In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/49785 |
Resumo: | INTRODUCTION: The use of bone grafts in orthopedic, maxillofacial and dental surgery has been growing. Nevertheless, both fresh autografts and frozen allografts have limitations, and therefore, alternative synthetic or natural biomaterials, such as processed and lyophilized bovine bone graft have been developed. OBJECTIVE: To evaluate in vitro and in vivo biocompatibility of lyophilized bovine bone manufactured in a semi-industrial scale, according to a modifical protocol developed by the authors. METHODS: Samples of bovine cancellous bone were processed according to a protocol developed by Kakiuchi et al., and modi¬fied to process samples of bovine cancellous bone. The following trials were performed: in vitro cytotoxicity, in vivo acute systemic toxicity, in vivo oral irritation potential, in vitro pyrogenic reaction, and bioburden. RESULTS: The in vitro evaluation of lyophilized bovine cancellous bone revealed an absence of cytotoxicity in 100% of the samples. Regarding in vivo evaluation of acute systemic toxicity, neither macroscopic abnormalities nor deaths were noted in the animals. Pyrogenicity was not greater than 0.125 UE/ml in any of the samples. The bioburden revealed negative results for microbial growth before sterilization. Regarding the oral irritation potential, in vivo evaluation at 24 and 72 hours showed that the animals had no edema or erythema on the oral mucosa. CONCLUSION: The protocol changes established by the authors to prepare lyophilized bovine cancellous bone at a semi-industrial scale is reproducible and yielded a product with excellent biocompatibility. |
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Galia, Carlos RobertoMacedo, Carlos Alberto de SouzaRosito, RicardoMello, Tielle Müller deCamargo, Lourdes Maria Araújo QuaresmaMoreira, Luis Fernando2012-06-27T01:31:57Z20081807-5932http://hdl.handle.net/10183/49785000716537INTRODUCTION: The use of bone grafts in orthopedic, maxillofacial and dental surgery has been growing. Nevertheless, both fresh autografts and frozen allografts have limitations, and therefore, alternative synthetic or natural biomaterials, such as processed and lyophilized bovine bone graft have been developed. OBJECTIVE: To evaluate in vitro and in vivo biocompatibility of lyophilized bovine bone manufactured in a semi-industrial scale, according to a modifical protocol developed by the authors. METHODS: Samples of bovine cancellous bone were processed according to a protocol developed by Kakiuchi et al., and modi¬fied to process samples of bovine cancellous bone. The following trials were performed: in vitro cytotoxicity, in vivo acute systemic toxicity, in vivo oral irritation potential, in vitro pyrogenic reaction, and bioburden. RESULTS: The in vitro evaluation of lyophilized bovine cancellous bone revealed an absence of cytotoxicity in 100% of the samples. Regarding in vivo evaluation of acute systemic toxicity, neither macroscopic abnormalities nor deaths were noted in the animals. Pyrogenicity was not greater than 0.125 UE/ml in any of the samples. The bioburden revealed negative results for microbial growth before sterilization. Regarding the oral irritation potential, in vivo evaluation at 24 and 72 hours showed that the animals had no edema or erythema on the oral mucosa. CONCLUSION: The protocol changes established by the authors to prepare lyophilized bovine cancellous bone at a semi-industrial scale is reproducible and yielded a product with excellent biocompatibility.application/pdfengClinics. São Paulo. Vol. 63, n. 6 (2008), p. 801-806CirurgiaTestes de toxicidadeLiofilizaçãoTransplante ósseoSurgeryMaterials testingToxicity testsFreeze dryingBone transplantationIn vitro and in vivo evaluation of lyophilized bovine bone biocompatibilityinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000716537.pdf000716537.pdfTexto completo (inglês)application/pdf109559http://www.lume.ufrgs.br/bitstream/10183/49785/1/000716537.pdfdf68fc6a6fe3f81643712e2c5b98265dMD51TEXT000716537.pdf.txt000716537.pdf.txtExtracted Texttext/plain28710http://www.lume.ufrgs.br/bitstream/10183/49785/2/000716537.pdf.txtb8811b64472e25c6292fbd521c58e34eMD52THUMBNAIL000716537.pdf.jpg000716537.pdf.jpgGenerated Thumbnailimage/jpeg1834http://www.lume.ufrgs.br/bitstream/10183/49785/3/000716537.pdf.jpgab1e64829b45a3cce3ad50a1b109a87bMD5310183/497852018-10-29 09:26:26.464oai:www.lume.ufrgs.br:10183/49785Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-29T12:26:26Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility |
title |
In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility |
spellingShingle |
In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility Galia, Carlos Roberto Cirurgia Testes de toxicidade Liofilização Transplante ósseo Surgery Materials testing Toxicity tests Freeze drying Bone transplantation |
title_short |
In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility |
title_full |
In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility |
title_fullStr |
In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility |
title_full_unstemmed |
In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility |
title_sort |
In vitro and in vivo evaluation of lyophilized bovine bone biocompatibility |
author |
Galia, Carlos Roberto |
author_facet |
Galia, Carlos Roberto Macedo, Carlos Alberto de Souza Rosito, Ricardo Mello, Tielle Müller de Camargo, Lourdes Maria Araújo Quaresma Moreira, Luis Fernando |
author_role |
author |
author2 |
Macedo, Carlos Alberto de Souza Rosito, Ricardo Mello, Tielle Müller de Camargo, Lourdes Maria Araújo Quaresma Moreira, Luis Fernando |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Galia, Carlos Roberto Macedo, Carlos Alberto de Souza Rosito, Ricardo Mello, Tielle Müller de Camargo, Lourdes Maria Araújo Quaresma Moreira, Luis Fernando |
dc.subject.por.fl_str_mv |
Cirurgia Testes de toxicidade Liofilização Transplante ósseo |
topic |
Cirurgia Testes de toxicidade Liofilização Transplante ósseo Surgery Materials testing Toxicity tests Freeze drying Bone transplantation |
dc.subject.eng.fl_str_mv |
Surgery Materials testing Toxicity tests Freeze drying Bone transplantation |
description |
INTRODUCTION: The use of bone grafts in orthopedic, maxillofacial and dental surgery has been growing. Nevertheless, both fresh autografts and frozen allografts have limitations, and therefore, alternative synthetic or natural biomaterials, such as processed and lyophilized bovine bone graft have been developed. OBJECTIVE: To evaluate in vitro and in vivo biocompatibility of lyophilized bovine bone manufactured in a semi-industrial scale, according to a modifical protocol developed by the authors. METHODS: Samples of bovine cancellous bone were processed according to a protocol developed by Kakiuchi et al., and modi¬fied to process samples of bovine cancellous bone. The following trials were performed: in vitro cytotoxicity, in vivo acute systemic toxicity, in vivo oral irritation potential, in vitro pyrogenic reaction, and bioburden. RESULTS: The in vitro evaluation of lyophilized bovine cancellous bone revealed an absence of cytotoxicity in 100% of the samples. Regarding in vivo evaluation of acute systemic toxicity, neither macroscopic abnormalities nor deaths were noted in the animals. Pyrogenicity was not greater than 0.125 UE/ml in any of the samples. The bioburden revealed negative results for microbial growth before sterilization. Regarding the oral irritation potential, in vivo evaluation at 24 and 72 hours showed that the animals had no edema or erythema on the oral mucosa. CONCLUSION: The protocol changes established by the authors to prepare lyophilized bovine cancellous bone at a semi-industrial scale is reproducible and yielded a product with excellent biocompatibility. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008 |
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2012-06-27T01:31:57Z |
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000716537 |
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dc.language.iso.fl_str_mv |
eng |
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dc.relation.ispartof.pt_BR.fl_str_mv |
Clinics. São Paulo. Vol. 63, n. 6 (2008), p. 801-806 |
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openAccess |
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