MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/242315 |
Resumo: | Introduction: Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR- 15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR- 15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation. Methods: MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software. Results: MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia. Conclusion: MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD. |
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Dieter, CristineAssmann, Taís SilveiraCosta, Aline RodriguesCanani, Luis Henrique SantosSouza, Bianca Marmontel deBauer, Andrea CarlaCrispim, Daisy2022-07-13T04:53:09Z20191664-8021http://hdl.handle.net/10183/242315001143528Introduction: Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR- 15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR- 15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation. Methods: MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software. Results: MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia. Conclusion: MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD.application/pdfengFrontiers in genetics. Lausanne. Vol. 10, 563, 12 p.MicroRNAsNefropatias diabéticasBiologia computacionalDiabetes mellitus tipo 1MicroRNA expressionMiR-15a-5pMiR-30e-5pDiabetic kidney diseaseBioinformatics analysisType 1 diabetes mellitusMiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney diseaseEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001143528.pdf.txt001143528.pdf.txtExtracted Texttext/plain54059http://www.lume.ufrgs.br/bitstream/10183/242315/2/001143528.pdf.txt04b88f94008a221748f545bf16029c6cMD52ORIGINAL001143528.pdfTexto completo (inglês)application/pdf1640091http://www.lume.ufrgs.br/bitstream/10183/242315/1/001143528.pdfcc8f5bae80bcd6a1372a3e7e36a263dfMD5110183/2423152023-01-22 05:58:22.151147oai:www.lume.ufrgs.br:10183/242315Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2023-01-22T07:58:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease |
title |
MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease |
spellingShingle |
MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease Dieter, Cristine MicroRNAs Nefropatias diabéticas Biologia computacional Diabetes mellitus tipo 1 MicroRNA expression MiR-15a-5p MiR-30e-5p Diabetic kidney disease Bioinformatics analysis Type 1 diabetes mellitus |
title_short |
MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease |
title_full |
MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease |
title_fullStr |
MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease |
title_full_unstemmed |
MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease |
title_sort |
MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease |
author |
Dieter, Cristine |
author_facet |
Dieter, Cristine Assmann, Taís Silveira Costa, Aline Rodrigues Canani, Luis Henrique Santos Souza, Bianca Marmontel de Bauer, Andrea Carla Crispim, Daisy |
author_role |
author |
author2 |
Assmann, Taís Silveira Costa, Aline Rodrigues Canani, Luis Henrique Santos Souza, Bianca Marmontel de Bauer, Andrea Carla Crispim, Daisy |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Dieter, Cristine Assmann, Taís Silveira Costa, Aline Rodrigues Canani, Luis Henrique Santos Souza, Bianca Marmontel de Bauer, Andrea Carla Crispim, Daisy |
dc.subject.por.fl_str_mv |
MicroRNAs Nefropatias diabéticas Biologia computacional Diabetes mellitus tipo 1 |
topic |
MicroRNAs Nefropatias diabéticas Biologia computacional Diabetes mellitus tipo 1 MicroRNA expression MiR-15a-5p MiR-30e-5p Diabetic kidney disease Bioinformatics analysis Type 1 diabetes mellitus |
dc.subject.eng.fl_str_mv |
MicroRNA expression MiR-15a-5p MiR-30e-5p Diabetic kidney disease Bioinformatics analysis Type 1 diabetes mellitus |
description |
Introduction: Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR- 15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR- 15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation. Methods: MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software. Results: MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia. Conclusion: MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2022-07-13T04:53:09Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
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http://hdl.handle.net/10183/242315 |
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1664-8021 |
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001143528 |
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http://hdl.handle.net/10183/242315 |
dc.language.iso.fl_str_mv |
eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Frontiers in genetics. Lausanne. Vol. 10, 563, 12 p. |
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