Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats

Detalhes bibliográficos
Autor(a) principal: Hermel, Erica do Espirito Santo
Data de Publicação: 2001
Outros Autores: Severino, Gabriela Sentena, Cecconello, Ana Lúcia, Pereira, Francine Martins, Sanvitto, Gilberto Luiz, Lucion, Aldo Bolten
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/21129
Resumo: Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotinperoxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were counted bilaterally in the arcuate, paraventricular and periventricular nuclei of the hypothalamus in 30-μm sections at 120-μm intervals. Neonatal handling did not change the number of TH-IR neurons in the arcuate (1021 ± 206, N = 6; 1020 ± 150, N = 6; nonhandled and handled, respectively), paraventricular (584 ± 85, N = 8; 682 ± 62, N = 9) or periventricular (743 ± 118, N = 7; 990 ± 158, N = 7) nuclei of the hypothalamus. The absence of an effect on the number of dopaminergic cells in the hypothalamus indicates that the reduction in the amount of neurons induced by neonatal handling, as shown by other studies, is not a general phenomenon in the brain.
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spelling Hermel, Erica do Espirito SantoSeverino, Gabriela SentenaCecconello, Ana LúciaPereira, Francine MartinsSanvitto, Gilberto LuizLucion, Aldo Bolten2010-04-24T04:15:26Z20010100-879Xhttp://hdl.handle.net/10183/21129000324845Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotinperoxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were counted bilaterally in the arcuate, paraventricular and periventricular nuclei of the hypothalamus in 30-μm sections at 120-μm intervals. Neonatal handling did not change the number of TH-IR neurons in the arcuate (1021 ± 206, N = 6; 1020 ± 150, N = 6; nonhandled and handled, respectively), paraventricular (584 ± 85, N = 8; 682 ± 62, N = 9) or periventricular (743 ± 118, N = 7; 990 ± 158, N = 7) nuclei of the hypothalamus. The absence of an effect on the number of dopaminergic cells in the hypothalamus indicates that the reduction in the amount of neurons induced by neonatal handling, as shown by other studies, is not a general phenomenon in the brain.application/pdfengBrazilian journal of medical and biological research =Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 9 (Sept. 2001), p. 1191-1195FisiologiaStressNeonatal stimulationDopamineImmunohistochemistryHypothalamusTyrosine hydroxylaseNeonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000324845.pdf000324845.pdfTexto completo (inglês)application/pdf25451http://www.lume.ufrgs.br/bitstream/10183/21129/1/000324845.pdf251450c3f02e0b3a22a96f63804a5668MD51TEXT000324845.pdf.txt000324845.pdf.txtExtracted Texttext/plain16807http://www.lume.ufrgs.br/bitstream/10183/21129/2/000324845.pdf.txt9fdd6d95124ada67e661e0c28960acd0MD52THUMBNAIL000324845.pdf.jpg000324845.pdf.jpgGenerated Thumbnailimage/jpeg1732http://www.lume.ufrgs.br/bitstream/10183/21129/3/000324845.pdf.jpg3be30f2731b9574cb795c10c221d646aMD5310183/211292021-11-20 05:41:35.404412oai:www.lume.ufrgs.br:10183/21129Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T07:41:35Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
title Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
spellingShingle Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
Hermel, Erica do Espirito Santo
Fisiologia
Stress
Neonatal stimulation
Dopamine
Immunohistochemistry
Hypothalamus
Tyrosine hydroxylase
title_short Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
title_full Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
title_fullStr Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
title_full_unstemmed Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
title_sort Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
author Hermel, Erica do Espirito Santo
author_facet Hermel, Erica do Espirito Santo
Severino, Gabriela Sentena
Cecconello, Ana Lúcia
Pereira, Francine Martins
Sanvitto, Gilberto Luiz
Lucion, Aldo Bolten
author_role author
author2 Severino, Gabriela Sentena
Cecconello, Ana Lúcia
Pereira, Francine Martins
Sanvitto, Gilberto Luiz
Lucion, Aldo Bolten
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Hermel, Erica do Espirito Santo
Severino, Gabriela Sentena
Cecconello, Ana Lúcia
Pereira, Francine Martins
Sanvitto, Gilberto Luiz
Lucion, Aldo Bolten
dc.subject.por.fl_str_mv Fisiologia
topic Fisiologia
Stress
Neonatal stimulation
Dopamine
Immunohistochemistry
Hypothalamus
Tyrosine hydroxylase
dc.subject.eng.fl_str_mv Stress
Neonatal stimulation
Dopamine
Immunohistochemistry
Hypothalamus
Tyrosine hydroxylase
description Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotinperoxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were counted bilaterally in the arcuate, paraventricular and periventricular nuclei of the hypothalamus in 30-μm sections at 120-μm intervals. Neonatal handling did not change the number of TH-IR neurons in the arcuate (1021 ± 206, N = 6; 1020 ± 150, N = 6; nonhandled and handled, respectively), paraventricular (584 ± 85, N = 8; 682 ± 62, N = 9) or periventricular (743 ± 118, N = 7; 990 ± 158, N = 7) nuclei of the hypothalamus. The absence of an effect on the number of dopaminergic cells in the hypothalamus indicates that the reduction in the amount of neurons induced by neonatal handling, as shown by other studies, is not a general phenomenon in the brain.
publishDate 2001
dc.date.issued.fl_str_mv 2001
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dc.relation.ispartof.pt_BR.fl_str_mv Brazilian journal of medical and biological research =Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 9 (Sept. 2001), p. 1191-1195
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