Progressive disseminated histoplasmosis : a systematic review on the performance of non-culture-based diagnostic tests

• Autor(a) principal: Falci, Diego Rodrigues
• Data de Publicação: 2017
• Outros Autores: Hoffmann, Elias da Rosa, Paskulin, Diego D'Ávila, Pasqualotto, Alessandro Comaru
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRGS
• Texto Completo: http://hdl.handle.net/10183/185282
• topic: Histoplasmose; Diagnóstico; Técnicas imunoenzimáticas; Reação em cadeia da polimerase; Métodos; Sensibilidade e especificidade; Testes sorológicos; Disseminated histoplasmosis; Non-culture; Enzyme immunoassay; Diagnostic testsa

The diagnosis of progressive disseminated histoplasmosis is often a challenge to clinicians,especially due to the low sensitivity and long turnaround time of the classic diagnostic meth-ods. In recent years, studies involving a variety of non-culture-based diagnostic tests havebeen published in the literature. We performed a systematic review by selecting studies eval-uating non-culture-based diagnostic methods for progressive disseminated histoplasmosis.We searched for articles evaluating detection of antibody, antigens, as well as DNA-baseddiagnostic methods. A comprehensive PUBMED, Web of Science, and Cochrane Librarysearch was performed between the years 1956 and 2016. Case reports, review articles, non-human models and series involving less than 10 patients were excluded. We found 278articles and after initial review 18 articles were included: (12) involved antigen detectionmethods, (4) molecular methods, and (2) antibody detection methods. Here we demonstratethat the pursuit of new technologies is ultimately required for the early and accurate diag-nosis of disseminated histoplasmosis. In particular, urinary antigen detection was the mostaccurate tool when compared with other diagnostic techniques.