Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells
Autor(a) principal: | |
---|---|
Data de Publicação: | 2019 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/28788 |
Resumo: | Edward Tolman postulated the existence of cognitive maps in the brain to explain the animal capability of spatial navigation. Since then, neuroscience seeks to describe brain components underlying this capability. In the present work, we describe advancements in the characterization of a neural sub-population engaged in coding the scalar velocity of rat locomotion. Upon analyzing an open database, we re-discovered the existence of a velocity correlation present in the rate of emission of action potentials of neurons in the dorsal hippocampus. we found that this correlation is independent of theta oscillation frequency (4- 12 Hz) and stable over space and time; moreover, it also persists in different arenas. We then classified the neurons as excitatory and inhibitory by the action potential waveform shapes, the rate of emission of action potentials, and the temporal inter-dependency of action potential emission between pairs of neurons. This classification revealed that, by using Pearson’s r coefficient (speed score) as a correlation metric, in the square open-field arena, only inhibitory neurons high-correlated with locomotion speed (henceforth, speed), even though there was a modulation of the average of the excitatory neurons. Intriguingly, when checking speed correlates in the linear arena, speed score made indistinguishable the correlation among neuronal classes. We then formulated the hypothesis that the apparent locomotion speed correlation of pyramidal neurons in the linear arena is spurious, due to a by-product of their spatial code. To prove this, we simulated artificial neurons that emitted action potentials influenced by the actual animal behavior; the simulated neurons coded either speed or space. The simulation replicated real data Pearson’s r coefficient classifications – true positives in the square arena and false positives in the linear arena for those cells encoding speed. To solve this ethological interdependency, we adopted a new metric of speed correlation – the ratio of the difference of linear-non-linear models prediction accuracy based on speed and position over the prediction accuracy of the two-variable model. This analysis disentangled the ethological issue, satisfactorily classifying the simulated neurons and confirming the spurious hypothesis correlates, and the prevalence of speed correlates in interneurons. The results of the present work demonstrated that a genuine speed correlation is present in the dorsal CA1 of the hippocampus of rats in inhibitory neurons. |
id |
UFRN_06a7316a13a86883b81beb66f6611fe6 |
---|---|
oai_identifier_str |
oai:https://repositorio.ufrn.br:123456789/28788 |
network_acronym_str |
UFRN |
network_name_str |
Repositório Institucional da UFRN |
repository_id_str |
|
spelling |
Góis, José Henrique Targino DiasTakahashi, Daniel YasumasaPereira, Rodrigo Neves RomcyTreptow, WernerFiguerola, Wilfredo BlancoTort, Adriano Bretanha Lopes2020-04-15T18:45:30Z2020-04-15T18:45:30Z2019-12-06GÓIS, José Henrique Targino Dias. Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells. 2019. 70f. Tese (Doutorado em Neurociências) - Instituto do Cérebro, Universidade Federal do Rio Grande do Norte, Natal, 2019.https://repositorio.ufrn.br/jspui/handle/123456789/28788Edward Tolman postulated the existence of cognitive maps in the brain to explain the animal capability of spatial navigation. Since then, neuroscience seeks to describe brain components underlying this capability. In the present work, we describe advancements in the characterization of a neural sub-population engaged in coding the scalar velocity of rat locomotion. Upon analyzing an open database, we re-discovered the existence of a velocity correlation present in the rate of emission of action potentials of neurons in the dorsal hippocampus. we found that this correlation is independent of theta oscillation frequency (4- 12 Hz) and stable over space and time; moreover, it also persists in different arenas. We then classified the neurons as excitatory and inhibitory by the action potential waveform shapes, the rate of emission of action potentials, and the temporal inter-dependency of action potential emission between pairs of neurons. This classification revealed that, by using Pearson’s r coefficient (speed score) as a correlation metric, in the square open-field arena, only inhibitory neurons high-correlated with locomotion speed (henceforth, speed), even though there was a modulation of the average of the excitatory neurons. Intriguingly, when checking speed correlates in the linear arena, speed score made indistinguishable the correlation among neuronal classes. We then formulated the hypothesis that the apparent locomotion speed correlation of pyramidal neurons in the linear arena is spurious, due to a by-product of their spatial code. To prove this, we simulated artificial neurons that emitted action potentials influenced by the actual animal behavior; the simulated neurons coded either speed or space. The simulation replicated real data Pearson’s r coefficient classifications – true positives in the square arena and false positives in the linear arena for those cells encoding speed. To solve this ethological interdependency, we adopted a new metric of speed correlation – the ratio of the difference of linear-non-linear models prediction accuracy based on speed and position over the prediction accuracy of the two-variable model. This analysis disentangled the ethological issue, satisfactorily classifying the simulated neurons and confirming the spurious hypothesis correlates, and the prevalence of speed correlates in interneurons. The results of the present work demonstrated that a genuine speed correlation is present in the dorsal CA1 of the hippocampus of rats in inhibitory neurons.Edward Tolman postulou a existência de mapas cognitivos no cérebro para explicar a capacidade animal de navegar pelo espaço. Desde então, neurocientistas buscam descrever componentes cerebrais que suportam essa capacidade. No presente trabalho, descrevemos avanços na caracterização de uma sub-população neural que participa na representação da velocidade escalar na locomoção de ratos. Através da análise de um banco de dados aberto, nós re-descobrimos a existência do correlato de velocidade na taxa de emissão de potenciais de ação de neurônios da região CA1 do hipocampo dorsal de ratos. Essa correlação é independente da frequência da oscilação de theta (4-12 Hz) e é estável através do espaço e tempo; além disso, essa correlação persiste em diferentes arenas. A classificação de neurônios entre prováveis excitatórios ou inibitórios revelou que, ao utilizar o coeficiente r de Pearson (índice de velocidade) como métrica de representação de velocidade nos neurônios, somente neurônios inibitórios apresentaram-se como altamente correlacionados com a velocidade na arena quadrada, embora os neurônios excitatórios apresentem uma modulação na média da taxa de emissão de potenciais de ação. Intrigantemente, na arena linear a mesma métrica tornou indistinguiveis as classificações neurais pela correlação com a velocidade. Então, formulamos a hipótese que a aparente correlação com a velocidade dos neurônios excitatórios na arena linear é espúria – um subproduto da correlação de velocidade com o espaço. Para testar essa hipótese, simulei neurônios artificiais que emitem potenciais de ação influenciados pelo comportamento locomotor do animal; essas simulações codificam velocidade ou espaço. As simulações replicaram a classificação pelo índice de velocidade encontrados nos dados reais – ou seja, código de posição não correlaciona com velocidade na arena quadrada mas sim na arena linear. Para solucionar essa interdependência etológica, nós adotamos uma nova métrica de correlação com a velocidade: a razão da diferença da acurácia de predição dos modelos linear-não-linear baseados em velocidade e posição sobre a acurácia de predição do modelo das duas variáveis. Essa análise resolveu o problema etológico, classificou satisfatoriamente as simulações e confirmou a hipótese da correlação espúria na arena linear e a prevalencia da correlação nos neurônios inibitórios. Os resultados deste trabalho demonstra uma correlação genuina da velocidade de locomoção nos neurônios inibitórios na área CA1 do hipocampo dorsal de ratos.Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPqCoordenação de Aperfeiçoamento de Pessoal de Nível Superior - CAPESCNPQ::OUTROS::CIENCIASSpatial navigationLocomotion speedRatsHippocampusNeuronsElectrophysiologyNeuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cellsCorrelatos neuronais da velocidade de locomoção em CA1 dorsal do hipocampo de ratos: novas analises nas células de velocidadeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisPROGRAMA DE PÓS-GRADUAÇÃO EM NEUROCIÊNCIASUFRNBrasilinfo:eu-repo/semantics/openAccessporreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNTEXTNeuronalcorrelateslocomotion_Gois_2019.pdf.txtNeuronalcorrelateslocomotion_Gois_2019.pdf.txtExtracted texttext/plain141491https://repositorio.ufrn.br/bitstream/123456789/28788/2/Neuronalcorrelateslocomotion_Gois_2019.pdf.txtfbd892c1c8eb9ef7db461ab896aceb7eMD52THUMBNAILNeuronalcorrelateslocomotion_Gois_2019.pdf.jpgNeuronalcorrelateslocomotion_Gois_2019.pdf.jpgGenerated Thumbnailimage/jpeg1456https://repositorio.ufrn.br/bitstream/123456789/28788/3/Neuronalcorrelateslocomotion_Gois_2019.pdf.jpgb8958b3a5492f7f838eabbbe4c58994aMD53ORIGINALNeuronalcorrelateslocomotion_Gois_2019.pdfapplication/pdf8298155https://repositorio.ufrn.br/bitstream/123456789/28788/1/Neuronalcorrelateslocomotion_Gois_2019.pdfb4de91c822561731c16d3a9b0acfa53aMD51123456789/287882020-04-19 04:47:07.241oai:https://repositorio.ufrn.br:123456789/28788Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2020-04-19T07:47:07Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells |
dc.title.alternative.pt_BR.fl_str_mv |
Correlatos neuronais da velocidade de locomoção em CA1 dorsal do hipocampo de ratos: novas analises nas células de velocidade |
title |
Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells |
spellingShingle |
Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells Góis, José Henrique Targino Dias CNPQ::OUTROS::CIENCIAS Spatial navigation Locomotion speed Rats Hippocampus Neurons Electrophysiology |
title_short |
Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells |
title_full |
Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells |
title_fullStr |
Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells |
title_full_unstemmed |
Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells |
title_sort |
Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells |
author |
Góis, José Henrique Targino Dias |
author_facet |
Góis, José Henrique Targino Dias |
author_role |
author |
dc.contributor.authorID.pt_BR.fl_str_mv |
|
dc.contributor.advisorID.pt_BR.fl_str_mv |
|
dc.contributor.referees1.none.fl_str_mv |
Takahashi, Daniel Yasumasa |
dc.contributor.referees1ID.pt_BR.fl_str_mv |
|
dc.contributor.referees2.none.fl_str_mv |
Pereira, Rodrigo Neves Romcy |
dc.contributor.referees2ID.pt_BR.fl_str_mv |
|
dc.contributor.referees3.none.fl_str_mv |
Treptow, Werner |
dc.contributor.referees3ID.pt_BR.fl_str_mv |
|
dc.contributor.referees4.none.fl_str_mv |
Figuerola, Wilfredo Blanco |
dc.contributor.referees4ID.pt_BR.fl_str_mv |
|
dc.contributor.author.fl_str_mv |
Góis, José Henrique Targino Dias |
dc.contributor.advisor1.fl_str_mv |
Tort, Adriano Bretanha Lopes |
contributor_str_mv |
Tort, Adriano Bretanha Lopes |
dc.subject.cnpq.fl_str_mv |
CNPQ::OUTROS::CIENCIAS |
topic |
CNPQ::OUTROS::CIENCIAS Spatial navigation Locomotion speed Rats Hippocampus Neurons Electrophysiology |
dc.subject.por.fl_str_mv |
Spatial navigation Locomotion speed Rats Hippocampus Neurons Electrophysiology |
description |
Edward Tolman postulated the existence of cognitive maps in the brain to explain the animal capability of spatial navigation. Since then, neuroscience seeks to describe brain components underlying this capability. In the present work, we describe advancements in the characterization of a neural sub-population engaged in coding the scalar velocity of rat locomotion. Upon analyzing an open database, we re-discovered the existence of a velocity correlation present in the rate of emission of action potentials of neurons in the dorsal hippocampus. we found that this correlation is independent of theta oscillation frequency (4- 12 Hz) and stable over space and time; moreover, it also persists in different arenas. We then classified the neurons as excitatory and inhibitory by the action potential waveform shapes, the rate of emission of action potentials, and the temporal inter-dependency of action potential emission between pairs of neurons. This classification revealed that, by using Pearson’s r coefficient (speed score) as a correlation metric, in the square open-field arena, only inhibitory neurons high-correlated with locomotion speed (henceforth, speed), even though there was a modulation of the average of the excitatory neurons. Intriguingly, when checking speed correlates in the linear arena, speed score made indistinguishable the correlation among neuronal classes. We then formulated the hypothesis that the apparent locomotion speed correlation of pyramidal neurons in the linear arena is spurious, due to a by-product of their spatial code. To prove this, we simulated artificial neurons that emitted action potentials influenced by the actual animal behavior; the simulated neurons coded either speed or space. The simulation replicated real data Pearson’s r coefficient classifications – true positives in the square arena and false positives in the linear arena for those cells encoding speed. To solve this ethological interdependency, we adopted a new metric of speed correlation – the ratio of the difference of linear-non-linear models prediction accuracy based on speed and position over the prediction accuracy of the two-variable model. This analysis disentangled the ethological issue, satisfactorily classifying the simulated neurons and confirming the spurious hypothesis correlates, and the prevalence of speed correlates in interneurons. The results of the present work demonstrated that a genuine speed correlation is present in the dorsal CA1 of the hippocampus of rats in inhibitory neurons. |
publishDate |
2019 |
dc.date.issued.fl_str_mv |
2019-12-06 |
dc.date.accessioned.fl_str_mv |
2020-04-15T18:45:30Z |
dc.date.available.fl_str_mv |
2020-04-15T18:45:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GÓIS, José Henrique Targino Dias. Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells. 2019. 70f. Tese (Doutorado em Neurociências) - Instituto do Cérebro, Universidade Federal do Rio Grande do Norte, Natal, 2019. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/28788 |
identifier_str_mv |
GÓIS, José Henrique Targino Dias. Neuronal correlates of locomotion speed in the dorsal CA1 of the rat hippocampus: new insights on the speed cells. 2019. 70f. Tese (Doutorado em Neurociências) - Instituto do Cérebro, Universidade Federal do Rio Grande do Norte, Natal, 2019. |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/28788 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.program.fl_str_mv |
PROGRAMA DE PÓS-GRADUAÇÃO EM NEUROCIÊNCIAS |
dc.publisher.initials.fl_str_mv |
UFRN |
dc.publisher.country.fl_str_mv |
Brasil |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
instname_str |
Universidade Federal do Rio Grande do Norte (UFRN) |
instacron_str |
UFRN |
institution |
UFRN |
reponame_str |
Repositório Institucional da UFRN |
collection |
Repositório Institucional da UFRN |
bitstream.url.fl_str_mv |
https://repositorio.ufrn.br/bitstream/123456789/28788/2/Neuronalcorrelateslocomotion_Gois_2019.pdf.txt https://repositorio.ufrn.br/bitstream/123456789/28788/3/Neuronalcorrelateslocomotion_Gois_2019.pdf.jpg https://repositorio.ufrn.br/bitstream/123456789/28788/1/Neuronalcorrelateslocomotion_Gois_2019.pdf |
bitstream.checksum.fl_str_mv |
fbd892c1c8eb9ef7db461ab896aceb7e b8958b3a5492f7f838eabbbe4c58994a b4de91c822561731c16d3a9b0acfa53a |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN) |
repository.mail.fl_str_mv |
|
_version_ |
1802117877213429760 |