Effect of soybean ureases on seed germination and plant development
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/166137 |
Resumo: | Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5- co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes, as well as a urease-null mutant (eu3-a) that activates neither the ubiquitous nor embryo-specific ureases. The co-suppressed plants presented a developmental delay during the first month after germination; shoots and roots were significantly smaller and lighter. Slower development was observed for the double eu1-a/eu4-a mutant and the eu3-a single mutant. The N content in transgenic plants was significantly lower than in non-transgenic plants. Among the mutants, eu3-a presented the lowest and eu1-a the highest N content. Altogether, these results indicate that increased ureolytic activity plays an important role in plant development. |
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Rechenmacher, CilianaStrohm, Beatriz WiebkeBusatto, Luisa Abruzzi de OliveiraPolacco, Joseph CarmineCarlini, Celia Regina Ribeiro da SilvaBodanese-Zanettini, Maria Helena2017-09-05T02:32:35Z20171415-4757http://hdl.handle.net/10183/166137001026729Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5- co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes, as well as a urease-null mutant (eu3-a) that activates neither the ubiquitous nor embryo-specific ureases. The co-suppressed plants presented a developmental delay during the first month after germination; shoots and roots were significantly smaller and lighter. Slower development was observed for the double eu1-a/eu4-a mutant and the eu3-a single mutant. The N content in transgenic plants was significantly lower than in non-transgenic plants. Among the mutants, eu3-a presented the lowest and eu1-a the highest N content. Altogether, these results indicate that increased ureolytic activity plays an important role in plant development.application/pdfengGenetics and molecular biology. Ribeirão Preto, SP. Vol. 40, no. 1, suppl. 1 (2017), p. 209-216Desenvolvimento vegetalPlantas transgênicasUreaseSojaPlant developmentFunctional studyTransgenic plantsMutantsUreaseEffect of soybean ureases on seed germination and plant developmentinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL001026729.pdf001026729.pdfTexto completo (inglês)application/pdf1342450http://www.lume.ufrgs.br/bitstream/10183/166137/1/001026729.pdfc2afe969092a428b790167b13ca77a9cMD51TEXT001026729.pdf.txt001026729.pdf.txtExtracted Texttext/plain38063http://www.lume.ufrgs.br/bitstream/10183/166137/2/001026729.pdf.txt5984fb621efbab2f150bc474a2ef2fabMD52THUMBNAIL001026729.pdf.jpg001026729.pdf.jpgGenerated Thumbnailimage/jpeg1780http://www.lume.ufrgs.br/bitstream/10183/166137/3/001026729.pdf.jpgc21b7c0b776856f3c847cb005c7b12adMD5310183/1661372022-11-06 05:37:17.517851oai:www.lume.ufrgs.br:10183/166137Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2022-11-06T07:37:17Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Effect of soybean ureases on seed germination and plant development |
title |
Effect of soybean ureases on seed germination and plant development |
spellingShingle |
Effect of soybean ureases on seed germination and plant development Rechenmacher, Ciliana Desenvolvimento vegetal Plantas transgênicas Urease Soja Plant development Functional study Transgenic plants Mutants Urease |
title_short |
Effect of soybean ureases on seed germination and plant development |
title_full |
Effect of soybean ureases on seed germination and plant development |
title_fullStr |
Effect of soybean ureases on seed germination and plant development |
title_full_unstemmed |
Effect of soybean ureases on seed germination and plant development |
title_sort |
Effect of soybean ureases on seed germination and plant development |
author |
Rechenmacher, Ciliana |
author_facet |
Rechenmacher, Ciliana Strohm, Beatriz Wiebke Busatto, Luisa Abruzzi de Oliveira Polacco, Joseph Carmine Carlini, Celia Regina Ribeiro da Silva Bodanese-Zanettini, Maria Helena |
author_role |
author |
author2 |
Strohm, Beatriz Wiebke Busatto, Luisa Abruzzi de Oliveira Polacco, Joseph Carmine Carlini, Celia Regina Ribeiro da Silva Bodanese-Zanettini, Maria Helena |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rechenmacher, Ciliana Strohm, Beatriz Wiebke Busatto, Luisa Abruzzi de Oliveira Polacco, Joseph Carmine Carlini, Celia Regina Ribeiro da Silva Bodanese-Zanettini, Maria Helena |
dc.subject.por.fl_str_mv |
Desenvolvimento vegetal Plantas transgênicas Urease Soja |
topic |
Desenvolvimento vegetal Plantas transgênicas Urease Soja Plant development Functional study Transgenic plants Mutants Urease |
dc.subject.eng.fl_str_mv |
Plant development Functional study Transgenic plants Mutants Urease |
description |
Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5- co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes, as well as a urease-null mutant (eu3-a) that activates neither the ubiquitous nor embryo-specific ureases. The co-suppressed plants presented a developmental delay during the first month after germination; shoots and roots were significantly smaller and lighter. Slower development was observed for the double eu1-a/eu4-a mutant and the eu3-a single mutant. The N content in transgenic plants was significantly lower than in non-transgenic plants. Among the mutants, eu3-a presented the lowest and eu1-a the highest N content. Altogether, these results indicate that increased ureolytic activity plays an important role in plant development. |
publishDate |
2017 |
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2017-09-05T02:32:35Z |
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2017 |
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1415-4757 |
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001026729 |
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http://hdl.handle.net/10183/166137 |
dc.language.iso.fl_str_mv |
eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Genetics and molecular biology. Ribeirão Preto, SP. Vol. 40, no. 1, suppl. 1 (2017), p. 209-216 |
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