Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/204387 |
Resumo: | MicroRNAs (miRNAs/miRs) are involved in the pathogenesis of diabetes mellitus and its chronic complications, and their circulating levels have emerged as potential biomarkers for the development and progression of diabetes. However, few studies have examined the expression of miRNAs in diabetic retinopathy (DR) in humans. This case-control study aimed to investigate whether the plasma levels of miR-29b and miR-200b are associated with DR in 186 South Brazilians with type 2 diabetes (91 without DR, 46 with non-proliferative DR and 49 with proliferative DR). We also included 20 healthy blood donors to determine the miRNA expression in the general population. Plasma levels of miR-29b and miR-200b were quantified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Proliferative DR was inversely associated with plasma levels of miR-29b (unadjusted OR = 0.694, 95% CI: 0.535-0.900, P = 0.006) and miR-200b (unadjusted OR = 0.797, 95% CI: 0.637-0.997, P = 0.047). However, these associations were lost after controlling for demographic and clinical covariates. In addition, patients with type 2 diabetes had lower miR-200b levels than blood donors. Our findings reinforce the importance of addressing the role of circulating miRNAs, including miR-29 and miR-200b, in DR. |
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Silva, Maria Enoia Dantas da Costa ePolina, Evelise ReginaCrispim, DaisySbruzzi, Renan CesarLavinsky, DanielMallmann, FelipeMartinelli, Nidiane CarlaCanani, Luis Henrique SantosSantos, Kátia Gonçalves dos2020-01-16T04:10:34Z20191582-4934http://hdl.handle.net/10183/204387001107223MicroRNAs (miRNAs/miRs) are involved in the pathogenesis of diabetes mellitus and its chronic complications, and their circulating levels have emerged as potential biomarkers for the development and progression of diabetes. However, few studies have examined the expression of miRNAs in diabetic retinopathy (DR) in humans. This case-control study aimed to investigate whether the plasma levels of miR-29b and miR-200b are associated with DR in 186 South Brazilians with type 2 diabetes (91 without DR, 46 with non-proliferative DR and 49 with proliferative DR). We also included 20 healthy blood donors to determine the miRNA expression in the general population. Plasma levels of miR-29b and miR-200b were quantified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Proliferative DR was inversely associated with plasma levels of miR-29b (unadjusted OR = 0.694, 95% CI: 0.535-0.900, P = 0.006) and miR-200b (unadjusted OR = 0.797, 95% CI: 0.637-0.997, P = 0.047). However, these associations were lost after controlling for demographic and clinical covariates. In addition, patients with type 2 diabetes had lower miR-200b levels than blood donors. Our findings reinforce the importance of addressing the role of circulating miRNAs, including miR-29 and miR-200b, in DR.application/pdfengJournal of cellular and molecular medicine. Oxford. Vol. 23 (2019), p. 1280-1287Diabetes mellitus tipo 2MicroRNAsExpressão gênicaRetinopatia diabéticaReação em cadeia da polimerase via transcriptase reversaEpigenômicaEnsaio clínico controladoCirculating levelsDiabetic retinopathyEpigeneticsGene expressionMicroRNAType 2 diabetes mellitusPlasma levels of miR-29b and miR-200b in type 2 diabetic retinopathyEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001107223.pdf.txt001107223.pdf.txtExtracted Texttext/plain44171http://www.lume.ufrgs.br/bitstream/10183/204387/2/001107223.pdf.txtac524a9886e1a02e1b23ace80efe4debMD52ORIGINAL001107223.pdfTexto completo (inglês)application/pdf200530http://www.lume.ufrgs.br/bitstream/10183/204387/1/001107223.pdfa78ff0d7b1576a189043e47ab7b62dabMD5110183/2043872023-05-24 03:28:59.109276oai:www.lume.ufrgs.br:10183/204387Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2023-05-24T06:28:59Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy |
title |
Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy |
spellingShingle |
Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy Silva, Maria Enoia Dantas da Costa e Diabetes mellitus tipo 2 MicroRNAs Expressão gênica Retinopatia diabética Reação em cadeia da polimerase via transcriptase reversa Epigenômica Ensaio clínico controlado Circulating levels Diabetic retinopathy Epigenetics Gene expression MicroRNA Type 2 diabetes mellitus |
title_short |
Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy |
title_full |
Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy |
title_fullStr |
Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy |
title_full_unstemmed |
Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy |
title_sort |
Plasma levels of miR-29b and miR-200b in type 2 diabetic retinopathy |
author |
Silva, Maria Enoia Dantas da Costa e |
author_facet |
Silva, Maria Enoia Dantas da Costa e Polina, Evelise Regina Crispim, Daisy Sbruzzi, Renan Cesar Lavinsky, Daniel Mallmann, Felipe Martinelli, Nidiane Carla Canani, Luis Henrique Santos Santos, Kátia Gonçalves dos |
author_role |
author |
author2 |
Polina, Evelise Regina Crispim, Daisy Sbruzzi, Renan Cesar Lavinsky, Daniel Mallmann, Felipe Martinelli, Nidiane Carla Canani, Luis Henrique Santos Santos, Kátia Gonçalves dos |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Silva, Maria Enoia Dantas da Costa e Polina, Evelise Regina Crispim, Daisy Sbruzzi, Renan Cesar Lavinsky, Daniel Mallmann, Felipe Martinelli, Nidiane Carla Canani, Luis Henrique Santos Santos, Kátia Gonçalves dos |
dc.subject.por.fl_str_mv |
Diabetes mellitus tipo 2 MicroRNAs Expressão gênica Retinopatia diabética Reação em cadeia da polimerase via transcriptase reversa Epigenômica Ensaio clínico controlado |
topic |
Diabetes mellitus tipo 2 MicroRNAs Expressão gênica Retinopatia diabética Reação em cadeia da polimerase via transcriptase reversa Epigenômica Ensaio clínico controlado Circulating levels Diabetic retinopathy Epigenetics Gene expression MicroRNA Type 2 diabetes mellitus |
dc.subject.eng.fl_str_mv |
Circulating levels Diabetic retinopathy Epigenetics Gene expression MicroRNA Type 2 diabetes mellitus |
description |
MicroRNAs (miRNAs/miRs) are involved in the pathogenesis of diabetes mellitus and its chronic complications, and their circulating levels have emerged as potential biomarkers for the development and progression of diabetes. However, few studies have examined the expression of miRNAs in diabetic retinopathy (DR) in humans. This case-control study aimed to investigate whether the plasma levels of miR-29b and miR-200b are associated with DR in 186 South Brazilians with type 2 diabetes (91 without DR, 46 with non-proliferative DR and 49 with proliferative DR). We also included 20 healthy blood donors to determine the miRNA expression in the general population. Plasma levels of miR-29b and miR-200b were quantified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Proliferative DR was inversely associated with plasma levels of miR-29b (unadjusted OR = 0.694, 95% CI: 0.535-0.900, P = 0.006) and miR-200b (unadjusted OR = 0.797, 95% CI: 0.637-0.997, P = 0.047). However, these associations were lost after controlling for demographic and clinical covariates. In addition, patients with type 2 diabetes had lower miR-200b levels than blood donors. Our findings reinforce the importance of addressing the role of circulating miRNAs, including miR-29 and miR-200b, in DR. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019 |
dc.date.accessioned.fl_str_mv |
2020-01-16T04:10:34Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
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article |
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publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/204387 |
dc.identifier.issn.pt_BR.fl_str_mv |
1582-4934 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001107223 |
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1582-4934 001107223 |
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http://hdl.handle.net/10183/204387 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Journal of cellular and molecular medicine. Oxford. Vol. 23 (2019), p. 1280-1287 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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