In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility

Detalhes bibliográficos
Autor(a) principal: Galia, Carlos Roberto
Data de Publicação: 2008
Outros Autores: Macedo, Carlos Alberto de Souza, Rosito, Ricardo, Mello, Tielle Müller de, Camargo, Lourdes Maria Araújo Quaresma, Moreira, Luis Fernando
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRGS
Texto Completo: http://hdl.handle.net/10183/49785
Resumo: INTRODUCTION: The use of bone grafts in orthopedic, maxillofacial and dental surgery has been growing. Nevertheless, both fresh autografts and frozen allografts have limitations, and therefore, alternative synthetic or natural biomaterials, such as processed and lyophilized bovine bone graft have been developed. OBJECTIVE: To evaluate in vitro and in vivo biocompatibility of lyophilized bovine bone manufactured in a semi-industrial scale, according to a modifical protocol developed by the authors. METHODS: Samples of bovine cancellous bone were processed according to a protocol developed by Kakiuchi et al., and modi¬fied to process samples of bovine cancellous bone. The following trials were performed: in vitro cytotoxicity, in vivo acute systemic toxicity, in vivo oral irritation potential, in vitro pyrogenic reaction, and bioburden. RESULTS: The in vitro evaluation of lyophilized bovine cancellous bone revealed an absence of cytotoxicity in 100% of the samples. Regarding in vivo evaluation of acute systemic toxicity, neither macroscopic abnormalities nor deaths were noted in the animals. Pyrogenicity was not greater than 0.125 UE/ml in any of the samples. The bioburden revealed negative results for microbial growth before sterilization. Regarding the oral irritation potential, in vivo evaluation at 24 and 72 hours showed that the animals had no edema or erythema on the oral mucosa. CONCLUSION: The protocol changes established by the authors to prepare lyophilized bovine cancellous bone at a semi-industrial scale is reproducible and yielded a product with excellent biocompatibility.
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spelling Galia, Carlos RobertoMacedo, Carlos Alberto de SouzaRosito, RicardoMello, Tielle Müller deCamargo, Lourdes Maria Araújo QuaresmaMoreira, Luis Fernando2012-06-27T01:31:57Z20081807-5932http://hdl.handle.net/10183/49785000716537INTRODUCTION: The use of bone grafts in orthopedic, maxillofacial and dental surgery has been growing. Nevertheless, both fresh autografts and frozen allografts have limitations, and therefore, alternative synthetic or natural biomaterials, such as processed and lyophilized bovine bone graft have been developed. OBJECTIVE: To evaluate in vitro and in vivo biocompatibility of lyophilized bovine bone manufactured in a semi-industrial scale, according to a modifical protocol developed by the authors. METHODS: Samples of bovine cancellous bone were processed according to a protocol developed by Kakiuchi et al., and modi¬fied to process samples of bovine cancellous bone. The following trials were performed: in vitro cytotoxicity, in vivo acute systemic toxicity, in vivo oral irritation potential, in vitro pyrogenic reaction, and bioburden. RESULTS: The in vitro evaluation of lyophilized bovine cancellous bone revealed an absence of cytotoxicity in 100% of the samples. Regarding in vivo evaluation of acute systemic toxicity, neither macroscopic abnormalities nor deaths were noted in the animals. Pyrogenicity was not greater than 0.125 UE/ml in any of the samples. The bioburden revealed negative results for microbial growth before sterilization. Regarding the oral irritation potential, in vivo evaluation at 24 and 72 hours showed that the animals had no edema or erythema on the oral mucosa. CONCLUSION: The protocol changes established by the authors to prepare lyophilized bovine cancellous bone at a semi-industrial scale is reproducible and yielded a product with excellent biocompatibility.application/pdfengClinics. São Paulo. Vol. 63, n. 6 (2008), p. 801-806CirurgiaTestes de toxicidadeLiofilizaçãoTransplante ósseoSurgeryMaterials testingToxicity testsFreeze dryingBone transplantationIn vitro and in vivo evaluation of lyophilized bovine bone biocompatibilityinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000716537.pdf000716537.pdfTexto completo (inglês)application/pdf109559http://www.lume.ufrgs.br/bitstream/10183/49785/1/000716537.pdfdf68fc6a6fe3f81643712e2c5b98265dMD51TEXT000716537.pdf.txt000716537.pdf.txtExtracted Texttext/plain28710http://www.lume.ufrgs.br/bitstream/10183/49785/2/000716537.pdf.txtb8811b64472e25c6292fbd521c58e34eMD52THUMBNAIL000716537.pdf.jpg000716537.pdf.jpgGenerated Thumbnailimage/jpeg1834http://www.lume.ufrgs.br/bitstream/10183/49785/3/000716537.pdf.jpgab1e64829b45a3cce3ad50a1b109a87bMD5310183/497852018-10-29 09:26:26.464oai:www.lume.ufrgs.br:10183/49785Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-29T12:26:26Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false
dc.title.pt_BR.fl_str_mv In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
title In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
spellingShingle In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
Galia, Carlos Roberto
Cirurgia
Testes de toxicidade
Liofilização
Transplante ósseo
Surgery
Materials testing
Toxicity tests
Freeze drying
Bone transplantation
title_short In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
title_full In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
title_fullStr In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
title_full_unstemmed In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
title_sort In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
author Galia, Carlos Roberto
author_facet Galia, Carlos Roberto
Macedo, Carlos Alberto de Souza
Rosito, Ricardo
Mello, Tielle Müller de
Camargo, Lourdes Maria Araújo Quaresma
Moreira, Luis Fernando
author_role author
author2 Macedo, Carlos Alberto de Souza
Rosito, Ricardo
Mello, Tielle Müller de
Camargo, Lourdes Maria Araújo Quaresma
Moreira, Luis Fernando
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Galia, Carlos Roberto
Macedo, Carlos Alberto de Souza
Rosito, Ricardo
Mello, Tielle Müller de
Camargo, Lourdes Maria Araújo Quaresma
Moreira, Luis Fernando
dc.subject.por.fl_str_mv Cirurgia
Testes de toxicidade
Liofilização
Transplante ósseo
topic Cirurgia
Testes de toxicidade
Liofilização
Transplante ósseo
Surgery
Materials testing
Toxicity tests
Freeze drying
Bone transplantation
dc.subject.eng.fl_str_mv Surgery
Materials testing
Toxicity tests
Freeze drying
Bone transplantation
description INTRODUCTION: The use of bone grafts in orthopedic, maxillofacial and dental surgery has been growing. Nevertheless, both fresh autografts and frozen allografts have limitations, and therefore, alternative synthetic or natural biomaterials, such as processed and lyophilized bovine bone graft have been developed. OBJECTIVE: To evaluate in vitro and in vivo biocompatibility of lyophilized bovine bone manufactured in a semi-industrial scale, according to a modifical protocol developed by the authors. METHODS: Samples of bovine cancellous bone were processed according to a protocol developed by Kakiuchi et al., and modi¬fied to process samples of bovine cancellous bone. The following trials were performed: in vitro cytotoxicity, in vivo acute systemic toxicity, in vivo oral irritation potential, in vitro pyrogenic reaction, and bioburden. RESULTS: The in vitro evaluation of lyophilized bovine cancellous bone revealed an absence of cytotoxicity in 100% of the samples. Regarding in vivo evaluation of acute systemic toxicity, neither macroscopic abnormalities nor deaths were noted in the animals. Pyrogenicity was not greater than 0.125 UE/ml in any of the samples. The bioburden revealed negative results for microbial growth before sterilization. Regarding the oral irritation potential, in vivo evaluation at 24 and 72 hours showed that the animals had no edema or erythema on the oral mucosa. CONCLUSION: The protocol changes established by the authors to prepare lyophilized bovine cancellous bone at a semi-industrial scale is reproducible and yielded a product with excellent biocompatibility.
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dc.relation.ispartof.pt_BR.fl_str_mv Clinics. São Paulo. Vol. 63, n. 6 (2008), p. 801-806
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