Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/150182 |
Resumo: | Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p<0.05) among the origins of the isolates (p<0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening. |
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Pilatti, Roberta MarmittFurian, Thales QuediLima, Daiane Alves deFinkler, FabrineBrito, Benito Guimarães deSalle, Carlos Tadeu PippiMoraes, Hamilton Luiz de Souza2016-12-17T02:16:59Z20161516-635Xhttp://hdl.handle.net/10183/150182000999604Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p<0.05) among the origins of the isolates (p<0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening.application/pdfengRevista brasileira de ciência avícola= Brazilian journal of poultry science. Vol. 18, n.2 (jun. 2016), p. 255-260Patologia aviariaPasteurella multocida : SuinosPatogenicidadeCasos clinicos : SuinosCasos clínicos : AvesFowl choleraInoculationPasteurella multocidaPathogenicity indexEstablishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swineinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000999604.pdf000999604.pdfTexto completo (inglês)application/pdf109470http://www.lume.ufrgs.br/bitstream/10183/150182/1/000999604.pdfa08fcca12b4424651dddff2d6218a3f0MD51TEXT000999604.pdf.txt000999604.pdf.txtExtracted Texttext/plain26566http://www.lume.ufrgs.br/bitstream/10183/150182/2/000999604.pdf.txta7d3a21d91bda51d9ee5c48584d91c09MD52THUMBNAIL000999604.pdf.jpg000999604.pdf.jpgGenerated Thumbnailimage/jpeg2082http://www.lume.ufrgs.br/bitstream/10183/150182/3/000999604.pdf.jpg388043462fa89dd1e2743f392709bb54MD5310183/1501822021-09-18 04:37:36.867368oai:www.lume.ufrgs.br:10183/150182Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-09-18T07:37:36Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine |
title |
Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine |
spellingShingle |
Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine Pilatti, Roberta Marmitt Patologia aviaria Pasteurella multocida : Suinos Patogenicidade Casos clinicos : Suinos Casos clínicos : Aves Fowl cholera Inoculation Pasteurella multocida Pathogenicity index |
title_short |
Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine |
title_full |
Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine |
title_fullStr |
Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine |
title_full_unstemmed |
Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine |
title_sort |
Establishment of a pathogenicity index for one-day-old broilers to Pasteurella multocida strains isolated from clinical cases in poultry and swine |
author |
Pilatti, Roberta Marmitt |
author_facet |
Pilatti, Roberta Marmitt Furian, Thales Quedi Lima, Daiane Alves de Finkler, Fabrine Brito, Benito Guimarães de Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza |
author_role |
author |
author2 |
Furian, Thales Quedi Lima, Daiane Alves de Finkler, Fabrine Brito, Benito Guimarães de Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pilatti, Roberta Marmitt Furian, Thales Quedi Lima, Daiane Alves de Finkler, Fabrine Brito, Benito Guimarães de Salle, Carlos Tadeu Pippi Moraes, Hamilton Luiz de Souza |
dc.subject.por.fl_str_mv |
Patologia aviaria Pasteurella multocida : Suinos Patogenicidade Casos clinicos : Suinos Casos clínicos : Aves |
topic |
Patologia aviaria Pasteurella multocida : Suinos Patogenicidade Casos clinicos : Suinos Casos clínicos : Aves Fowl cholera Inoculation Pasteurella multocida Pathogenicity index |
dc.subject.eng.fl_str_mv |
Fowl cholera Inoculation Pasteurella multocida Pathogenicity index |
description |
Although Pasteurella multocida is a member of the respiratory microbiota, under some circumstances, it is a primary agent of diseases , such as fowl cholera (FC), that cause significant economic losses. Experimental inoculations can be employed to evaluate the pathogenicity of strains, but the results are usually subjective and knowledge on the pathogenesis of this agent is still limited. The objective of this study was to establish a new methodology for classifying the pathogenicity of P. multocida by formulating a standard index. Strains isolated from FC cases and from swine with respiratory problems were selected. One hundred mL of a bacterial culture of each strain, containing 106 CFU, was inoculated in 10 one-day-old broilers. Mortality after inoculation, time of death (TD), and the presence of six macroscopic lesions were evaluated over a period of seven days post-inoculation (dpi). A Pathogenicity Index Per Bird (IPI), ranging 0 to 10, was calculated. Liver and heart fragments were collected to reisolate the bacteria. Blood was collected from the surviving birds, and an ELISA test was carried out to detect specific antibodies. The median of the pathogenicity indices, the number of lesions and the rate of bacteria reisolation were significantly different (p<0.05) among the origins of the isolates (p<0.05). The pathogenicity index developed in this study allows the classification of Pasteurella multocida pathogenicity and may be an alternative to the pathogenicity models currently used for screening. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-12-17T02:16:59Z |
dc.date.issued.fl_str_mv |
2016 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/150182 |
dc.identifier.issn.pt_BR.fl_str_mv |
1516-635X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000999604 |
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1516-635X 000999604 |
url |
http://hdl.handle.net/10183/150182 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Revista brasileira de ciência avícola= Brazilian journal of poultry science. Vol. 18, n.2 (jun. 2016), p. 255-260 |
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