Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice

Detalhes bibliográficos
Autor(a) principal: Borges, Thawann Malfatti
Data de Publicação: 2015
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/jspui/handle/123456789/20343
Resumo: Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
id UFRN_03f996594efdae2d71bf606c2959523e
oai_identifier_str oai:https://repositorio.ufrn.br:123456789/20343
network_acronym_str UFRN
network_name_str Repositório Institucional da UFRN
repository_id_str
spelling Borges, Thawann Malfattihttp://lattes.cnpq.br/1262934391596208http://lattes.cnpq.br/1279823352935722Leão, Ricardo Maurício Xavierhttp://lattes.cnpq.br/8125613125649746Leão, Richardson Naveshttp://lattes.cnpq.br/0683942077872227Leão, Emelie Katarina Svahn2016-04-29T20:35:41Z2016-04-29T20:35:41Z2015-06-26BORGES, Thawann Malfatti. Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice. 2015. 60f. Dissertação (Mestrado em Neurociências) - Universidade Federal do Rio Grande do Norte, Natal, 2015.https://repositorio.ufrn.br/jspui/handle/123456789/20343Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Tinnitus is the perception of a sound in the absence of a corresponding physical stimulus. It is not clear yet what mechanisms are involved in tinnitus and how it starts and/or becomes chronic. Due to the relationship between tinnitus and somatosensory trauma/stimuli, the dorsal cochlear nucleus (DCN), a region known to integrate somatosensory and auditory pathways, has been identified as a potential key structure in the generation of phantom sound perception. Here, we target specific neuronal populations in the DCN to allow further investigation on how this region may contribute to the generation of tinnitus signals that spread to other auditory areas. We examined the expression of optogenetic proteins (Channelrhodopsin 2 - ChR2; and enhanced Archaerhodopsin 3.0 - eArch3.0), targeting neurons expressing Calmoduline Kinase II alpha (CaMKIIa) promoter in wild-type C57/Bl6 mice and neurons expressing nicotinic acetylcholine receptor subunit alpha-2 promoter (ChRNA2) in ChRNA2- Cre transgenic C57/Bl6 mice, using local virus injection, verified by fluorescence microscopy. Unit responses were differentiated based on their electrophysiological response to sound. We then investigated if firing of neurons expressing optogenetic tools can be controlled in vivo and if the same neurons also fire action potentials in response to precisely timed sound stimulation. Both in vivo and preliminary in vitro data shows that neurons expressing ChR2 do respond to sound, and that they furthermore also can respond to light stimulation with a stable and similar waveform. Moreover, in vivo data shows that neurons expressing eArch3.0, responding to sound, will decrease their firing rate when exposed to green light. Thereby showing that optogenetic tools can be used functionally in the DCN, it is possible to further test tinnitus theories by, for example, producing an increased firing rate in the DCN, trying to mimic tinnitus; or inhibiting increased spontaneous firing rate in the DCN of animals with noise-induced or salycilate-induced tinnitus.porUniversidade Federal do Rio Grande do NortePROGRAMA DE PÓS-GRADUAÇÃO EM NEUROCIÊNCIASUFRNBrasilCNPQ::OUTROS::CIENCIAS: NEUROCIÊNCIASOptogeneticsDorsal cochlear nucleusAuditory systemTinnitusValidation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in miceValidação da expressão de proteínas optogenéticas no Núcleo coclear dorsal: bases moleculares para investigação in vitro e in vivo de tinnitus em camundongosinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALThawannMalfattiBorges_DISSERT.pdfThawannMalfattiBorges_DISSERT.pdfapplication/pdf25181144https://repositorio.ufrn.br/bitstream/123456789/20343/1/ThawannMalfattiBorges_DISSERT.pdf53425dc7e7e3de92d5ef93c6a7dfbe23MD51TEXTThawannMalfattiBorges_DISSERT.pdf.txtThawannMalfattiBorges_DISSERT.pdf.txtExtracted texttext/plain106935https://repositorio.ufrn.br/bitstream/123456789/20343/6/ThawannMalfattiBorges_DISSERT.pdf.txt5fe1bad863b0bde1ed75108eb3154b44MD56THUMBNAILThawannMalfattiBorges_DISSERT.pdf.jpgThawannMalfattiBorges_DISSERT.pdf.jpgIM Thumbnailimage/jpeg2151https://repositorio.ufrn.br/bitstream/123456789/20343/7/ThawannMalfattiBorges_DISSERT.pdf.jpg3ddd5621eaea05e34ca2df94086c8fadMD57123456789/203432017-11-02 15:56:16.518oai:https://repositorio.ufrn.br:123456789/20343Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2017-11-02T18:56:16Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice
dc.title.alternative.pt_BR.fl_str_mv Validação da expressão de proteínas optogenéticas no Núcleo coclear dorsal: bases moleculares para investigação in vitro e in vivo de tinnitus em camundongos
title Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice
spellingShingle Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice
Borges, Thawann Malfatti
CNPQ::OUTROS::CIENCIAS: NEUROCIÊNCIAS
Optogenetics
Dorsal cochlear nucleus
Auditory system
Tinnitus
title_short Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice
title_full Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice
title_fullStr Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice
title_full_unstemmed Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice
title_sort Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice
author Borges, Thawann Malfatti
author_facet Borges, Thawann Malfatti
author_role author
dc.contributor.authorID.pt_BR.fl_str_mv
dc.contributor.authorLattes.none.fl_str_mv http://lattes.cnpq.br/1262934391596208
dc.contributor.advisorID.pt_BR.fl_str_mv
dc.contributor.advisorLattes.none.fl_str_mv http://lattes.cnpq.br/1279823352935722
dc.contributor.referees1.none.fl_str_mv Leão, Ricardo Maurício Xavier
dc.contributor.referees1ID.pt_BR.fl_str_mv
dc.contributor.referees1Lattes.none.fl_str_mv http://lattes.cnpq.br/8125613125649746
dc.contributor.referees2.none.fl_str_mv Leão, Richardson Naves
dc.contributor.referees2ID.pt_BR.fl_str_mv
dc.contributor.referees2Lattes.none.fl_str_mv http://lattes.cnpq.br/0683942077872227
dc.contributor.author.fl_str_mv Borges, Thawann Malfatti
dc.contributor.advisor1.fl_str_mv Leão, Emelie Katarina Svahn
contributor_str_mv Leão, Emelie Katarina Svahn
dc.subject.cnpq.fl_str_mv CNPQ::OUTROS::CIENCIAS: NEUROCIÊNCIAS
topic CNPQ::OUTROS::CIENCIAS: NEUROCIÊNCIAS
Optogenetics
Dorsal cochlear nucleus
Auditory system
Tinnitus
dc.subject.por.fl_str_mv Optogenetics
Dorsal cochlear nucleus
Auditory system
Tinnitus
description Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)
publishDate 2015
dc.date.issued.fl_str_mv 2015-06-26
dc.date.accessioned.fl_str_mv 2016-04-29T20:35:41Z
dc.date.available.fl_str_mv 2016-04-29T20:35:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.citation.fl_str_mv BORGES, Thawann Malfatti. Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice. 2015. 60f. Dissertação (Mestrado em Neurociências) - Universidade Federal do Rio Grande do Norte, Natal, 2015.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/jspui/handle/123456789/20343
identifier_str_mv BORGES, Thawann Malfatti. Validation of optogenetic protein expression in the Dorsal cochlear nucleus: molecular basis for in vitro and in vivo investigation of tinnitus in mice. 2015. 60f. Dissertação (Mestrado em Neurociências) - Universidade Federal do Rio Grande do Norte, Natal, 2015.
url https://repositorio.ufrn.br/jspui/handle/123456789/20343
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Universidade Federal do Rio Grande do Norte
dc.publisher.program.fl_str_mv PROGRAMA DE PÓS-GRADUAÇÃO EM NEUROCIÊNCIAS
dc.publisher.initials.fl_str_mv UFRN
dc.publisher.country.fl_str_mv Brasil
publisher.none.fl_str_mv Universidade Federal do Rio Grande do Norte
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
instname_str Universidade Federal do Rio Grande do Norte (UFRN)
instacron_str UFRN
institution UFRN
reponame_str Repositório Institucional da UFRN
collection Repositório Institucional da UFRN
bitstream.url.fl_str_mv https://repositorio.ufrn.br/bitstream/123456789/20343/1/ThawannMalfattiBorges_DISSERT.pdf
https://repositorio.ufrn.br/bitstream/123456789/20343/6/ThawannMalfattiBorges_DISSERT.pdf.txt
https://repositorio.ufrn.br/bitstream/123456789/20343/7/ThawannMalfattiBorges_DISSERT.pdf.jpg
bitstream.checksum.fl_str_mv 53425dc7e7e3de92d5ef93c6a7dfbe23
5fe1bad863b0bde1ed75108eb3154b44
3ddd5621eaea05e34ca2df94086c8fad
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)
repository.mail.fl_str_mv
_version_ 1802117574349029376

Registros relacionados