The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/21190 |
Resumo: | We describe the behavior of the snail Megalobulimus abbreviatus upon receiving thermal stimuli and the effects of pretreatment with morphine and naloxone on behavior after a thermal stimulus, in order to establish a useful model for nociceptive experiments. Snails submitted to non-functional (22ºC) and non-thermal hot-plate stress (30ºC) only displayed exploratory behavior. However, the animals submitted to a thermal stimulus (50ºC) displayed biphasic avoidance behavior. Latency was measured from the time the animal was placed on the hot plate to the time when the animal lifted the head-foot complex 1 cm from the substrate, indicating aversive thermal behavior. Other animals were pretreated with morphine (5, 10, 20 mg/kg) or naloxone (2.5, 5.0, 7.5 mg/kg) 15 min prior to receiving a thermal stimulus (50ºC; N = 9 in each group). The results (means ± SD) showed an extremely significant difference in response latency between the group treated with 20 mg/kg morphine (63.18 ± 14.47 s) and the other experimental groups (P < 0.001). With 2.5 mg/kg (16.26 ± 3.19 s), 5.0 mg/kg (11.53 ± 1.64 s) and 7.5 mg/kg naloxone (7.38 ± 1.6 s), there was a significant, not dose-dependent decrease in latency compared to the control (33.44 ± 8.53 s) and saline groups (29.1 ± 9.91 s). No statistically significant difference was found between the naloxone-treated groups. With naloxone plus morphine, there was a significant decrease in latency when compared to all other groups (minimum 64% in the saline group and maximum 83.2% decrease in the morphine group). These results provide evidence of the involvement of endogenous opioid peptides in the control of thermal withdrawal behavior in this snail, and reveal a stereotyped and reproducible avoidance behavior for this snail species, which could be studied in other pharmacological and neurophysiological studies. |
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Achaval-Elena, MatildePenha, Marco Aurélio PereiraSwarowsky, AlessandraSoster, Paula Rigon da LuzXavier, Leder LealViola, Giordano GubertZancan, Denise Maria2010-04-24T04:15:39Z20050100-879Xhttp://hdl.handle.net/10183/21190000519397We describe the behavior of the snail Megalobulimus abbreviatus upon receiving thermal stimuli and the effects of pretreatment with morphine and naloxone on behavior after a thermal stimulus, in order to establish a useful model for nociceptive experiments. Snails submitted to non-functional (22ºC) and non-thermal hot-plate stress (30ºC) only displayed exploratory behavior. However, the animals submitted to a thermal stimulus (50ºC) displayed biphasic avoidance behavior. Latency was measured from the time the animal was placed on the hot plate to the time when the animal lifted the head-foot complex 1 cm from the substrate, indicating aversive thermal behavior. Other animals were pretreated with morphine (5, 10, 20 mg/kg) or naloxone (2.5, 5.0, 7.5 mg/kg) 15 min prior to receiving a thermal stimulus (50ºC; N = 9 in each group). The results (means ± SD) showed an extremely significant difference in response latency between the group treated with 20 mg/kg morphine (63.18 ± 14.47 s) and the other experimental groups (P < 0.001). With 2.5 mg/kg (16.26 ± 3.19 s), 5.0 mg/kg (11.53 ± 1.64 s) and 7.5 mg/kg naloxone (7.38 ± 1.6 s), there was a significant, not dose-dependent decrease in latency compared to the control (33.44 ± 8.53 s) and saline groups (29.1 ± 9.91 s). No statistically significant difference was found between the naloxone-treated groups. With naloxone plus morphine, there was a significant decrease in latency when compared to all other groups (minimum 64% in the saline group and maximum 83.2% decrease in the morphine group). These results provide evidence of the involvement of endogenous opioid peptides in the control of thermal withdrawal behavior in this snail, and reveal a stereotyped and reproducible avoidance behavior for this snail species, which could be studied in other pharmacological and neurophysiological studies.application/pdfengBrazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 38, no. 1 (Jan. 2005), p. 73-80FisiologiaSnailThermal avoidanceNociceptionMorphineNaloxoneThe terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behaviorinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000519397.pdf000519397.pdfTexto completo (inglês)application/pdf649905http://www.lume.ufrgs.br/bitstream/10183/21190/1/000519397.pdfc10c15ecf2c7980db0b967d32e08b8f5MD51TEXT000519397.pdf.txt000519397.pdf.txtExtracted Texttext/plain29043http://www.lume.ufrgs.br/bitstream/10183/21190/2/000519397.pdf.txt29595f26b486ae210c7c2e2d9d7fb1ceMD52THUMBNAIL000519397.pdf.jpg000519397.pdf.jpgGenerated Thumbnailimage/jpeg1819http://www.lume.ufrgs.br/bitstream/10183/21190/3/000519397.pdf.jpgd57cbd1a457d878a3cf524882988c088MD5310183/211902021-11-20 05:42:49.267264oai:www.lume.ufrgs.br:10183/21190Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2021-11-20T07:42:49Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior |
title |
The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior |
spellingShingle |
The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior Achaval-Elena, Matilde Fisiologia Snail Thermal avoidance Nociception Morphine Naloxone |
title_short |
The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior |
title_full |
The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior |
title_fullStr |
The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior |
title_full_unstemmed |
The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior |
title_sort |
The terrestrial Gastropoda megalobulimus abbreviatus as a useful model for nociceptive experiments : effects of morphine and naloxone on thermal avoidance behavior |
author |
Achaval-Elena, Matilde |
author_facet |
Achaval-Elena, Matilde Penha, Marco Aurélio Pereira Swarowsky, Alessandra Soster, Paula Rigon da Luz Xavier, Leder Leal Viola, Giordano Gubert Zancan, Denise Maria |
author_role |
author |
author2 |
Penha, Marco Aurélio Pereira Swarowsky, Alessandra Soster, Paula Rigon da Luz Xavier, Leder Leal Viola, Giordano Gubert Zancan, Denise Maria |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Achaval-Elena, Matilde Penha, Marco Aurélio Pereira Swarowsky, Alessandra Soster, Paula Rigon da Luz Xavier, Leder Leal Viola, Giordano Gubert Zancan, Denise Maria |
dc.subject.por.fl_str_mv |
Fisiologia |
topic |
Fisiologia Snail Thermal avoidance Nociception Morphine Naloxone |
dc.subject.eng.fl_str_mv |
Snail Thermal avoidance Nociception Morphine Naloxone |
description |
We describe the behavior of the snail Megalobulimus abbreviatus upon receiving thermal stimuli and the effects of pretreatment with morphine and naloxone on behavior after a thermal stimulus, in order to establish a useful model for nociceptive experiments. Snails submitted to non-functional (22ºC) and non-thermal hot-plate stress (30ºC) only displayed exploratory behavior. However, the animals submitted to a thermal stimulus (50ºC) displayed biphasic avoidance behavior. Latency was measured from the time the animal was placed on the hot plate to the time when the animal lifted the head-foot complex 1 cm from the substrate, indicating aversive thermal behavior. Other animals were pretreated with morphine (5, 10, 20 mg/kg) or naloxone (2.5, 5.0, 7.5 mg/kg) 15 min prior to receiving a thermal stimulus (50ºC; N = 9 in each group). The results (means ± SD) showed an extremely significant difference in response latency between the group treated with 20 mg/kg morphine (63.18 ± 14.47 s) and the other experimental groups (P < 0.001). With 2.5 mg/kg (16.26 ± 3.19 s), 5.0 mg/kg (11.53 ± 1.64 s) and 7.5 mg/kg naloxone (7.38 ± 1.6 s), there was a significant, not dose-dependent decrease in latency compared to the control (33.44 ± 8.53 s) and saline groups (29.1 ± 9.91 s). No statistically significant difference was found between the naloxone-treated groups. With naloxone plus morphine, there was a significant decrease in latency when compared to all other groups (minimum 64% in the saline group and maximum 83.2% decrease in the morphine group). These results provide evidence of the involvement of endogenous opioid peptides in the control of thermal withdrawal behavior in this snail, and reveal a stereotyped and reproducible avoidance behavior for this snail species, which could be studied in other pharmacological and neurophysiological studies. |
publishDate |
2005 |
dc.date.issued.fl_str_mv |
2005 |
dc.date.accessioned.fl_str_mv |
2010-04-24T04:15:39Z |
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info:eu-repo/semantics/article info:eu-repo/semantics/other |
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article |
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dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/21190 |
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0100-879X |
dc.identifier.nrb.pt_BR.fl_str_mv |
000519397 |
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http://hdl.handle.net/10183/21190 |
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eng |
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eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of medical and biological research = Revista brasileira de pesquisas médicas e biológicas. Ribeirão Preto, SP. Vol. 38, no. 1 (Jan. 2005), p. 73-80 |
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openAccess |
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