Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Floresta e Ambiente |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872020000200111 |
Resumo: | Abstract Pecan cultivation in Brazil has gained increasingly economic importance in the southern part of the country. The objective of this work was to identify the pathogen that causes root rot in Carya illinoinensis seedlings in the nursery, through morphological, molecular and pathogenicity tests. Fungi from the genus Fusarium found in symptomatic roots of pecan seedlings were isolated in potato dextrose agar (PDA) culture medium and purified. Morphological characterization was performed in PDA and carnation leaf agar (CLA) media. For the molecular characterization, the genomic segments ITS (internal transcribed spaces), β-tub (beta-tubulin), and tef1-α (elongation fact 1-alpha) were sequenced. The pathogenicity test was performed on healthy seedlings in the greenhouse. The combination of morphological and molecular characters was fundamental for identification of the species, showing that the most suitable genes for identification were ITS and tef1-α. The causative agent of root rot in C. illinoinensis seedlings in the nursery was identified as Fusarium oxysporum. |
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Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlingsetiologyroot rotpecanAbstract Pecan cultivation in Brazil has gained increasingly economic importance in the southern part of the country. The objective of this work was to identify the pathogen that causes root rot in Carya illinoinensis seedlings in the nursery, through morphological, molecular and pathogenicity tests. Fungi from the genus Fusarium found in symptomatic roots of pecan seedlings were isolated in potato dextrose agar (PDA) culture medium and purified. Morphological characterization was performed in PDA and carnation leaf agar (CLA) media. For the molecular characterization, the genomic segments ITS (internal transcribed spaces), β-tub (beta-tubulin), and tef1-α (elongation fact 1-alpha) were sequenced. The pathogenicity test was performed on healthy seedlings in the greenhouse. The combination of morphological and molecular characters was fundamental for identification of the species, showing that the most suitable genes for identification were ITS and tef1-α. The causative agent of root rot in C. illinoinensis seedlings in the nursery was identified as Fusarium oxysporum.Instituto de Florestas da Universidade Federal Rural do Rio de Janeiro2020-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872020000200111Floresta e Ambiente v.27 n.2 2020reponame:Floresta e Ambienteinstname:Universidade Federal do Rio de Janeiro (UFRJ)instacron:UFRJ10.1590/2179-8087.108917info:eu-repo/semantics/openAccessPoletto,TalesMuniz,Marlove Fátima BriãoFantinel,Vinícius SpolaorHarakava,RicardoRolim,Jéssica Mengueeng2020-08-05T00:00:00Zoai:scielo:S2179-80872020000200111Revistahttps://www.floram.org/PUBhttps://old.scielo.br/oai/scielo-oai.phpfloramjournal@gmail.com||floram@ufrrj.br||2179-80871415-0980opendoar:2020-08-05T00:00Floresta e Ambiente - Universidade Federal do Rio de Janeiro (UFRJ)false |
dc.title.none.fl_str_mv |
Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
title |
Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
spellingShingle |
Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings Poletto,Tales etiology root rot pecan |
title_short |
Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
title_full |
Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
title_fullStr |
Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
title_full_unstemmed |
Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
title_sort |
Characterization and Pathogenicity of Fusarium oxysporum Associated with Carya illinoinensis Seedlings |
author |
Poletto,Tales |
author_facet |
Poletto,Tales Muniz,Marlove Fátima Brião Fantinel,Vinícius Spolaor Harakava,Ricardo Rolim,Jéssica Mengue |
author_role |
author |
author2 |
Muniz,Marlove Fátima Brião Fantinel,Vinícius Spolaor Harakava,Ricardo Rolim,Jéssica Mengue |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Poletto,Tales Muniz,Marlove Fátima Brião Fantinel,Vinícius Spolaor Harakava,Ricardo Rolim,Jéssica Mengue |
dc.subject.por.fl_str_mv |
etiology root rot pecan |
topic |
etiology root rot pecan |
description |
Abstract Pecan cultivation in Brazil has gained increasingly economic importance in the southern part of the country. The objective of this work was to identify the pathogen that causes root rot in Carya illinoinensis seedlings in the nursery, through morphological, molecular and pathogenicity tests. Fungi from the genus Fusarium found in symptomatic roots of pecan seedlings were isolated in potato dextrose agar (PDA) culture medium and purified. Morphological characterization was performed in PDA and carnation leaf agar (CLA) media. For the molecular characterization, the genomic segments ITS (internal transcribed spaces), β-tub (beta-tubulin), and tef1-α (elongation fact 1-alpha) were sequenced. The pathogenicity test was performed on healthy seedlings in the greenhouse. The combination of morphological and molecular characters was fundamental for identification of the species, showing that the most suitable genes for identification were ITS and tef1-α. The causative agent of root rot in C. illinoinensis seedlings in the nursery was identified as Fusarium oxysporum. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872020000200111 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S2179-80872020000200111 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/2179-8087.108917 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto de Florestas da Universidade Federal Rural do Rio de Janeiro |
publisher.none.fl_str_mv |
Instituto de Florestas da Universidade Federal Rural do Rio de Janeiro |
dc.source.none.fl_str_mv |
Floresta e Ambiente v.27 n.2 2020 reponame:Floresta e Ambiente instname:Universidade Federal do Rio de Janeiro (UFRJ) instacron:UFRJ |
instname_str |
Universidade Federal do Rio de Janeiro (UFRJ) |
instacron_str |
UFRJ |
institution |
UFRJ |
reponame_str |
Floresta e Ambiente |
collection |
Floresta e Ambiente |
repository.name.fl_str_mv |
Floresta e Ambiente - Universidade Federal do Rio de Janeiro (UFRJ) |
repository.mail.fl_str_mv |
floramjournal@gmail.com||floram@ufrrj.br|| |
_version_ |
1750128143311568896 |