Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli

Detalhes bibliográficos
Autor(a) principal: Memmert, Svenja
Data de Publicação: 2019
Outros Autores: Damanaki, Anna, Nogueira, Andressa V. B. [UNESP], Nokhbehsaim, Marjan, Götz, Werner, Cirelli, Joni A. [UNESP], Rath-Deschner, Birgit, Jäger, Andreas, Deschner, James
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00441-018-2941-8
http://hdl.handle.net/11449/188304
Resumo: Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of catecholamines and has been connected to aggravated progression of periodontal disease under chronic stress. Obesity is known to increase the risk of periodontitis and adipokines have been suggested to be a pathomechanistic link. This study examines if obesity-associated stimuli have regulatory effects on TH levels in periodontal cells and tissues. Human periodontal ligament fibroblasts were cultured in the presence of leptin or visfatin for up to 2 days. Untreated cells served as control. TH regulation was analyzed by real-time PCR, immunocytochemistry and ELISA. TH gene expression in periodontal tissues of normal-weight and obese rodents was determined. Examination of gingival biopsies from rats and patients with and without periodontal disease was performed by real-time PCR or immunohistochemistry. For statistics, ANOVA and post hoc tests were applied (p < 0.05). In vitro, TH gene expression and protein levels were increased by leptin and visfatin. In vivo, TH gene expression was upregulated in periodontal tissues of obese rodents as compared to normal-weight animals. Additionally, increased TH gene expression was found in rat gingival biopsies with experimental periodontitis. Human gingival biopsies from sites of periodontitis confirmed the animal data by demonstrating elevated TH levels at periodontally diseased sites. This study provides original evidence that obesity-associated stimuli induce a TH upregulation in periodontal cells and tissues. Since TH levels were also increased at periodontitis sites, our in vitro and animal findings suggest that this enzyme could represent a pathomechanism whereby obesity contributes to periodontitis.
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spelling Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuliAdipokinesObesityPeriodontal ligamentPeriodontitisTyrosine hydroxylaseTyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of catecholamines and has been connected to aggravated progression of periodontal disease under chronic stress. Obesity is known to increase the risk of periodontitis and adipokines have been suggested to be a pathomechanistic link. This study examines if obesity-associated stimuli have regulatory effects on TH levels in periodontal cells and tissues. Human periodontal ligament fibroblasts were cultured in the presence of leptin or visfatin for up to 2 days. Untreated cells served as control. TH regulation was analyzed by real-time PCR, immunocytochemistry and ELISA. TH gene expression in periodontal tissues of normal-weight and obese rodents was determined. Examination of gingival biopsies from rats and patients with and without periodontal disease was performed by real-time PCR or immunohistochemistry. For statistics, ANOVA and post hoc tests were applied (p < 0.05). In vitro, TH gene expression and protein levels were increased by leptin and visfatin. In vivo, TH gene expression was upregulated in periodontal tissues of obese rodents as compared to normal-weight animals. Additionally, increased TH gene expression was found in rat gingival biopsies with experimental periodontitis. Human gingival biopsies from sites of periodontitis confirmed the animal data by demonstrating elevated TH levels at periodontally diseased sites. This study provides original evidence that obesity-associated stimuli induce a TH upregulation in periodontal cells and tissues. Since TH levels were also increased at periodontitis sites, our in vitro and animal findings suggest that this enzyme could represent a pathomechanism whereby obesity contributes to periodontitis.Deutsche ForschungsgemeinschaftDepartment of Orthodontics Center of Dento-Maxillo-Facial Medicine University of Bonn, Welschnonnenstr. 17Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of BonnDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg Univeristy MainzDepartment of Diagnosis and Surgery School of Dentistry at Araraquara University Estadual Paulista—UNESPDepartment of Diagnosis and Surgery School of Dentistry at Araraquara University Estadual Paulista—UNESPDeutsche Forschungsgemeinschaft: ME 4798/1-1University of BonnUniversity Medical Center of the Johannes Gutenberg Univeristy MainzUniversidade Estadual Paulista (Unesp)Memmert, SvenjaDamanaki, AnnaNogueira, Andressa V. B. [UNESP]Nokhbehsaim, MarjanGötz, WernerCirelli, Joni A. [UNESP]Rath-Deschner, BirgitJäger, AndreasDeschner, James2019-10-06T16:03:45Z2019-10-06T16:03:45Z2019-03-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article619-628http://dx.doi.org/10.1007/s00441-018-2941-8Cell and Tissue Research, v. 375, n. 3, p. 619-628, 2019.1432-08780302-766Xhttp://hdl.handle.net/11449/18830410.1007/s00441-018-2941-82-s2.0-85055878800Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCell and Tissue Researchinfo:eu-repo/semantics/openAccess2021-10-23T19:23:45Zoai:repositorio.unesp.br:11449/188304Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
title Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
spellingShingle Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
Memmert, Svenja
Adipokines
Obesity
Periodontal ligament
Periodontitis
Tyrosine hydroxylase
title_short Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
title_full Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
title_fullStr Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
title_full_unstemmed Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
title_sort Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
author Memmert, Svenja
author_facet Memmert, Svenja
Damanaki, Anna
Nogueira, Andressa V. B. [UNESP]
Nokhbehsaim, Marjan
Götz, Werner
Cirelli, Joni A. [UNESP]
Rath-Deschner, Birgit
Jäger, Andreas
Deschner, James
author_role author
author2 Damanaki, Anna
Nogueira, Andressa V. B. [UNESP]
Nokhbehsaim, Marjan
Götz, Werner
Cirelli, Joni A. [UNESP]
Rath-Deschner, Birgit
Jäger, Andreas
Deschner, James
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University of Bonn
University Medical Center of the Johannes Gutenberg Univeristy Mainz
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Memmert, Svenja
Damanaki, Anna
Nogueira, Andressa V. B. [UNESP]
Nokhbehsaim, Marjan
Götz, Werner
Cirelli, Joni A. [UNESP]
Rath-Deschner, Birgit
Jäger, Andreas
Deschner, James
dc.subject.por.fl_str_mv Adipokines
Obesity
Periodontal ligament
Periodontitis
Tyrosine hydroxylase
topic Adipokines
Obesity
Periodontal ligament
Periodontitis
Tyrosine hydroxylase
description Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of catecholamines and has been connected to aggravated progression of periodontal disease under chronic stress. Obesity is known to increase the risk of periodontitis and adipokines have been suggested to be a pathomechanistic link. This study examines if obesity-associated stimuli have regulatory effects on TH levels in periodontal cells and tissues. Human periodontal ligament fibroblasts were cultured in the presence of leptin or visfatin for up to 2 days. Untreated cells served as control. TH regulation was analyzed by real-time PCR, immunocytochemistry and ELISA. TH gene expression in periodontal tissues of normal-weight and obese rodents was determined. Examination of gingival biopsies from rats and patients with and without periodontal disease was performed by real-time PCR or immunohistochemistry. For statistics, ANOVA and post hoc tests were applied (p < 0.05). In vitro, TH gene expression and protein levels were increased by leptin and visfatin. In vivo, TH gene expression was upregulated in periodontal tissues of obese rodents as compared to normal-weight animals. Additionally, increased TH gene expression was found in rat gingival biopsies with experimental periodontitis. Human gingival biopsies from sites of periodontitis confirmed the animal data by demonstrating elevated TH levels at periodontally diseased sites. This study provides original evidence that obesity-associated stimuli induce a TH upregulation in periodontal cells and tissues. Since TH levels were also increased at periodontitis sites, our in vitro and animal findings suggest that this enzyme could represent a pathomechanism whereby obesity contributes to periodontitis.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:03:45Z
2019-10-06T16:03:45Z
2019-03-05
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00441-018-2941-8
Cell and Tissue Research, v. 375, n. 3, p. 619-628, 2019.
1432-0878
0302-766X
http://hdl.handle.net/11449/188304
10.1007/s00441-018-2941-8
2-s2.0-85055878800
url http://dx.doi.org/10.1007/s00441-018-2941-8
http://hdl.handle.net/11449/188304
identifier_str_mv Cell and Tissue Research, v. 375, n. 3, p. 619-628, 2019.
1432-0878
0302-766X
10.1007/s00441-018-2941-8
2-s2.0-85055878800
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cell and Tissue Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 619-628
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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