Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/23380 |
Resumo: | The amylase electrophoretic patterns of 10 Brazilian brewing-barley varieties with different malting grades and diastatic power were analyzed during the 7-day germination which occurs during the malting process. Intra and inter-variety genetic variability was observed at both the structural and regulatory level. In the first few days after germination all varieties showed a few active loci, all of them with low activity. In subsequent days, new loci became active and those already detected since early germination showed increased activity. All varieties showed a continuous increase in amylase synthesis until the 3rd and/or 4th day after germination. Some varieties maintained high amylase activity until the last day of germination, while others showed a decrease in activity on the 5th or 6th day. No specific band increased or decreased its intensity independently of the others. A total of 14 loci were detected, out of which only one locus was polymorphic, indicating very low structural genetic variability, with only 2.8% polymorphic loci, an average of 1.04 alleles per loci, and an average expected heterozygosity of only 0.7%. The mean intra-variety Jaccard similarity coefficient complement (1 - SJ) was 0.009. The mean intra-variety difference based on regulatory differences was higher (1 - SJ = 0.17) than that obtained based on structural differences, suggesting differential gene activation. Inter-variety differentiation also showed low structural variability, with 1 - SJ = 0.026 and a Nei genetic distance (D) value of 0.0076, and a remarkable increase in divergence caused by differential gene activation (1 - SJ = 0.34). These results indicate that regulatory polymorphism is the principal agent responsible for amylase variability in the barley varieties analyzed. |
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Georg-Kraemer, Janaína EndresCavalli, Suzana Smith2010-06-05T04:17:23Z20031415-4757http://hdl.handle.net/10183/23380000476016The amylase electrophoretic patterns of 10 Brazilian brewing-barley varieties with different malting grades and diastatic power were analyzed during the 7-day germination which occurs during the malting process. Intra and inter-variety genetic variability was observed at both the structural and regulatory level. In the first few days after germination all varieties showed a few active loci, all of them with low activity. In subsequent days, new loci became active and those already detected since early germination showed increased activity. All varieties showed a continuous increase in amylase synthesis until the 3rd and/or 4th day after germination. Some varieties maintained high amylase activity until the last day of germination, while others showed a decrease in activity on the 5th or 6th day. No specific band increased or decreased its intensity independently of the others. A total of 14 loci were detected, out of which only one locus was polymorphic, indicating very low structural genetic variability, with only 2.8% polymorphic loci, an average of 1.04 alleles per loci, and an average expected heterozygosity of only 0.7%. The mean intra-variety Jaccard similarity coefficient complement (1 - SJ) was 0.009. The mean intra-variety difference based on regulatory differences was higher (1 - SJ = 0.17) than that obtained based on structural differences, suggesting differential gene activation. Inter-variety differentiation also showed low structural variability, with 1 - SJ = 0.026 and a Nei genetic distance (D) value of 0.0076, and a remarkable increase in divergence caused by differential gene activation (1 - SJ = 0.34). These results indicate that regulatory polymorphism is the principal agent responsible for amylase variability in the barley varieties analyzed.application/pdfengGenetics and molecular biology. Ribeirão Preto. Vol. 26, no. 1 (Mar. 2003), p. 69-75GenéticaAmilaseCevadaIsoenzimaBarleyAmylasesIsoenzymesStructural and regulatory genetic differences.Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivarsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000476016.pdf000476016.pdfTexto completo (inglês)application/pdf305511http://www.lume.ufrgs.br/bitstream/10183/23380/1/000476016.pdfafabeccc923c501e8409a8c8b0554a52MD51TEXT000476016.pdf.txt000476016.pdf.txtExtracted Texttext/plain26648http://www.lume.ufrgs.br/bitstream/10183/23380/2/000476016.pdf.txt237b2e3a571cb49b22c9118da76d352eMD52THUMBNAIL000476016.pdf.jpg000476016.pdf.jpgGenerated Thumbnailimage/jpeg1859http://www.lume.ufrgs.br/bitstream/10183/23380/3/000476016.pdf.jpg5c3358550352e4760c343b8d1b710204MD5310183/233802018-10-09 07:59:28.522oai:www.lume.ufrgs.br:10183/23380Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-09T10:59:28Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
spellingShingle |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars Georg-Kraemer, Janaína Endres Genética Amilase Cevada Isoenzima Barley Amylases Isoenzymes Structural and regulatory genetic differences. |
title_short |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title_full |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title_fullStr |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title_full_unstemmed |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
title_sort |
Structural and regulatory differences in amylase isoenzymes in germinating Brazilian barley cultivars |
author |
Georg-Kraemer, Janaína Endres |
author_facet |
Georg-Kraemer, Janaína Endres Cavalli, Suzana Smith |
author_role |
author |
author2 |
Cavalli, Suzana Smith |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Georg-Kraemer, Janaína Endres Cavalli, Suzana Smith |
dc.subject.por.fl_str_mv |
Genética Amilase Cevada Isoenzima |
topic |
Genética Amilase Cevada Isoenzima Barley Amylases Isoenzymes Structural and regulatory genetic differences. |
dc.subject.eng.fl_str_mv |
Barley Amylases Isoenzymes Structural and regulatory genetic differences. |
description |
The amylase electrophoretic patterns of 10 Brazilian brewing-barley varieties with different malting grades and diastatic power were analyzed during the 7-day germination which occurs during the malting process. Intra and inter-variety genetic variability was observed at both the structural and regulatory level. In the first few days after germination all varieties showed a few active loci, all of them with low activity. In subsequent days, new loci became active and those already detected since early germination showed increased activity. All varieties showed a continuous increase in amylase synthesis until the 3rd and/or 4th day after germination. Some varieties maintained high amylase activity until the last day of germination, while others showed a decrease in activity on the 5th or 6th day. No specific band increased or decreased its intensity independently of the others. A total of 14 loci were detected, out of which only one locus was polymorphic, indicating very low structural genetic variability, with only 2.8% polymorphic loci, an average of 1.04 alleles per loci, and an average expected heterozygosity of only 0.7%. The mean intra-variety Jaccard similarity coefficient complement (1 - SJ) was 0.009. The mean intra-variety difference based on regulatory differences was higher (1 - SJ = 0.17) than that obtained based on structural differences, suggesting differential gene activation. Inter-variety differentiation also showed low structural variability, with 1 - SJ = 0.026 and a Nei genetic distance (D) value of 0.0076, and a remarkable increase in divergence caused by differential gene activation (1 - SJ = 0.34). These results indicate that regulatory polymorphism is the principal agent responsible for amylase variability in the barley varieties analyzed. |
publishDate |
2003 |
dc.date.issued.fl_str_mv |
2003 |
dc.date.accessioned.fl_str_mv |
2010-06-05T04:17:23Z |
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000476016 |
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http://hdl.handle.net/10183/23380 |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto. Vol. 26, no. 1 (Mar. 2003), p. 69-75 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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