Feeding regimen modulates zebrafish behavior
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/197544 |
Resumo: | Here we show that the feeding regimen modulates zebrafish (Danio rerio) behavior. With regard to the time elapsed between feeding and behavioral evaluation, fish fed 3 h before behavioral evaluation in the novel tank test (NTT) showed decreased activity and a trend toward an anxiolytic reaction (increased use of the upper section of the aquarium) in comparison to fish fed 0.5, 6, 12, 24 or 48 h before testing, although differences were not statistically significant for all comparisons. Activity and use of the upper section of the aquarium did not differ significantly among the other treatments. Regarding feeding frequency, fish fed once a day showed higher anxiety-like behavior (decreased use of the upper section of the aquarium) in comparison to fish fed twice a day, but feeding four or six times per day or only every second day did not result in differences from feeding twice a day. Feeding frequency had no effect on activity level. Metabolically, fish fed once a day presented decreased levels of glucose and glycogen and increased lactate when compared to the regular feeding (fish fed twice a day), suggesting that feeding regimen may modulate carbohydrate metabolism. Mechanistically, we suggest that the metabolic changes caused by the feeding regimen may induce behavioral changes. Our results suggest that the high variability of the results among different laboratories might be related to different feeding protocols. Therefore, if issues pertaining to the feeding regimen are not considered during experiments with zebrafish, erroneous interpretations of datasets may occur. |
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Dametto, Fernanda da SilveiraFior, DéboraIdalencio, RenanRosa, João Gabriel SantosFagundes, MicheleMarqueze, AlessandraBarreto, Rodrigo E.Piato, Angelo Luis StapassoliBarcellos, Leonardo Jose Gil2019-07-31T02:29:43Z20182167-8359http://hdl.handle.net/10183/197544001093271Here we show that the feeding regimen modulates zebrafish (Danio rerio) behavior. With regard to the time elapsed between feeding and behavioral evaluation, fish fed 3 h before behavioral evaluation in the novel tank test (NTT) showed decreased activity and a trend toward an anxiolytic reaction (increased use of the upper section of the aquarium) in comparison to fish fed 0.5, 6, 12, 24 or 48 h before testing, although differences were not statistically significant for all comparisons. Activity and use of the upper section of the aquarium did not differ significantly among the other treatments. Regarding feeding frequency, fish fed once a day showed higher anxiety-like behavior (decreased use of the upper section of the aquarium) in comparison to fish fed twice a day, but feeding four or six times per day or only every second day did not result in differences from feeding twice a day. Feeding frequency had no effect on activity level. Metabolically, fish fed once a day presented decreased levels of glucose and glycogen and increased lactate when compared to the regular feeding (fish fed twice a day), suggesting that feeding regimen may modulate carbohydrate metabolism. Mechanistically, we suggest that the metabolic changes caused by the feeding regimen may induce behavioral changes. Our results suggest that the high variability of the results among different laboratories might be related to different feeding protocols. Therefore, if issues pertaining to the feeding regimen are not considered during experiments with zebrafish, erroneous interpretations of datasets may occur.application/pdfengPeerJ. Corte Madera. Vol. 6 (2018), e5343, 17 p.Peixe-zebraComportamentoAlimentaçãoAnxietyNovel tank testDanio rerioEnergetic metabolismFeeding frequencyFeeding regimen modulates zebrafish behaviorEstrangeiroinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSTEXT001093271.pdf.txt001093271.pdf.txtExtracted Texttext/plain47047http://www.lume.ufrgs.br/bitstream/10183/197544/2/001093271.pdf.txt91ff094467de36ed2e68f25f1fd3660dMD52ORIGINAL001093271.pdfTexto completo (inglês)application/pdf1249149http://www.lume.ufrgs.br/bitstream/10183/197544/1/001093271.pdfe0851f81174147cee34948ea30c2dfebMD5110183/1975442019-08-01 02:30:11.804077oai:www.lume.ufrgs.br:10183/197544Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2019-08-01T05:30:11Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Feeding regimen modulates zebrafish behavior |
title |
Feeding regimen modulates zebrafish behavior |
spellingShingle |
Feeding regimen modulates zebrafish behavior Dametto, Fernanda da Silveira Peixe-zebra Comportamento Alimentação Anxiety Novel tank test Danio rerio Energetic metabolism Feeding frequency |
title_short |
Feeding regimen modulates zebrafish behavior |
title_full |
Feeding regimen modulates zebrafish behavior |
title_fullStr |
Feeding regimen modulates zebrafish behavior |
title_full_unstemmed |
Feeding regimen modulates zebrafish behavior |
title_sort |
Feeding regimen modulates zebrafish behavior |
author |
Dametto, Fernanda da Silveira |
author_facet |
Dametto, Fernanda da Silveira Fior, Débora Idalencio, Renan Rosa, João Gabriel Santos Fagundes, Michele Marqueze, Alessandra Barreto, Rodrigo E. Piato, Angelo Luis Stapassoli Barcellos, Leonardo Jose Gil |
author_role |
author |
author2 |
Fior, Débora Idalencio, Renan Rosa, João Gabriel Santos Fagundes, Michele Marqueze, Alessandra Barreto, Rodrigo E. Piato, Angelo Luis Stapassoli Barcellos, Leonardo Jose Gil |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Dametto, Fernanda da Silveira Fior, Débora Idalencio, Renan Rosa, João Gabriel Santos Fagundes, Michele Marqueze, Alessandra Barreto, Rodrigo E. Piato, Angelo Luis Stapassoli Barcellos, Leonardo Jose Gil |
dc.subject.por.fl_str_mv |
Peixe-zebra Comportamento Alimentação |
topic |
Peixe-zebra Comportamento Alimentação Anxiety Novel tank test Danio rerio Energetic metabolism Feeding frequency |
dc.subject.eng.fl_str_mv |
Anxiety Novel tank test Danio rerio Energetic metabolism Feeding frequency |
description |
Here we show that the feeding regimen modulates zebrafish (Danio rerio) behavior. With regard to the time elapsed between feeding and behavioral evaluation, fish fed 3 h before behavioral evaluation in the novel tank test (NTT) showed decreased activity and a trend toward an anxiolytic reaction (increased use of the upper section of the aquarium) in comparison to fish fed 0.5, 6, 12, 24 or 48 h before testing, although differences were not statistically significant for all comparisons. Activity and use of the upper section of the aquarium did not differ significantly among the other treatments. Regarding feeding frequency, fish fed once a day showed higher anxiety-like behavior (decreased use of the upper section of the aquarium) in comparison to fish fed twice a day, but feeding four or six times per day or only every second day did not result in differences from feeding twice a day. Feeding frequency had no effect on activity level. Metabolically, fish fed once a day presented decreased levels of glucose and glycogen and increased lactate when compared to the regular feeding (fish fed twice a day), suggesting that feeding regimen may modulate carbohydrate metabolism. Mechanistically, we suggest that the metabolic changes caused by the feeding regimen may induce behavioral changes. Our results suggest that the high variability of the results among different laboratories might be related to different feeding protocols. Therefore, if issues pertaining to the feeding regimen are not considered during experiments with zebrafish, erroneous interpretations of datasets may occur. |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018 |
dc.date.accessioned.fl_str_mv |
2019-07-31T02:29:43Z |
dc.type.driver.fl_str_mv |
Estrangeiro info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/197544 |
dc.identifier.issn.pt_BR.fl_str_mv |
2167-8359 |
dc.identifier.nrb.pt_BR.fl_str_mv |
001093271 |
identifier_str_mv |
2167-8359 001093271 |
url |
http://hdl.handle.net/10183/197544 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.ispartof.pt_BR.fl_str_mv |
PeerJ. Corte Madera. Vol. 6 (2018), e5343, 17 p. |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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reponame:Repositório Institucional da UFRGS instname:Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
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UFRGS |
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Repositório Institucional da UFRGS |
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Repositório Institucional da UFRGS |
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