Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21129 |
Resumo: | Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotinperoxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were counted bilaterally in the arcuate, paraventricular and periventricular nuclei of the hypothalamus in 30-μm sections at 120-μm intervals. Neonatal handling did not change the number of TH-IR neurons in the arcuate (1021 ± 206, N = 6; 1020 ± 150, N = 6; nonhandled and handled, respectively), paraventricular (584 ± 85, N = 8; 682 ± 62, N = 9) or periventricular (743 ± 118, N = 7; 990 ± 158, N = 7) nuclei of the hypothalamus. The absence of an effect on the number of dopaminergic cells in the hypothalamus indicates that the reduction in the amount of neurons induced by neonatal handling, as shown by other studies, is not a general phenomenon in the brain. |
id |
UFRGS-2_e09a06a206f6369e2c64c2dd09d402d9 |
---|---|
oai_identifier_str |
oai:www.lume.ufrgs.br:10183/21129 |
network_acronym_str |
UFRGS-2 |
network_name_str |
Repositório Institucional da UFRGS |
repository_id_str |
|
spelling |
Hermel, Erica do Espirito SantoSeverino, Gabriela SentenaCecconello, Ana LúciaPereira, Francine MartinsSanvitto, Gilberto LuizLucion, Aldo Bolten2010-04-24T04:15:26Z20010100-879Xhttp://hdl.handle.net/10183/21129000324845Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotinperoxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were counted bilaterally in the arcuate, paraventricular and periventricular nuclei of the hypothalamus in 30-μm sections at 120-μm intervals. Neonatal handling did not change the number of TH-IR neurons in the arcuate (1021 ± 206, N = 6; 1020 ± 150, N = 6; nonhandled and handled, respectively), paraventricular (584 ± 85, N = 8; 682 ± 62, N = 9) or periventricular (743 ± 118, N = 7; 990 ± 158, N = 7) nuclei of the hypothalamus. The absence of an effect on the number of dopaminergic cells in the hypothalamus indicates that the reduction in the amount of neurons induced by neonatal handling, as shown by other studies, is not a general phenomenon in the brain.application/pdfengBrazilian journal of medical and biological research =Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 9 (Sept. 2001), p. 1191-1195FisiologiaStressNeonatal stimulationDopamineImmunohistochemistryHypothalamusTyrosine hydroxylaseNeonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male ratsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000324845.pdf000324845.pdfTexto completo (inglês)application/pdf25451http://www.lume.ufrgs.br/bitstream/10183/21129/1/000324845.pdf251450c3f02e0b3a22a96f63804a5668MD51TEXT000324845.pdf.txt000324845.pdf.txtExtracted Texttext/plain16807http://www.lume.ufrgs.br/bitstream/10183/21129/2/000324845.pdf.txt9fdd6d95124ada67e661e0c28960acd0MD52THUMBNAIL000324845.pdf.jpg000324845.pdf.jpgGenerated Thumbnailimage/jpeg1732http://www.lume.ufrgs.br/bitstream/10183/21129/3/000324845.pdf.jpg3be30f2731b9574cb795c10c221d646aMD5310183/211292021-11-20 05:41:35.404412oai:www.lume.ufrgs.br:10183/21129Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T07:41:35Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats |
title |
Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats |
spellingShingle |
Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats Hermel, Erica do Espirito Santo Fisiologia Stress Neonatal stimulation Dopamine Immunohistochemistry Hypothalamus Tyrosine hydroxylase |
title_short |
Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats |
title_full |
Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats |
title_fullStr |
Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats |
title_full_unstemmed |
Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats |
title_sort |
Neonatal handling and the expression of immunoreactivity to tyrosine hydroxylase in the hypothalamus of adult male rats |
author |
Hermel, Erica do Espirito Santo |
author_facet |
Hermel, Erica do Espirito Santo Severino, Gabriela Sentena Cecconello, Ana Lúcia Pereira, Francine Martins Sanvitto, Gilberto Luiz Lucion, Aldo Bolten |
author_role |
author |
author2 |
Severino, Gabriela Sentena Cecconello, Ana Lúcia Pereira, Francine Martins Sanvitto, Gilberto Luiz Lucion, Aldo Bolten |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Hermel, Erica do Espirito Santo Severino, Gabriela Sentena Cecconello, Ana Lúcia Pereira, Francine Martins Sanvitto, Gilberto Luiz Lucion, Aldo Bolten |
dc.subject.por.fl_str_mv |
Fisiologia |
topic |
Fisiologia Stress Neonatal stimulation Dopamine Immunohistochemistry Hypothalamus Tyrosine hydroxylase |
dc.subject.eng.fl_str_mv |
Stress Neonatal stimulation Dopamine Immunohistochemistry Hypothalamus Tyrosine hydroxylase |
description |
Neonatal handling has long-lasting effects on behavior and stress reactivity. The purpose of the present study was to investigate the effect of neonatal handling on the number of dopaminergic neurons in the hypothalamic nuclei of adult male rats as part of a series of studies that could explain the long-lasting effects of neonatal stimulation. Two groups of Wistar rats were studied: nonhandled (pups were left undisturbed, control) and handled (pups were handled for 1 min once a day during the first 10 days of life). At 75-80 days, the males were anesthetized and the brains were processed for immunohistochemistry. An anti-tyrosine hydroxylase antibody and the avidin-biotinperoxidase method were used. Tyrosine hydroxylase-immunoreactive (TH-IR) neurons were counted bilaterally in the arcuate, paraventricular and periventricular nuclei of the hypothalamus in 30-μm sections at 120-μm intervals. Neonatal handling did not change the number of TH-IR neurons in the arcuate (1021 ± 206, N = 6; 1020 ± 150, N = 6; nonhandled and handled, respectively), paraventricular (584 ± 85, N = 8; 682 ± 62, N = 9) or periventricular (743 ± 118, N = 7; 990 ± 158, N = 7) nuclei of the hypothalamus. The absence of an effect on the number of dopaminergic cells in the hypothalamus indicates that the reduction in the amount of neurons induced by neonatal handling, as shown by other studies, is not a general phenomenon in the brain. |
publishDate |
2001 |
dc.date.issued.fl_str_mv |
2001 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:26Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/21129 |
dc.identifier.issn.pt_BR.fl_str_mv |
0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000324845 |
identifier_str_mv |
0100-879X 000324845 |
url |
http://hdl.handle.net/10183/21129 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research =Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 34, no. 9 (Sept. 2001), p. 1191-1195 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
instname_str |
Universidade Federal do Rio Grande do Sul (UFRGS) |
instacron_str |
UFRGS |
institution |
UFRGS |
reponame_str |
Repositório Institucional da UFRGS |
collection |
Repositório Institucional da UFRGS |
bitstream.url.fl_str_mv |
http://www.lume.ufrgs.br/bitstream/10183/21129/1/000324845.pdf http://www.lume.ufrgs.br/bitstream/10183/21129/2/000324845.pdf.txt http://www.lume.ufrgs.br/bitstream/10183/21129/3/000324845.pdf.jpg |
bitstream.checksum.fl_str_mv |
251450c3f02e0b3a22a96f63804a5668 9fdd6d95124ada67e661e0c28960acd0 3be30f2731b9574cb795c10c221d646a |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS) |
repository.mail.fl_str_mv |
|
_version_ |
1815447406583480320 |