Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/100141 |
Resumo: | The objective of the present study was to assess the growth and the recovery of Salmonella (S.) Enteritidis SE86 in different diluents, culture media and using different plating methods after the exposure to 200 mg/kg sodium dichloroisocyanurate (NaDCC). Before and after NaDCC exposure, SE86 was cultured at 30 °C and 7 °C in the following diluents: Peptone water (P), Saline solution (SaS), Peptone water+Saline solution (P+SaS), Peptone water+Tween 80+Lecithin+Sodium thiosulfate (P+N) and Saline solution+Tween 80+Lecithin+Sodium thiosulfate (SaS+N). The SaS diluent was chosen because it was able to maintain cells viable without growth and was further used for plating SE86 on non selective medium (Tryptic Soy Agar-TSA) and on selective media (Mannitol Lysine Crystal Violet Brilliant Green Agar-MLCB; Brilliant Green Agar-BGA; Salmonella Shigella Agar-SS and Xylose Lysine Dextrose–XLD). The Thin Agar Layer method (TAL) i.e., selective media overlayed with non selective TSA was also evaluated. Results indicated that SE86 not exposed to NaDCC was able to grow in P, P+N, SaS+N and P+SaS, but not in SaS, that was able to maintain cells viable. SE86 exposed to NaDCC demonstrated similar counts after dilution in SaS and the plating on non selective TSA, selective media MLCB, BGA, SS and XLD and on TAL media. SE86, S. Typhimurium and S. Bredeney, exposed or not exposed to NaDCC, showed no significant differences in counts on TSA, XLD and XLD overlayed with TSA, suggesting that all those media may be used to quantify NaDCC-exposed Salmonella by plating method. |
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Ferreira, Fernanda StodutoHorvath, Mariana BandeiraTondo, Eduardo Cesar2014-08-12T02:10:47Z20131517-8382http://hdl.handle.net/10183/100141000921482The objective of the present study was to assess the growth and the recovery of Salmonella (S.) Enteritidis SE86 in different diluents, culture media and using different plating methods after the exposure to 200 mg/kg sodium dichloroisocyanurate (NaDCC). Before and after NaDCC exposure, SE86 was cultured at 30 °C and 7 °C in the following diluents: Peptone water (P), Saline solution (SaS), Peptone water+Saline solution (P+SaS), Peptone water+Tween 80+Lecithin+Sodium thiosulfate (P+N) and Saline solution+Tween 80+Lecithin+Sodium thiosulfate (SaS+N). The SaS diluent was chosen because it was able to maintain cells viable without growth and was further used for plating SE86 on non selective medium (Tryptic Soy Agar-TSA) and on selective media (Mannitol Lysine Crystal Violet Brilliant Green Agar-MLCB; Brilliant Green Agar-BGA; Salmonella Shigella Agar-SS and Xylose Lysine Dextrose–XLD). The Thin Agar Layer method (TAL) i.e., selective media overlayed with non selective TSA was also evaluated. Results indicated that SE86 not exposed to NaDCC was able to grow in P, P+N, SaS+N and P+SaS, but not in SaS, that was able to maintain cells viable. SE86 exposed to NaDCC demonstrated similar counts after dilution in SaS and the plating on non selective TSA, selective media MLCB, BGA, SS and XLD and on TAL media. SE86, S. Typhimurium and S. Bredeney, exposed or not exposed to NaDCC, showed no significant differences in counts on TSA, XLD and XLD overlayed with TSA, suggesting that all those media may be used to quantify NaDCC-exposed Salmonella by plating method.application/pdfengBrazilian journal of microbiology. São Paulo. Vol. 44, no. 3 (July/Sept. 2013), p. 785-790Salmonella enteritidisSalmonella entericaSalmonella enteritidis SE86DiluentsPlating methodsStressed cellsSodium dichloroisocyanurateAssessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposureinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000921482.pdf000921482.pdfTexto completo (inglês)application/pdf772588http://www.lume.ufrgs.br/bitstream/10183/100141/1/000921482.pdf4cc613a994f12438114069167b4afd71MD51TEXT000921482.pdf.txt000921482.pdf.txtExtracted Texttext/plain26650http://www.lume.ufrgs.br/bitstream/10183/100141/2/000921482.pdf.txt0ac713a1feabc72c1a66c91e76d7a034MD52THUMBNAIL000921482.pdf.jpg000921482.pdf.jpgGenerated Thumbnailimage/jpeg1705http://www.lume.ufrgs.br/bitstream/10183/100141/3/000921482.pdf.jpg73ba9f4f8b26dcb6cb4a0ef4fe490e0cMD5310183/1001412024-01-31 06:00:51.207633oai:www.lume.ufrgs.br:10183/100141Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2024-01-31T08:00:51Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure |
title |
Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure |
spellingShingle |
Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure Ferreira, Fernanda Stoduto Salmonella enteritidis Salmonella enterica Salmonella enteritidis SE86 Diluents Plating methods Stressed cells Sodium dichloroisocyanurate |
title_short |
Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure |
title_full |
Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure |
title_fullStr |
Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure |
title_full_unstemmed |
Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure |
title_sort |
Assessing the growth and recovery of Salmonella Enteritidis SE86 after sodium dichloroisocyanurate exposure |
author |
Ferreira, Fernanda Stoduto |
author_facet |
Ferreira, Fernanda Stoduto Horvath, Mariana Bandeira Tondo, Eduardo Cesar |
author_role |
author |
author2 |
Horvath, Mariana Bandeira Tondo, Eduardo Cesar |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Ferreira, Fernanda Stoduto Horvath, Mariana Bandeira Tondo, Eduardo Cesar |
dc.subject.por.fl_str_mv |
Salmonella enteritidis Salmonella enterica |
topic |
Salmonella enteritidis Salmonella enterica Salmonella enteritidis SE86 Diluents Plating methods Stressed cells Sodium dichloroisocyanurate |
dc.subject.eng.fl_str_mv |
Salmonella enteritidis SE86 Diluents Plating methods Stressed cells Sodium dichloroisocyanurate |
description |
The objective of the present study was to assess the growth and the recovery of Salmonella (S.) Enteritidis SE86 in different diluents, culture media and using different plating methods after the exposure to 200 mg/kg sodium dichloroisocyanurate (NaDCC). Before and after NaDCC exposure, SE86 was cultured at 30 °C and 7 °C in the following diluents: Peptone water (P), Saline solution (SaS), Peptone water+Saline solution (P+SaS), Peptone water+Tween 80+Lecithin+Sodium thiosulfate (P+N) and Saline solution+Tween 80+Lecithin+Sodium thiosulfate (SaS+N). The SaS diluent was chosen because it was able to maintain cells viable without growth and was further used for plating SE86 on non selective medium (Tryptic Soy Agar-TSA) and on selective media (Mannitol Lysine Crystal Violet Brilliant Green Agar-MLCB; Brilliant Green Agar-BGA; Salmonella Shigella Agar-SS and Xylose Lysine Dextrose–XLD). The Thin Agar Layer method (TAL) i.e., selective media overlayed with non selective TSA was also evaluated. Results indicated that SE86 not exposed to NaDCC was able to grow in P, P+N, SaS+N and P+SaS, but not in SaS, that was able to maintain cells viable. SE86 exposed to NaDCC demonstrated similar counts after dilution in SaS and the plating on non selective TSA, selective media MLCB, BGA, SS and XLD and on TAL media. SE86, S. Typhimurium and S. Bredeney, exposed or not exposed to NaDCC, showed no significant differences in counts on TSA, XLD and XLD overlayed with TSA, suggesting that all those media may be used to quantify NaDCC-exposed Salmonella by plating method. |
publishDate |
2013 |
dc.date.issued.fl_str_mv |
2013 |
dc.date.accessioned.fl_str_mv |
2014-08-12T02:10:47Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/100141 |
dc.identifier.issn.pt_BR.fl_str_mv |
1517-8382 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000921482 |
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url |
http://hdl.handle.net/10183/100141 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo. Vol. 44, no. 3 (July/Sept. 2013), p. 785-790 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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