Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings

Detalhes bibliográficos
Autor(a) principal: Maciel, Caciara Gonzatto
Data de Publicação: 2022
Outros Autores: Silveira, Isabele Simões, Lazarotto, Marília, Fior, Claudimar Sidnei, Hilgert, Marcio Alberto
Tipo de documento: Artigo
Idioma: por
Título da fonte: Ciência Florestal (Online)
Texto Completo: https://periodicos.ufsm.br/cienciaflorestal/article/view/67140
Resumo: Pecan seedlings production has increased in recent decades, especially in southern Brazil, consequently, intensifies studies in crop phytosanitary problems in this region. Sanitary quality is an important factor in the production of forest seedlings, considering the occurrence of diseases in all stages of the production process. In April 2021, dark and irregular leaf spots were observed in pecan seedlings under nursery conditions. The evolution of symptoms caused leaf drop and compromised plant development, due to the reduction of their photosynthetic area. It was verified that it was a fungus, which was isolated on potato-dextrose-agar (PDA) medium, and later had its pathogenicity verified in detached pecan leaflets. Four-mm disks of the pure culture of the Phomopsis sp. were placed on the surface of healthy leaflets, previously injuredwith a sterilized histological needle. After inoculation, the leaflets were placed in gerbox boxes lined with two sheets of sterilized filter paper and moistened with sterilized distilled water and kept in an incubation chamber for seven days at 25 ± 2ºC and 12 h photoperiod. Eight healthy leaflets were used as controls, which were subjected to the same conditions, except for fungus inoculation, using only disks of PDA culture medium. The isolate proved to be pathogenic, with the first symptoms after 6 days of inoculation, the initially circular and necrotic lesions coalesced and at 10 days reached 16 mm. The re-isolation was carried out as soon as the reproductive structures of the fungus appeared, these were transferred to Petri dishes with PDA culture medium. The fungus causing the symptoms was identified as Phomopsis sp. and, this work presents the first report of the occurrence of Phomopsis sp. in Carya illinoinensis under nursery conditions in Brazil.
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spelling Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlingsOcorrência de Phomopsis sp. em mudas de Carya illinoinensis (Wangenh) K. KochPatogenicidadeNogueira-pecanFungoMancha foliarPathogenicityPecanFungusLeaf spotPecan seedlings production has increased in recent decades, especially in southern Brazil, consequently, intensifies studies in crop phytosanitary problems in this region. Sanitary quality is an important factor in the production of forest seedlings, considering the occurrence of diseases in all stages of the production process. In April 2021, dark and irregular leaf spots were observed in pecan seedlings under nursery conditions. The evolution of symptoms caused leaf drop and compromised plant development, due to the reduction of their photosynthetic area. It was verified that it was a fungus, which was isolated on potato-dextrose-agar (PDA) medium, and later had its pathogenicity verified in detached pecan leaflets. Four-mm disks of the pure culture of the Phomopsis sp. were placed on the surface of healthy leaflets, previously injuredwith a sterilized histological needle. After inoculation, the leaflets were placed in gerbox boxes lined with two sheets of sterilized filter paper and moistened with sterilized distilled water and kept in an incubation chamber for seven days at 25 ± 2ºC and 12 h photoperiod. Eight healthy leaflets were used as controls, which were subjected to the same conditions, except for fungus inoculation, using only disks of PDA culture medium. The isolate proved to be pathogenic, with the first symptoms after 6 days of inoculation, the initially circular and necrotic lesions coalesced and at 10 days reached 16 mm. The re-isolation was carried out as soon as the reproductive structures of the fungus appeared, these were transferred to Petri dishes with PDA culture medium. The fungus causing the symptoms was identified as Phomopsis sp. and, this work presents the first report of the occurrence of Phomopsis sp. in Carya illinoinensis under nursery conditions in Brazil.A produção de mudas de nogueira-pecan tem ganhado força nas últimas décadas, principalmente na região sul do Brasil, e esse aumento na demanda, consequentemente, intensifica os estudos sobre problemas fitossanitários presentes na cultura nessa região. A qualidade sanitária é um fator importante na produção de mudas florestais, tendo em vista a ocorrência de doenças em todas as etapas do processo produtivo. Em abril de 2021, foram observadas manchas foliares escuras e irregulares em mudas de nogueira-pecan em condições de viveiro. A evolução dos sintomas causava queda dos folíolos e comprometimento no desenvolvimento das plantas, devido à redução da sua área fotossintética. Verificou-se que se tratava do efeito causal de um fungo, que foi isolado em meio batata-dextrose-ágar (BDA), tendo, posteriormente, sua patogenicidade constatada em folíolos destacados de nogueira-pecan. Discos de 4 mm da cultura pura do isolado de Phomopsis sp. foram dispostos na superfície de folíolos sadios, previamente feridos com agulha histológica esterilizada. Após a inoculação, os folíolos foram acondicionados em caixas gerbox forradas com duas folhas de papel-filtro esterilizadas e umedecidas com água destilada esterilizada e mantidas em câmara de incubação durante sete dias a 25 ± 2ºC e fotoperíodo de 12 h. Foram utilizados oito folíolos sadios como testemunha, os quais foram submetidos às mesmas condições, utilizando apenas discos de meio de cultura BDA. O isolado mostrou-se patogênico, com os primeiros sintomas após 6 dias da inoculação. As lesões, inicialmente circulares e necróticas, foram coalescendo e aos 10 dias atingiram 16 mm. Foi realizado o reisolamento assim que as estruturas reprodutivas do fungo apareceram, essas foram transferidas para placas de Petri com meio de cultura BDA. O fungo causador dos sintomas foi identificado como Phomopsis sp., e este trabalho apresenta o primeiro relato da ocorrência de mancha foliar de Phomopsis sp. em Carya illinoinensis em condições de viveiro no Brasil.Universidade Federal de Santa Maria2022-06-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmltext/xmlhttps://periodicos.ufsm.br/cienciaflorestal/article/view/6714010.5902/1980509867140Ciência Florestal; Vol. 32 No. 2 (2022); 1095-1105Ciência Florestal; v. 32 n. 2 (2022); 1095-11051980-50980103-9954reponame:Ciência Florestal (Online)instname:Universidade Federal de Santa Maria (UFSM)instacron:UFSMporhttps://periodicos.ufsm.br/cienciaflorestal/article/view/67140/47560https://periodicos.ufsm.br/cienciaflorestal/article/view/67140/48572https://periodicos.ufsm.br/cienciaflorestal/article/view/67140/50793Copyright (c) 2022 Ciência Florestalhttp://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessMaciel, Caciara GonzattoSilveira, Isabele SimõesLazarotto, MaríliaFior, Claudimar SidneiHilgert, Marcio Alberto2022-12-16T18:51:58Zoai:ojs.pkp.sfu.ca:article/67140Revistahttp://www.ufsm.br/cienciaflorestal/ONGhttps://old.scielo.br/oai/scielo-oai.php||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br1980-50980103-9954opendoar:2022-12-16T18:51:58Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)false
dc.title.none.fl_str_mv Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings
Ocorrência de Phomopsis sp. em mudas de Carya illinoinensis (Wangenh) K. Koch
title Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings
spellingShingle Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings
Maciel, Caciara Gonzatto
Patogenicidade
Nogueira-pecan
Fungo
Mancha foliar
Pathogenicity
Pecan
Fungus
Leaf spot
title_short Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings
title_full Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings
title_fullStr Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings
title_full_unstemmed Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings
title_sort Phomopsis sp. ocurrence in Carya illinoinensis (Wangenh) K. Koch seedlings
author Maciel, Caciara Gonzatto
author_facet Maciel, Caciara Gonzatto
Silveira, Isabele Simões
Lazarotto, Marília
Fior, Claudimar Sidnei
Hilgert, Marcio Alberto
author_role author
author2 Silveira, Isabele Simões
Lazarotto, Marília
Fior, Claudimar Sidnei
Hilgert, Marcio Alberto
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Maciel, Caciara Gonzatto
Silveira, Isabele Simões
Lazarotto, Marília
Fior, Claudimar Sidnei
Hilgert, Marcio Alberto
dc.subject.por.fl_str_mv Patogenicidade
Nogueira-pecan
Fungo
Mancha foliar
Pathogenicity
Pecan
Fungus
Leaf spot
topic Patogenicidade
Nogueira-pecan
Fungo
Mancha foliar
Pathogenicity
Pecan
Fungus
Leaf spot
description Pecan seedlings production has increased in recent decades, especially in southern Brazil, consequently, intensifies studies in crop phytosanitary problems in this region. Sanitary quality is an important factor in the production of forest seedlings, considering the occurrence of diseases in all stages of the production process. In April 2021, dark and irregular leaf spots were observed in pecan seedlings under nursery conditions. The evolution of symptoms caused leaf drop and compromised plant development, due to the reduction of their photosynthetic area. It was verified that it was a fungus, which was isolated on potato-dextrose-agar (PDA) medium, and later had its pathogenicity verified in detached pecan leaflets. Four-mm disks of the pure culture of the Phomopsis sp. were placed on the surface of healthy leaflets, previously injuredwith a sterilized histological needle. After inoculation, the leaflets were placed in gerbox boxes lined with two sheets of sterilized filter paper and moistened with sterilized distilled water and kept in an incubation chamber for seven days at 25 ± 2ºC and 12 h photoperiod. Eight healthy leaflets were used as controls, which were subjected to the same conditions, except for fungus inoculation, using only disks of PDA culture medium. The isolate proved to be pathogenic, with the first symptoms after 6 days of inoculation, the initially circular and necrotic lesions coalesced and at 10 days reached 16 mm. The re-isolation was carried out as soon as the reproductive structures of the fungus appeared, these were transferred to Petri dishes with PDA culture medium. The fungus causing the symptoms was identified as Phomopsis sp. and, this work presents the first report of the occurrence of Phomopsis sp. in Carya illinoinensis under nursery conditions in Brazil.
publishDate 2022
dc.date.none.fl_str_mv 2022-06-24
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/67140
10.5902/1980509867140
url https://periodicos.ufsm.br/cienciaflorestal/article/view/67140
identifier_str_mv 10.5902/1980509867140
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://periodicos.ufsm.br/cienciaflorestal/article/view/67140/47560
https://periodicos.ufsm.br/cienciaflorestal/article/view/67140/48572
https://periodicos.ufsm.br/cienciaflorestal/article/view/67140/50793
dc.rights.driver.fl_str_mv Copyright (c) 2022 Ciência Florestal
http://creativecommons.org/licenses/by-nc/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Ciência Florestal
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
text/xml
dc.publisher.none.fl_str_mv Universidade Federal de Santa Maria
publisher.none.fl_str_mv Universidade Federal de Santa Maria
dc.source.none.fl_str_mv Ciência Florestal; Vol. 32 No. 2 (2022); 1095-1105
Ciência Florestal; v. 32 n. 2 (2022); 1095-1105
1980-5098
0103-9954
reponame:Ciência Florestal (Online)
instname:Universidade Federal de Santa Maria (UFSM)
instacron:UFSM
instname_str Universidade Federal de Santa Maria (UFSM)
instacron_str UFSM
institution UFSM
reponame_str Ciência Florestal (Online)
collection Ciência Florestal (Online)
repository.name.fl_str_mv Ciência Florestal (Online) - Universidade Federal de Santa Maria (UFSM)
repository.mail.fl_str_mv ||cienciaflorestal@ufsm.br|| cienciaflorestal@gmail.com|| cf@smail.ufsm.br
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