Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction

Detalhes bibliográficos
Autor(a) principal: Courtial, Camila Gambini Pereira
Data de Publicação: 2006
Outros Autores: Carvalho, J. E., Meireles, Maria Angela de Almeida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32550
Resumo: In the present work the anticancer of crude supercritical fluid extract from T. catharinensis was evaluated. The crude extract was obtained using supercritical CO² at pressure of 300 bar and temperature of 55º C; ethanol was used as cosolvent (4.6% mass). The anticancer activity was tested against seven human cancer cell lines: lung, breast, breast expressing rhe multidrug resistance phenotype, melanoma, colon, prostate, and, kidney. The crude extract was fractionated and the alkaloidal fraction was analyzed by thin-layer chromatografy (TLC), and gas-chromatography/flame ionization detector (GC-FID). The GC-FID of the alkaloidal fraction indicated the presence of the indole alkaloids voacangine and coronaridine. The crude extract of T. catharinensis has anticancer activity that begins at 0.25 ug/mL and exhibits cytostatic and cytolytic effects concentration dependent and noy selective for the cell lines used
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spelling Courtial, Camila Gambini PereiraCarvalho, J. E.Meireles, Maria Angela de Almeida2021-05-21T19:42:28Z2021-05-21T19:42:28Z2006-10-31PEREIRA, C. G.; CARVALHO, J.E. ; MEIRELES, M.A.A.. Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction. Revista Brasileira de Plantas Medicinais, v. 8, p. 144-149, 2006. Disponível em: https://www1.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo28_v8_n4_p144-149.pdf. Acesso em 08 abr. 2021.1516-05721983-084Xhttps://repositorio.ufrn.br/handle/123456789/32550In the present work the anticancer of crude supercritical fluid extract from T. catharinensis was evaluated. The crude extract was obtained using supercritical CO² at pressure of 300 bar and temperature of 55º C; ethanol was used as cosolvent (4.6% mass). The anticancer activity was tested against seven human cancer cell lines: lung, breast, breast expressing rhe multidrug resistance phenotype, melanoma, colon, prostate, and, kidney. The crude extract was fractionated and the alkaloidal fraction was analyzed by thin-layer chromatografy (TLC), and gas-chromatography/flame ionization detector (GC-FID). The GC-FID of the alkaloidal fraction indicated the presence of the indole alkaloids voacangine and coronaridine. The crude extract of T. catharinensis has anticancer activity that begins at 0.25 ug/mL and exhibits cytostatic and cytolytic effects concentration dependent and noy selective for the cell lines usedNo presente trabalho a atividade do anticâncer do extrato bruto do fluido supercrítico de T. catharinensis foi avaliada. O extrato bruto foi obtido utilizando CO² supercrítico na pressão de 300 bar e temperatura de 55º C; etanol foi usado como co-solvente (4,6% massa). A atividade anticâncer foi testada contra sete linhagens de tumores humanas: pulmão, mama, mama expressando fenótipo resistente a múltiplas drogas, melanoma, cólon, próstata e rim. O extrato bruto foi fracionado e a fração alcaloídica foi analisada por cromatografia em camada delgada (CGD) e cromatografia a gás com detector por ionização em chama (CG-DIC). A CG-DIC da fração alcaloídica indicou a presença dos alcalóides indólicos voacangina e coronaridina. O extrato bruto de T. catharinensis apresentou atividade anticâncer a partir de 0,25 ug / mL com efeito citostático e citolítico, dependente da concentração e sem seletividade entre as linhagens celulares utilizadasSociedade Brasileira de Plantas MedicinaisAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessTabernaemontana catharinensisIndole alkaloidsSupercritical fluid extractionAnticancer activityAnticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extractioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALAnticancerActivity_PEREIRA_2006.pdfAnticancerActivity_PEREIRA_2006.pdfapplication/pdf138283https://repositorio.ufrn.br/bitstream/123456789/32550/1/AnticancerActivity_PEREIRA_2006.pdfea4fe38e72df0fed519976c7cd306a88MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32550/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32550/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/325502021-05-21 16:42:30.43oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-21T19:42:30Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction
title Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction
spellingShingle Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction
Courtial, Camila Gambini Pereira
Tabernaemontana catharinensis
Indole alkaloids
Supercritical fluid extraction
Anticancer activity
title_short Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction
title_full Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction
title_fullStr Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction
title_full_unstemmed Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction
title_sort Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction
author Courtial, Camila Gambini Pereira
author_facet Courtial, Camila Gambini Pereira
Carvalho, J. E.
Meireles, Maria Angela de Almeida
author_role author
author2 Carvalho, J. E.
Meireles, Maria Angela de Almeida
author2_role author
author
dc.contributor.author.fl_str_mv Courtial, Camila Gambini Pereira
Carvalho, J. E.
Meireles, Maria Angela de Almeida
dc.subject.por.fl_str_mv Tabernaemontana catharinensis
Indole alkaloids
Supercritical fluid extraction
Anticancer activity
topic Tabernaemontana catharinensis
Indole alkaloids
Supercritical fluid extraction
Anticancer activity
description In the present work the anticancer of crude supercritical fluid extract from T. catharinensis was evaluated. The crude extract was obtained using supercritical CO² at pressure of 300 bar and temperature of 55º C; ethanol was used as cosolvent (4.6% mass). The anticancer activity was tested against seven human cancer cell lines: lung, breast, breast expressing rhe multidrug resistance phenotype, melanoma, colon, prostate, and, kidney. The crude extract was fractionated and the alkaloidal fraction was analyzed by thin-layer chromatografy (TLC), and gas-chromatography/flame ionization detector (GC-FID). The GC-FID of the alkaloidal fraction indicated the presence of the indole alkaloids voacangine and coronaridine. The crude extract of T. catharinensis has anticancer activity that begins at 0.25 ug/mL and exhibits cytostatic and cytolytic effects concentration dependent and noy selective for the cell lines used
publishDate 2006
dc.date.issued.fl_str_mv 2006-10-31
dc.date.accessioned.fl_str_mv 2021-05-21T19:42:28Z
dc.date.available.fl_str_mv 2021-05-21T19:42:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, C. G.; CARVALHO, J.E. ; MEIRELES, M.A.A.. Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction. Revista Brasileira de Plantas Medicinais, v. 8, p. 144-149, 2006. Disponível em: https://www1.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo28_v8_n4_p144-149.pdf. Acesso em 08 abr. 2021.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32550
dc.identifier.issn.none.fl_str_mv 1516-0572
1983-084X
identifier_str_mv PEREIRA, C. G.; CARVALHO, J.E. ; MEIRELES, M.A.A.. Anticancer activity of Tabernaemontana catharinensis extract obtained by supercritical fluid extraction. Revista Brasileira de Plantas Medicinais, v. 8, p. 144-149, 2006. Disponível em: https://www1.ibb.unesp.br/Home/Departamentos/Botanica/RBPM-RevistaBrasileiradePlantasMedicinais/artigo28_v8_n4_p144-149.pdf. Acesso em 08 abr. 2021.
1516-0572
1983-084X
url https://repositorio.ufrn.br/handle/123456789/32550
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Plantas Medicinais
publisher.none.fl_str_mv Sociedade Brasileira de Plantas Medicinais
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
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