Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Tipo de documento: | Trabalho de conclusão de curso |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/96145 |
Resumo: | Early life events lead to a large number of behavioral and biochemical alterations in adulthood. The aim of this study is to verify wheter the release of gonadal hormones during puberty affects parameters of oxidative stress observed in adulthood in cerebral cortex, striatum and hypothalamus of female rats subjected to neonatal handling. Rats were exposed or not to neonatal handling (10min/day, first 10 days of life). Between 21-28 post-natal days, females from each litter were divided into the following groups: ovariectomy, sham, and intact (no surgery). When adults, parameters of oxidative stress were analyzed. The groups subjected to surgery (ovx and sham), showed increased production of free radicals by the method of oxidation of dichlorodihydrofluorescein (DCFH) in cerebral cortex and striatum. Decreased catalase activity was observed in the cerebral cortex, in the same groups. No effects of neonatal manipulation were observed in these structures. We conclude that the period after weaning constitute a critical window for stressful interventions during development, leading to alterations in parameters of oxidative stress in adulthood, and these effects are not influenced by estradiol and neonatal handling. |
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Arcego, Danusa MarDalmaz, CarlaNoschang, Cristie Grazziotin2014-06-06T02:06:51Z2010http://hdl.handle.net/10183/96145000823409Early life events lead to a large number of behavioral and biochemical alterations in adulthood. The aim of this study is to verify wheter the release of gonadal hormones during puberty affects parameters of oxidative stress observed in adulthood in cerebral cortex, striatum and hypothalamus of female rats subjected to neonatal handling. Rats were exposed or not to neonatal handling (10min/day, first 10 days of life). Between 21-28 post-natal days, females from each litter were divided into the following groups: ovariectomy, sham, and intact (no surgery). When adults, parameters of oxidative stress were analyzed. The groups subjected to surgery (ovx and sham), showed increased production of free radicals by the method of oxidation of dichlorodihydrofluorescein (DCFH) in cerebral cortex and striatum. Decreased catalase activity was observed in the cerebral cortex, in the same groups. No effects of neonatal manipulation were observed in these structures. We conclude that the period after weaning constitute a critical window for stressful interventions during development, leading to alterations in parameters of oxidative stress in adulthood, and these effects are not influenced by estradiol and neonatal handling.application/pdfporEstresse oxidativo : RatosOvariectomiaManipulação neonatalNeonatal handlingEstradiolOxidative stressOvariectomySurgical stressPre-puberty periodIntervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultasEarly life interventions: evaluation of oxidative parameters in distinct cns structures in adult female rats info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bachelorThesisUniversidade Federal do Rio Grande do SulFaculdade de FarmáciaPorto Alegre, BR-RS2010Farmáciagraduaçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000823409.pdf000823409.pdfTexto completoapplication/pdf305187http://www.lume.ufrgs.br/bitstream/10183/96145/1/000823409.pdf0508ccc75590dc81224617d1dd82226fMD51TEXT000823409.pdf.txt000823409.pdf.txtExtracted Texttext/plain55854http://www.lume.ufrgs.br/bitstream/10183/96145/2/000823409.pdf.txtc84825be7e7c5bfd89c7cf901b16a048MD52THUMBNAIL000823409.pdf.jpg000823409.pdf.jpgGenerated Thumbnailimage/jpeg1121http://www.lume.ufrgs.br/bitstream/10183/96145/3/000823409.pdf.jpg6f83358cf30b07f8c2e263570864b1aaMD5310183/961452018-10-17 08:34:55.491oai:www.lume.ufrgs.br:10183/96145Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-17T11:34:55Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas |
dc.title.alternative.en.fl_str_mv |
Early life interventions: evaluation of oxidative parameters in distinct cns structures in adult female rats |
title |
Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas |
spellingShingle |
Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas Arcego, Danusa Mar Estresse oxidativo : Ratos Ovariectomia Manipulação neonatal Neonatal handling Estradiol Oxidative stress Ovariectomy Surgical stress Pre-puberty period |
title_short |
Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas |
title_full |
Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas |
title_fullStr |
Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas |
title_full_unstemmed |
Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas |
title_sort |
Intervenções precoces e avaliação de parâmetros oxidativos em diferentes estruturas do sistema nervoso de ratas adultas |
author |
Arcego, Danusa Mar |
author_facet |
Arcego, Danusa Mar |
author_role |
author |
dc.contributor.author.fl_str_mv |
Arcego, Danusa Mar |
dc.contributor.advisor1.fl_str_mv |
Dalmaz, Carla |
dc.contributor.advisor-co1.fl_str_mv |
Noschang, Cristie Grazziotin |
contributor_str_mv |
Dalmaz, Carla Noschang, Cristie Grazziotin |
dc.subject.por.fl_str_mv |
Estresse oxidativo : Ratos Ovariectomia Manipulação neonatal |
topic |
Estresse oxidativo : Ratos Ovariectomia Manipulação neonatal Neonatal handling Estradiol Oxidative stress Ovariectomy Surgical stress Pre-puberty period |
dc.subject.eng.fl_str_mv |
Neonatal handling Estradiol Oxidative stress Ovariectomy Surgical stress Pre-puberty period |
description |
Early life events lead to a large number of behavioral and biochemical alterations in adulthood. The aim of this study is to verify wheter the release of gonadal hormones during puberty affects parameters of oxidative stress observed in adulthood in cerebral cortex, striatum and hypothalamus of female rats subjected to neonatal handling. Rats were exposed or not to neonatal handling (10min/day, first 10 days of life). Between 21-28 post-natal days, females from each litter were divided into the following groups: ovariectomy, sham, and intact (no surgery). When adults, parameters of oxidative stress were analyzed. The groups subjected to surgery (ovx and sham), showed increased production of free radicals by the method of oxidation of dichlorodihydrofluorescein (DCFH) in cerebral cortex and striatum. Decreased catalase activity was observed in the cerebral cortex, in the same groups. No effects of neonatal manipulation were observed in these structures. We conclude that the period after weaning constitute a critical window for stressful interventions during development, leading to alterations in parameters of oxidative stress in adulthood, and these effects are not influenced by estradiol and neonatal handling. |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2014-06-06T02:06:51Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/bachelorThesis |
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bachelorThesis |
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publishedVersion |
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