Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00441-018-2941-8 http://hdl.handle.net/11449/188304 |
Resumo: | Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of catecholamines and has been connected to aggravated progression of periodontal disease under chronic stress. Obesity is known to increase the risk of periodontitis and adipokines have been suggested to be a pathomechanistic link. This study examines if obesity-associated stimuli have regulatory effects on TH levels in periodontal cells and tissues. Human periodontal ligament fibroblasts were cultured in the presence of leptin or visfatin for up to 2 days. Untreated cells served as control. TH regulation was analyzed by real-time PCR, immunocytochemistry and ELISA. TH gene expression in periodontal tissues of normal-weight and obese rodents was determined. Examination of gingival biopsies from rats and patients with and without periodontal disease was performed by real-time PCR or immunohistochemistry. For statistics, ANOVA and post hoc tests were applied (p < 0.05). In vitro, TH gene expression and protein levels were increased by leptin and visfatin. In vivo, TH gene expression was upregulated in periodontal tissues of obese rodents as compared to normal-weight animals. Additionally, increased TH gene expression was found in rat gingival biopsies with experimental periodontitis. Human gingival biopsies from sites of periodontitis confirmed the animal data by demonstrating elevated TH levels at periodontally diseased sites. This study provides original evidence that obesity-associated stimuli induce a TH upregulation in periodontal cells and tissues. Since TH levels were also increased at periodontitis sites, our in vitro and animal findings suggest that this enzyme could represent a pathomechanism whereby obesity contributes to periodontitis. |
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Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuliAdipokinesObesityPeriodontal ligamentPeriodontitisTyrosine hydroxylaseTyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of catecholamines and has been connected to aggravated progression of periodontal disease under chronic stress. Obesity is known to increase the risk of periodontitis and adipokines have been suggested to be a pathomechanistic link. This study examines if obesity-associated stimuli have regulatory effects on TH levels in periodontal cells and tissues. Human periodontal ligament fibroblasts were cultured in the presence of leptin or visfatin for up to 2 days. Untreated cells served as control. TH regulation was analyzed by real-time PCR, immunocytochemistry and ELISA. TH gene expression in periodontal tissues of normal-weight and obese rodents was determined. Examination of gingival biopsies from rats and patients with and without periodontal disease was performed by real-time PCR or immunohistochemistry. For statistics, ANOVA and post hoc tests were applied (p < 0.05). In vitro, TH gene expression and protein levels were increased by leptin and visfatin. In vivo, TH gene expression was upregulated in periodontal tissues of obese rodents as compared to normal-weight animals. Additionally, increased TH gene expression was found in rat gingival biopsies with experimental periodontitis. Human gingival biopsies from sites of periodontitis confirmed the animal data by demonstrating elevated TH levels at periodontally diseased sites. This study provides original evidence that obesity-associated stimuli induce a TH upregulation in periodontal cells and tissues. Since TH levels were also increased at periodontitis sites, our in vitro and animal findings suggest that this enzyme could represent a pathomechanism whereby obesity contributes to periodontitis.Deutsche ForschungsgemeinschaftDepartment of Orthodontics Center of Dento-Maxillo-Facial Medicine University of Bonn, Welschnonnenstr. 17Section of Experimental Dento-Maxillo-Facial Medicine Center of Dento-Maxillo-Facial Medicine University of BonnDepartment of Periodontology and Operative Dentistry University Medical Center of the Johannes Gutenberg Univeristy MainzDepartment of Diagnosis and Surgery School of Dentistry at Araraquara University Estadual Paulista—UNESPDepartment of Diagnosis and Surgery School of Dentistry at Araraquara University Estadual Paulista—UNESPDeutsche Forschungsgemeinschaft: ME 4798/1-1University of BonnUniversity Medical Center of the Johannes Gutenberg Univeristy MainzUniversidade Estadual Paulista (Unesp)Memmert, SvenjaDamanaki, AnnaNogueira, Andressa V. B. [UNESP]Nokhbehsaim, MarjanGötz, WernerCirelli, Joni A. [UNESP]Rath-Deschner, BirgitJäger, AndreasDeschner, James2019-10-06T16:03:45Z2019-10-06T16:03:45Z2019-03-05info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article619-628http://dx.doi.org/10.1007/s00441-018-2941-8Cell and Tissue Research, v. 375, n. 3, p. 619-628, 2019.1432-08780302-766Xhttp://hdl.handle.net/11449/18830410.1007/s00441-018-2941-82-s2.0-85055878800Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCell and Tissue Researchinfo:eu-repo/semantics/openAccess2021-10-23T19:23:45Zoai:repositorio.unesp.br:11449/188304Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T19:23:45Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli |
title |
Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli |
spellingShingle |
Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli Memmert, Svenja Adipokines Obesity Periodontal ligament Periodontitis Tyrosine hydroxylase |
title_short |
Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli |
title_full |
Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli |
title_fullStr |
Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli |
title_full_unstemmed |
Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli |
title_sort |
Regulation of tyrosine hydroxylase in periodontal fibroblasts and tissues by obesity-associated stimuli |
author |
Memmert, Svenja |
author_facet |
Memmert, Svenja Damanaki, Anna Nogueira, Andressa V. B. [UNESP] Nokhbehsaim, Marjan Götz, Werner Cirelli, Joni A. [UNESP] Rath-Deschner, Birgit Jäger, Andreas Deschner, James |
author_role |
author |
author2 |
Damanaki, Anna Nogueira, Andressa V. B. [UNESP] Nokhbehsaim, Marjan Götz, Werner Cirelli, Joni A. [UNESP] Rath-Deschner, Birgit Jäger, Andreas Deschner, James |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Bonn University Medical Center of the Johannes Gutenberg Univeristy Mainz Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Memmert, Svenja Damanaki, Anna Nogueira, Andressa V. B. [UNESP] Nokhbehsaim, Marjan Götz, Werner Cirelli, Joni A. [UNESP] Rath-Deschner, Birgit Jäger, Andreas Deschner, James |
dc.subject.por.fl_str_mv |
Adipokines Obesity Periodontal ligament Periodontitis Tyrosine hydroxylase |
topic |
Adipokines Obesity Periodontal ligament Periodontitis Tyrosine hydroxylase |
description |
Tyrosine hydroxylase (TH) catalyzes the rate-limiting step in the synthesis of catecholamines and has been connected to aggravated progression of periodontal disease under chronic stress. Obesity is known to increase the risk of periodontitis and adipokines have been suggested to be a pathomechanistic link. This study examines if obesity-associated stimuli have regulatory effects on TH levels in periodontal cells and tissues. Human periodontal ligament fibroblasts were cultured in the presence of leptin or visfatin for up to 2 days. Untreated cells served as control. TH regulation was analyzed by real-time PCR, immunocytochemistry and ELISA. TH gene expression in periodontal tissues of normal-weight and obese rodents was determined. Examination of gingival biopsies from rats and patients with and without periodontal disease was performed by real-time PCR or immunohistochemistry. For statistics, ANOVA and post hoc tests were applied (p < 0.05). In vitro, TH gene expression and protein levels were increased by leptin and visfatin. In vivo, TH gene expression was upregulated in periodontal tissues of obese rodents as compared to normal-weight animals. Additionally, increased TH gene expression was found in rat gingival biopsies with experimental periodontitis. Human gingival biopsies from sites of periodontitis confirmed the animal data by demonstrating elevated TH levels at periodontally diseased sites. This study provides original evidence that obesity-associated stimuli induce a TH upregulation in periodontal cells and tissues. Since TH levels were also increased at periodontitis sites, our in vitro and animal findings suggest that this enzyme could represent a pathomechanism whereby obesity contributes to periodontitis. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:03:45Z 2019-10-06T16:03:45Z 2019-03-05 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00441-018-2941-8 Cell and Tissue Research, v. 375, n. 3, p. 619-628, 2019. 1432-0878 0302-766X http://hdl.handle.net/11449/188304 10.1007/s00441-018-2941-8 2-s2.0-85055878800 |
url |
http://dx.doi.org/10.1007/s00441-018-2941-8 http://hdl.handle.net/11449/188304 |
identifier_str_mv |
Cell and Tissue Research, v. 375, n. 3, p. 619-628, 2019. 1432-0878 0302-766X 10.1007/s00441-018-2941-8 2-s2.0-85055878800 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cell and Tissue Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
619-628 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965115026505728 |