MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease

Detalhes bibliográficos
Autor(a) principal: Dieter, Cristine
Data de Publicação: 2019
Outros Autores: Assmann, Taís Silveira, Costa, Aline Rodrigues, Canani, Luis Henrique Santos, Souza, Bianca Marmontel de, Bauer, Andrea Carla, Crispim, Daisy
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/242315
Resumo: Introduction: Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR- 15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR- 15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation. Methods: MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software. Results: MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia. Conclusion: MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD.
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spelling Dieter, CristineAssmann, Taís SilveiraCosta, Aline RodriguesCanani, Luis Henrique SantosSouza, Bianca Marmontel deBauer, Andrea CarlaCrispim, Daisy2022-07-13T04:53:09Z20191664-8021http://hdl.handle.net/10183/242315001143528Introduction: Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR- 15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR- 15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation. Methods: MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software. Results: MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia. Conclusion: MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD.application/pdfengFrontiers in genetics. Lausanne. Vol. 10, 563, 12 p.MicroRNAsNefropatias diabéticasBiologia computacionalDiabetes mellitus tipo 1MicroRNA expressionMiR-15a-5pMiR-30e-5pDiabetic kidney diseaseBioinformatics analysisType 1 diabetes mellitusMiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney diseaseEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001143528.pdf.txt001143528.pdf.txtExtracted Texttext/plain54059http://www.lume.ufrgs.br/bitstream/10183/242315/2/001143528.pdf.txt04b88f94008a221748f545bf16029c6cMD52ORIGINAL001143528.pdfTexto completo (inglês)application/pdf1640091http://www.lume.ufrgs.br/bitstream/10183/242315/1/001143528.pdfcc8f5bae80bcd6a1372a3e7e36a263dfMD5110183/2423152023-01-22 05:58:22.151147oai:www.lume.ufrgs.br:10183/242315Repositório InstitucionalPUBhttps://lume.ufrgs.br/oai/requestlume@ufrgs.bropendoar:2023-01-22T07:58:22Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
title MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
spellingShingle MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
Dieter, Cristine
MicroRNAs
Nefropatias diabéticas
Biologia computacional
Diabetes mellitus tipo 1
MicroRNA expression
MiR-15a-5p
MiR-30e-5p
Diabetic kidney disease
Bioinformatics analysis
Type 1 diabetes mellitus
title_short MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
title_full MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
title_fullStr MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
title_full_unstemmed MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
title_sort MiR-30e-5p and miR-15a-5p expressions in plasma and urine of type 1 diabetic patients with diabetic kidney disease
author Dieter, Cristine
author_facet Dieter, Cristine
Assmann, Taís Silveira
Costa, Aline Rodrigues
Canani, Luis Henrique Santos
Souza, Bianca Marmontel de
Bauer, Andrea Carla
Crispim, Daisy
author_role author
author2 Assmann, Taís Silveira
Costa, Aline Rodrigues
Canani, Luis Henrique Santos
Souza, Bianca Marmontel de
Bauer, Andrea Carla
Crispim, Daisy
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dieter, Cristine
Assmann, Taís Silveira
Costa, Aline Rodrigues
Canani, Luis Henrique Santos
Souza, Bianca Marmontel de
Bauer, Andrea Carla
Crispim, Daisy
dc.subject.por.fl_str_mv MicroRNAs
Nefropatias diabéticas
Biologia computacional
Diabetes mellitus tipo 1
topic MicroRNAs
Nefropatias diabéticas
Biologia computacional
Diabetes mellitus tipo 1
MicroRNA expression
MiR-15a-5p
MiR-30e-5p
Diabetic kidney disease
Bioinformatics analysis
Type 1 diabetes mellitus
dc.subject.eng.fl_str_mv MicroRNA expression
MiR-15a-5p
MiR-30e-5p
Diabetic kidney disease
Bioinformatics analysis
Type 1 diabetes mellitus
description Introduction: Diabetic kidney disease (DKD) is a common microvascular complication that affects 40% of patients with diabetes mellitus (DM). Emerging evidence suggests a role for several microRNAs (miRNAs) in the development of DKD. In this context, miR- 15a-5p and miR-30e-5p have been shown to regulate the expression of the uncoupling protein 2 (UCP2), a mitochondrial protein that decreases reactive oxygen species (ROS) formation by the mitochondria. Since ROS overproduction is a key contributor to the pathogenesis of DKD, dysregulation of these two miRNAs could be involved in DKD pathogenesis. Thus, the aim of this study was to compare the expressions of miR- 15a-5p and miR-30e-5p in type 1 DM (T1DM) patients with DKD (cases) and without this complication (controls), and to perform bioinformatics analyses to investigate their putative targets and biological pathways under their regulation. Methods: MiR-15a-5p and miR-30e-5p expressions were analyzed in plasma and urine of 17 T1DM controls and 23 DKD cases (12 with moderate DKD and 11 with severe DKD) using qPCR. Bioinformatics analyses were performed in Cytoscape software. Results: MiR-30e-5p expression was downregulated in plasma of patients with moderate and severe DKD compared to T1DM controls. Moreover, this miRNA was also downregulated in urine of patients with severe DKD compared to the other groups. No difference was found in miR-15a-5p expression between groups. Bioinformatics analyses indicated that miR-30e-5p and miR-15a-5p regulate various genes that participate in pathways related to angiogenesis, apoptosis, cell differentiation, oxidative stress, and hypoxia. Conclusion: MiR-30e-5p seems to be downregulated in plasma and urine of patients with DKD.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2022-07-13T04:53:09Z
dc.type.driver.fl_str_mv Estrangeiro
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dc.identifier.issn.pt_BR.fl_str_mv 1664-8021
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dc.relation.ispartof.pt_BR.fl_str_mv Frontiers in genetics. Lausanne. Vol. 10, 563, 12 p.
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