Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula

Detalhes bibliográficos
Autor(a) principal: Vieira, Patrícia de Brum
Data de Publicação: 2017
Outros Autores: Silva, Nícolas Luiz Feijó, Menezes, Camila Braz, Silva, Márcia Vanusa da, Silva, Denise Brentan, Lopes, Norberto Peporine, Macedo, Alexandre José, Bastida, Jaume, Tasca, Tiana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/177616
Resumo: The infection caused by Trichomonas vaginalis is the most common but overlooked nonviral sexually transmitted disease worldwide. Treatment relies on one class of drugs, the 5- nitroimidazoles, but resistance is widespread. New drugs are urgently needed. We reported the effect of crude and purified saponin fractions of Manilkara rufula against Trichomonas vaginalis. The compound responsible for antitrichomonal activity was isolated and identified as an uncommon bidesmosic saponin, Mi-saponin C. This saponin eliminated parasite viability without toxicity against the human vaginal epithelial line (HMVII). In addition, the isolated saponin fraction improved the metronidazole effect against a metronidazole-resistant isolate and dramatically reduced the cytoadherence of T. vaginalis to human cells. Investigation of the mechanism of death showed that the saponin fraction induced the parasite death due to profound membrane damage, inducing a disturbance of intracellular content without nuclear damage. To the best of our knowledge, this is the first report of antitrichomonal activity in the bidesmosic saponins of Manilkara rufula.
id UFRGS-2_c3801301843b8d1b9ed2a580e118d264
oai_identifier_str oai:www.lume.ufrgs.br:10183/177616
network_acronym_str UFRGS-2
network_name_str Repositório Institucional da UFRGS
repository_id_str
spelling Vieira, Patrícia de BrumSilva, Nícolas Luiz FeijóMenezes, Camila BrazSilva, Márcia Vanusa daSilva, Denise BrentanLopes, Norberto PeporineMacedo, Alexandre JoséBastida, JaumeTasca, Tiana2018-05-05T03:17:12Z20171932-6203http://hdl.handle.net/10183/177616001059068The infection caused by Trichomonas vaginalis is the most common but overlooked nonviral sexually transmitted disease worldwide. Treatment relies on one class of drugs, the 5- nitroimidazoles, but resistance is widespread. New drugs are urgently needed. We reported the effect of crude and purified saponin fractions of Manilkara rufula against Trichomonas vaginalis. The compound responsible for antitrichomonal activity was isolated and identified as an uncommon bidesmosic saponin, Mi-saponin C. This saponin eliminated parasite viability without toxicity against the human vaginal epithelial line (HMVII). In addition, the isolated saponin fraction improved the metronidazole effect against a metronidazole-resistant isolate and dramatically reduced the cytoadherence of T. vaginalis to human cells. Investigation of the mechanism of death showed that the saponin fraction induced the parasite death due to profound membrane damage, inducing a disturbance of intracellular content without nuclear damage. To the best of our knowledge, this is the first report of antitrichomonal activity in the bidesmosic saponins of Manilkara rufula.application/pdfengPLoS ONE. San Francisco. Vol. 12, no. 11 (Nov. 2017), e0188531, 20 p.Trichomonas vaginalisSaponinasTrichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufulaEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001059068.pdf001059068.pdfTexto completo (inglês)application/pdf8461197http://www.lume.ufrgs.br/bitstream/10183/177616/1/001059068.pdf5eeb23024c18c09b66c55afaa429cea1MD51TEXT001059068.pdf.txt001059068.pdf.txtExtracted Texttext/plain52860http://www.lume.ufrgs.br/bitstream/10183/177616/2/001059068.pdf.txt70bae60970ac359b8d543f0f21e12e4cMD5210183/1776162018-05-06 02:27:06.242952oai:www.lume.ufrgs.br:10183/177616Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2018-05-06T05:27:06Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
title Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
spellingShingle Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
Vieira, Patrícia de Brum
Trichomonas vaginalis
Saponinas
title_short Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
title_full Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
title_fullStr Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
title_full_unstemmed Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
title_sort Trichomonicidal and parasite membrane damaging activity of bidesmosic saponins from Manilkara rufula
author Vieira, Patrícia de Brum
author_facet Vieira, Patrícia de Brum
Silva, Nícolas Luiz Feijó
Menezes, Camila Braz
Silva, Márcia Vanusa da
Silva, Denise Brentan
Lopes, Norberto Peporine
Macedo, Alexandre José
Bastida, Jaume
Tasca, Tiana
author_role author
author2 Silva, Nícolas Luiz Feijó
Menezes, Camila Braz
Silva, Márcia Vanusa da
Silva, Denise Brentan
Lopes, Norberto Peporine
Macedo, Alexandre José
Bastida, Jaume
Tasca, Tiana
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vieira, Patrícia de Brum
Silva, Nícolas Luiz Feijó
Menezes, Camila Braz
Silva, Márcia Vanusa da
Silva, Denise Brentan
Lopes, Norberto Peporine
Macedo, Alexandre José
Bastida, Jaume
Tasca, Tiana
dc.subject.por.fl_str_mv Trichomonas vaginalis
Saponinas
topic Trichomonas vaginalis
Saponinas
description The infection caused by Trichomonas vaginalis is the most common but overlooked nonviral sexually transmitted disease worldwide. Treatment relies on one class of drugs, the 5- nitroimidazoles, but resistance is widespread. New drugs are urgently needed. We reported the effect of crude and purified saponin fractions of Manilkara rufula against Trichomonas vaginalis. The compound responsible for antitrichomonal activity was isolated and identified as an uncommon bidesmosic saponin, Mi-saponin C. This saponin eliminated parasite viability without toxicity against the human vaginal epithelial line (HMVII). In addition, the isolated saponin fraction improved the metronidazole effect against a metronidazole-resistant isolate and dramatically reduced the cytoadherence of T. vaginalis to human cells. Investigation of the mechanism of death showed that the saponin fraction induced the parasite death due to profound membrane damage, inducing a disturbance of intracellular content without nuclear damage. To the best of our knowledge, this is the first report of antitrichomonal activity in the bidesmosic saponins of Manilkara rufula.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-05-05T03:17:12Z
dc.type.driver.fl_str_mv Estrangeiro
info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10183/177616
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.nrb.pt_BR.fl_str_mv 001059068
identifier_str_mv 1932-6203
001059068
url http://hdl.handle.net/10183/177616
dc.language.iso.fl_str_mv eng
language eng
dc.relation.ispartof.pt_BR.fl_str_mv PLoS ONE. San Francisco. Vol. 12, no. 11 (Nov. 2017), e0188531, 20 p.
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRGS
instname:Universidade Federal do Rio Grande do Sul (UFRGS)
instacron:UFRGS
instname_str Universidade Federal do Rio Grande do Sul (UFRGS)
instacron_str UFRGS
institution UFRGS
reponame_str Repositório Institucional da UFRGS
collection Repositório Institucional da UFRGS
bitstream.url.fl_str_mv http://www.lume.ufrgs.br/bitstream/10183/177616/1/001059068.pdf
http://www.lume.ufrgs.br/bitstream/10183/177616/2/001059068.pdf.txt
bitstream.checksum.fl_str_mv 5eeb23024c18c09b66c55afaa429cea1
70bae60970ac359b8d543f0f21e12e4c
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)
repository.mail.fl_str_mv lume@ufrgs.br
_version_ 1817725023861866496