Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/handle/123456789/32112 |
Resumo: | Traumatic injury to the spinal cord results in a rapid and significant loss of function. One barrier to successful regeneration in the adult CNS is the diminished axonal growth capacity after maturation. Therefore, strategies that seek to promote the restoration of function to the chronically injured spinal cord have high therapeutic value. Neurotrophic factors and peripheral nerves are known to be good substrates for bridging the lesions associatedwith CNS trauma. The role of fibroblast growth factor-2, when added to the sciatic nerve, was examined following spinal cord injury in a rat. We evaluated whether FGF-2 added to a sciatic nerve graft placed in a gap promoted nerve recovery following a complete transection of the spinal cord and if it could enhance neuronal plasticity. Rats underwent a transection at the thoracic level, which was repaired with saline or a fragment of the sciatic nerve. In another group, FGF-2 was added immediately after thelesion. The effects of FGF-2 and the fragment of the sciatic nerve graft on neuronal plasticity were investigated at the epicenterof the injury using NF-200 and 5-HT immunoreactivity after 8 weeks.A high number of NF-200 and 5-HT immunoreactive fibers were observed in the treated groups with sciatic nerve graft in the presence or absence of FGF-2 when compared to the saline group. However, a small number of NF-200(p=0.03)and 5-HT fibers were observed in the epicenter of the graft when FGF-2 was added,when compared to the group that received sciatic nerve graft. These results indicate that sciatic nerve grafting favors the growth of fibers in the traumatized spinal cord, an effect that is slightly influenced by the addition of FGF-2 to the NF-200 and 5-HT immunoreactive fibers |
id |
UFRN_0364effc7b2adabf01505e1be251dd68 |
---|---|
oai_identifier_str |
oai:https://repositorio.ufrn.br:123456789/32112 |
network_acronym_str |
UFRN |
network_name_str |
Repositório Institucional da UFRN |
repository_id_str |
|
spelling |
Fernandes, Aline Samaya SantosCastro, Aline Brito Ferreira deRêgo, Amália Cinthia Meneses doAraújo Filho, IramiLucena, Eudes Euler de SouzaCavalcanti, José Rodolfo Lopes de PaivaCosta, Ianara Mendonça daCavalcante, Jeferson de SouzaCosta, Mirian Stela Maris de OliveiraGuzen, Fausto Pierdoná2021-04-08T10:44:55Z2021-04-08T10:44:55Z2018-07FERNANDES, Aline Samaya Santos; CASTRO, Aline Brito Ferreira de; RÊGO, Amália Cinthia Meneses do; ARAÚJO FILHO, Irami; LUCENA, Eudes Euler de Souza; CAVALCANTI, José Rodolfo Lopes de Paiva; COSTA, Ianara Mendonça da; CAVALCANTE, Jeferson de Souza; COSTA, Mirian Stela Maris de Oliveira; GUZEN, Fausto Pierdoná. Combination sciatic nerve graft and fibroblastic growth factor 2 promotes tissue regeneration for NF-200 and 5-HT in spinal cord injury. Research Journal Of Pharmaceutical, Biological And Chemical Sciences, [s. l.], v. 4, n. 9, p. 246-254, jul. 2018. Disponível em: https://www.rjpbcs.com/2018_9.4.html. Acesso em: 20 jul. 2020.0975-8585https://repositorio.ufrn.br/handle/123456789/32112Research Journal of Pharmaceutical, Biological and Chemical SciencesFibroblastic growth factor-2PlasticityRegenerationSciatic nerve graft and spinal cordCombination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injuryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTraumatic injury to the spinal cord results in a rapid and significant loss of function. One barrier to successful regeneration in the adult CNS is the diminished axonal growth capacity after maturation. Therefore, strategies that seek to promote the restoration of function to the chronically injured spinal cord have high therapeutic value. Neurotrophic factors and peripheral nerves are known to be good substrates for bridging the lesions associatedwith CNS trauma. The role of fibroblast growth factor-2, when added to the sciatic nerve, was examined following spinal cord injury in a rat. We evaluated whether FGF-2 added to a sciatic nerve graft placed in a gap promoted nerve recovery following a complete transection of the spinal cord and if it could enhance neuronal plasticity. Rats underwent a transection at the thoracic level, which was repaired with saline or a fragment of the sciatic nerve. In another group, FGF-2 was added immediately after thelesion. The effects of FGF-2 and the fragment of the sciatic nerve graft on neuronal plasticity were investigated at the epicenterof the injury using NF-200 and 5-HT immunoreactivity after 8 weeks.A high number of NF-200 and 5-HT immunoreactive fibers were observed in the treated groups with sciatic nerve graft in the presence or absence of FGF-2 when compared to the saline group. However, a small number of NF-200(p=0.03)and 5-HT fibers were observed in the epicenter of the graft when FGF-2 was added,when compared to the group that received sciatic nerve graft. These results indicate that sciatic nerve grafting favors the growth of fibers in the traumatized spinal cord, an effect that is slightly influenced by the addition of FGF-2 to the NF-200 and 5-HT immunoreactive fibersengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNinfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32112/2/license.txte9597aa2854d128fd968be5edc8a28d9MD52TEXTTissueRegenerationSpinal_Lucena_2018.pdf.txtTissueRegenerationSpinal_Lucena_2018.pdf.txtExtracted texttext/plain30602https://repositorio.ufrn.br/bitstream/123456789/32112/3/TissueRegenerationSpinal_Lucena_2018.pdf.txt16779dbc0e499377496efdb4c3dce8d7MD53THUMBNAILTissueRegenerationSpinal_Lucena_2018.pdf.jpgTissueRegenerationSpinal_Lucena_2018.pdf.jpgGenerated Thumbnailimage/jpeg1627https://repositorio.ufrn.br/bitstream/123456789/32112/4/TissueRegenerationSpinal_Lucena_2018.pdf.jpg83a52461aa92ad7cfd9bf0bfcba2b888MD54123456789/321122022-04-25 19:19:32.18oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2022-04-25T22:19:32Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury |
title |
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury |
spellingShingle |
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury Fernandes, Aline Samaya Santos Fibroblastic growth factor-2 Plasticity Regeneration Sciatic nerve graft and spinal cord |
title_short |
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury |
title_full |
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury |
title_fullStr |
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury |
title_full_unstemmed |
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury |
title_sort |
Combination sciatic nerve graft and fibroblastic growth factor 2 promotestissue regeneration for NF-200 and 5-HT in spinal cord injury |
author |
Fernandes, Aline Samaya Santos |
author_facet |
Fernandes, Aline Samaya Santos Castro, Aline Brito Ferreira de Rêgo, Amália Cinthia Meneses do Araújo Filho, Irami Lucena, Eudes Euler de Souza Cavalcanti, José Rodolfo Lopes de Paiva Costa, Ianara Mendonça da Cavalcante, Jeferson de Souza Costa, Mirian Stela Maris de Oliveira Guzen, Fausto Pierdoná |
author_role |
author |
author2 |
Castro, Aline Brito Ferreira de Rêgo, Amália Cinthia Meneses do Araújo Filho, Irami Lucena, Eudes Euler de Souza Cavalcanti, José Rodolfo Lopes de Paiva Costa, Ianara Mendonça da Cavalcante, Jeferson de Souza Costa, Mirian Stela Maris de Oliveira Guzen, Fausto Pierdoná |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Fernandes, Aline Samaya Santos Castro, Aline Brito Ferreira de Rêgo, Amália Cinthia Meneses do Araújo Filho, Irami Lucena, Eudes Euler de Souza Cavalcanti, José Rodolfo Lopes de Paiva Costa, Ianara Mendonça da Cavalcante, Jeferson de Souza Costa, Mirian Stela Maris de Oliveira Guzen, Fausto Pierdoná |
dc.subject.por.fl_str_mv |
Fibroblastic growth factor-2 Plasticity Regeneration Sciatic nerve graft and spinal cord |
topic |
Fibroblastic growth factor-2 Plasticity Regeneration Sciatic nerve graft and spinal cord |
description |
Traumatic injury to the spinal cord results in a rapid and significant loss of function. One barrier to successful regeneration in the adult CNS is the diminished axonal growth capacity after maturation. Therefore, strategies that seek to promote the restoration of function to the chronically injured spinal cord have high therapeutic value. Neurotrophic factors and peripheral nerves are known to be good substrates for bridging the lesions associatedwith CNS trauma. The role of fibroblast growth factor-2, when added to the sciatic nerve, was examined following spinal cord injury in a rat. We evaluated whether FGF-2 added to a sciatic nerve graft placed in a gap promoted nerve recovery following a complete transection of the spinal cord and if it could enhance neuronal plasticity. Rats underwent a transection at the thoracic level, which was repaired with saline or a fragment of the sciatic nerve. In another group, FGF-2 was added immediately after thelesion. The effects of FGF-2 and the fragment of the sciatic nerve graft on neuronal plasticity were investigated at the epicenterof the injury using NF-200 and 5-HT immunoreactivity after 8 weeks.A high number of NF-200 and 5-HT immunoreactive fibers were observed in the treated groups with sciatic nerve graft in the presence or absence of FGF-2 when compared to the saline group. However, a small number of NF-200(p=0.03)and 5-HT fibers were observed in the epicenter of the graft when FGF-2 was added,when compared to the group that received sciatic nerve graft. These results indicate that sciatic nerve grafting favors the growth of fibers in the traumatized spinal cord, an effect that is slightly influenced by the addition of FGF-2 to the NF-200 and 5-HT immunoreactive fibers |
publishDate |
2018 |
dc.date.issued.fl_str_mv |
2018-07 |
dc.date.accessioned.fl_str_mv |
2021-04-08T10:44:55Z |
dc.date.available.fl_str_mv |
2021-04-08T10:44:55Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FERNANDES, Aline Samaya Santos; CASTRO, Aline Brito Ferreira de; RÊGO, Amália Cinthia Meneses do; ARAÚJO FILHO, Irami; LUCENA, Eudes Euler de Souza; CAVALCANTI, José Rodolfo Lopes de Paiva; COSTA, Ianara Mendonça da; CAVALCANTE, Jeferson de Souza; COSTA, Mirian Stela Maris de Oliveira; GUZEN, Fausto Pierdoná. Combination sciatic nerve graft and fibroblastic growth factor 2 promotes tissue regeneration for NF-200 and 5-HT in spinal cord injury. Research Journal Of Pharmaceutical, Biological And Chemical Sciences, [s. l.], v. 4, n. 9, p. 246-254, jul. 2018. Disponível em: https://www.rjpbcs.com/2018_9.4.html. Acesso em: 20 jul. 2020. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/handle/123456789/32112 |
dc.identifier.issn.none.fl_str_mv |
0975-8585 |
identifier_str_mv |
FERNANDES, Aline Samaya Santos; CASTRO, Aline Brito Ferreira de; RÊGO, Amália Cinthia Meneses do; ARAÚJO FILHO, Irami; LUCENA, Eudes Euler de Souza; CAVALCANTI, José Rodolfo Lopes de Paiva; COSTA, Ianara Mendonça da; CAVALCANTE, Jeferson de Souza; COSTA, Mirian Stela Maris de Oliveira; GUZEN, Fausto Pierdoná. Combination sciatic nerve graft and fibroblastic growth factor 2 promotes tissue regeneration for NF-200 and 5-HT in spinal cord injury. Research Journal Of Pharmaceutical, Biological And Chemical Sciences, [s. l.], v. 4, n. 9, p. 246-254, jul. 2018. Disponível em: https://www.rjpbcs.com/2018_9.4.html. Acesso em: 20 jul. 2020. 0975-8585 |
url |
https://repositorio.ufrn.br/handle/123456789/32112 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Research Journal of Pharmaceutical, Biological and Chemical Sciences |
publisher.none.fl_str_mv |
Research Journal of Pharmaceutical, Biological and Chemical Sciences |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
instname_str |
Universidade Federal do Rio Grande do Norte (UFRN) |
instacron_str |
UFRN |
institution |
UFRN |
reponame_str |
Repositório Institucional da UFRN |
collection |
Repositório Institucional da UFRN |
bitstream.url.fl_str_mv |
https://repositorio.ufrn.br/bitstream/123456789/32112/2/license.txt https://repositorio.ufrn.br/bitstream/123456789/32112/3/TissueRegenerationSpinal_Lucena_2018.pdf.txt https://repositorio.ufrn.br/bitstream/123456789/32112/4/TissueRegenerationSpinal_Lucena_2018.pdf.jpg |
bitstream.checksum.fl_str_mv |
e9597aa2854d128fd968be5edc8a28d9 16779dbc0e499377496efdb4c3dce8d7 83a52461aa92ad7cfd9bf0bfcba2b888 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN) |
repository.mail.fl_str_mv |
|
_version_ |
1802117865229254656 |