Avaliação de estratégias de produção do antígeno 503 recombinante de Leishmania i. chagasi expresso em Escherichia coli em biorreator de bancada

• Autor(a) principal: Vasconcelos, Luan Tales Costa de Paiva
• Data de Publicação: 2015
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFRN
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/38760
• topic: Antígeno 503; 503 antigen; Indução; Escherichia coli Recombinante; Leishmania i. chagasi; Induction; Recombinant Escherichia coli; Leishmania chagasi; Engenharias.

After the advent of recombinant DNA technology, biomolecules were able to be produced on an industrial scale. Heterologous products processed by large-scale bioprocesses largely use Escherichia coli as a host organism. Although there are extensive studies in biotechnology and immunology, currently our level of science failed to find any drug with prophylactic effect that promotes the prevention of leishmaniasis. As a consequence, it is necessary to diagnose antigens that are specific for a deeper study of the development of vaccines and tests for recognition this disease. By the above, the paper aims to identify the influences of the quality of inducer (IPTG or lactose) as well as the induction strategies with respect to the time of induction and concentration of inducer molecule, in the cultivation of Escherichia coli for the production of antigen 503 of Leishmania i. chagasi in rotary shaker and benchtop bioreactor. According to the results collected, it can be noted that the lactose as an inducer component favored the production of the heterologous protein, and it may be the more appropriate inducer, due to factors such as cost and cell toxicity. Late induction did not find high productivity and induction with higher concentration did not influence it positively. The induction pulses provided more nutrients to the bacteria and induced the expression of the antigen at different times from the same cultivation, but it can be seen that this was not the best strategy for the optimization of high protein concentrations.