and identified as an uncommon bidesmosic saponin, Mi-saponin C. This saponin eliminated parasite viability without toxicity against the human vaginal epithelial line (HMVII). In addition, the isolated saponin fraction improved the metronidazole effect against a metronidazole-resistant isolate and dramatically reduced the cytoadherence of T. vaginalis to human cells. Investigation of the mechanism of death showed that the saponin fraction induced the parasite death due to profound membrane damage, inducing a disturbance of intracellular content without nuclear damage. To the best of our knowledge, this is the first report of antitrichomonal activity in the bidesmosic saponins of Manilkara rufula. |
id |
UFRGS-2_c3801301843b8d1b9ed2a580e118d264 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/177616 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Vieira, Patrícia de BrumSilva, Nícolas Luiz FeijóMenezes, Camila BrazSilva, Márcia Vanusa daSilva, Denise BrentanLopes, Norberto PeporineMacedo, Alexandre JoséBastida, JaumeTasca, Tiana2018-05-05T03:17:12Z20171932-6203http://hdl.handle.net/10183/177616001059068The infection caused by Trichomonas vaginalis is the most common but overlooked nonviral sexually transmitted disease worldwide. Treatment relies on one class of drugs, the 5- nitroimidazoles, but resistance is widespread. New drugs are urgently needed. We reported the effect of crude and purified saponin fractions of Manilkara rufula against Trichomonas vaginalis. The compound responsible for antitrichomonal activity was isolated and identified as an uncommon bidesmosic saponin, Mi-saponin C. This saponin eliminated parasite viability without toxicity against the human vaginal epithelial line (HMVII). In addition, the isolated saponin fraction improved the metronidazole effect against a metronidazole-resistant isolate and dramatically reduced the cytoadherence of T. vaginalis to human cells. Investigation of the mechanism of death showed that the saponin fraction induced the parasite death due to profound membrane damage, inducing a disturbance of intracellular content without nuclear damage. To the best of our knowledge, this is the first report of antitrichomonal activity in the bidesmosic saponins of Manilkara rufula.application/pdfengPLoS ONE. San Francisco. Vol. 12, no. 11 (Nov. 2017), e0188531, 20 p.Trichomonas vaginalisSaponinasTrichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufulaEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001059068.pdf001059068.pdfTexto completo (inglês)application/pdf8461197http://www.lume.ufrgs.br/bitstream/10183/177616/1/001059068.pdf5eeb23024c18c09b66c55afaa429cea1MD51TEXT001059068.pdf.txt001059068.pdf.txtExtracted Texttext/plain52860http://www.lume.ufrgs.br/bitstream/10183/177616/2/001059068.pdf.txt70bae60970ac359b8d543f0f21e12e4cMD5210183/1776162018-05-06 02:27:06.242952oai:www.lume.ufrgs.br:10183/177616Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2018-05-06T05:27:06Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula |
title |
Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula |
spellingShingle |
Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula Vieira, Patrícia de Brum Trichomonas vaginalis Saponinas |
title_short |
Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula |
title_full |
Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula |
title_fullStr |
Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula |
title_full_unstemmed |
Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula |
title_sort |
Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula |
author |
Vieira, Patrícia de Brum |
author_facet |
Vieira, Patrícia de Brum Silva, Nícolas Luiz Feijó Menezes, Camila Braz Silva, Márcia Vanusa da Silva, Denise Brentan Lopes, Norberto Peporine Macedo, Alexandre José Bastida, Jaume Tasca, Tiana |
author_role |
author |
author2 |
Silva, Nícolas Luiz Feijó Menezes, Camila Braz Silva, Márcia Vanusa da Silva, Denise Brentan Lopes, Norberto Peporine Macedo, Alexandre José Bastida, Jaume Tasca, Tiana |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vieira, Patrícia de Brum Silva, Nícolas Luiz Feijó Menezes, Camila Braz Silva, Márcia Vanusa da Silva, Denise Brentan Lopes, Norberto Peporine Macedo, Alexandre José Bastida, Jaume Tasca, Tiana |
dc.subject.por.fl_str_mv |
Trichomonas vaginalis Saponinas |
topic |
Trichomonas vaginalis Saponinas |
description |
The infection caused by Trichomonas vaginalis is the most common but overlooked nonviral sexually transmitted disease worldwide. Treatment relies on one class of drugs, the 5- nitroimidazoles, but resistance is widespread. New drugs are urgently needed. We reported the effect of crude and purified saponin fractions of Manilkara rufula against Trichomonas vaginalis. The compound responsible for antitrichomonal activity was isolated and identified as an uncommon bidesmosic saponin, Mi-saponin C. This saponin eliminated parasite viability without toxicity against the human vaginal epithelial line (HMVII). In addition, the isolated saponin fraction improved the metronidazole effect against a metronidazole-resistant isolate and dramatically reduced the cytoadherence of T. vaginalis to human cells. Investigation of the mechanism of death showed that the saponin fraction induced the parasite death due to profound membrane damage, inducing a disturbance of intracellular content without nuclear damage. To the best of our knowledge, this is the first report of antitrichomonal activity in the bidesmosic saponins of Manilkara rufula. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-05-05T03:17:12Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/177616 |
dc.identifier.issn.pt_BR.fl_str_mv |
1932-6203 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001059068 |
identifier_str_mv |
1932-6203 001059068 |
url |
http://hdl.handle.net/10183/177616 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PLoS ONE. San Francisco. Vol. 12, no. 11 (Nov. 2017), e0188531, 20 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/177616/1/001059068.pdf http://www.lume.ufrgs.br/bitstream/10183/177616/2/001059068.pdf.txt |
bitstream.checksum.fl_str_mv |
5eeb23024c18c09b66c55afaa429cea1 70bae60970ac359b8d543f0f21e12e4c |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
lume@ufrgs.br |
_version_ |
1817725023861866496 |