Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis

Detalhes bibliográficos
Autor(a) principal: Soares, Deivid Costa
Data de Publicação: 2007
Outros Autores: Courtial, Camila Gambini Pereira, Meireles, Maria Angela de Almeida, Saraiva, Elvira Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFRN
Texto Completo: https://repositorio.ufrn.br/handle/123456789/32549
Resumo: The branches and leaves of Tabernaemontana catharinensis were extracted with supercritical fluid using a mixture of CO2 plus ethanol (SFE), and the indole alkaloid enriched fraction (AF3) was selected for anti-Leishmania activity studies. We found that AF3 exhibits a potent effect against intracellular amastigotes of Leishmania amazonensis, a causative agent of New World cutaneous leishmaniasis. AF3 inhibits Leishmania survival in a dose-dependent manner, and reached 88% inhibition of amastigote growth at 100 μg/mL. The anti-parasite effect was independent of nitric oxide (NO), since AF3 was able to inhibit NO production induced by IFN-γ plus LPS. In addition, AF3 inhibited TGF-β production, which could have facilitated AF3-mediated parasite killing. The AF3 fraction obtained from SFE was nontoxic for host macrophages, as assessed by plasma membrane integrity and mitochondrial activity. We conclude that SFE is an efficient method for obtaining bioactive indole alkaloids from plant extracts. Importantly, this method preserved the alkaloid properties associated with inhibition of Leishmania growth in macrophages without toxicity to host cells
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spelling Soares, Deivid CostaCourtial, Camila Gambini PereiraMeireles, Maria Angela de AlmeidaSaraiva, Elvira Maria2021-05-21T19:22:12Z2021-05-21T19:22:12Z2007-06SOARES, D. C.; PEREIRA, C. G.; MEIRELES, M.A.A.; SARAIVA, E. M.. Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis. Parasitology International, v. 56, p. 135-139, 2007. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1383576907000074?via%3Dihub. Acesso em: 08 abr. 2021. https://doi.org/10.1016/j.parint.2007.01.004.1383-5769https://repositorio.ufrn.br/handle/123456789/3254910.1016/j.parint.2007.01.004ElsevierAttribution 3.0 Brazilhttp://creativecommons.org/licenses/by/3.0/br/info:eu-repo/semantics/openAccessLeishmanicidal activityTabernaemontanaLeishmaniaSupercritical Fluid ExtractionLeishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe branches and leaves of Tabernaemontana catharinensis were extracted with supercritical fluid using a mixture of CO2 plus ethanol (SFE), and the indole alkaloid enriched fraction (AF3) was selected for anti-Leishmania activity studies. We found that AF3 exhibits a potent effect against intracellular amastigotes of Leishmania amazonensis, a causative agent of New World cutaneous leishmaniasis. AF3 inhibits Leishmania survival in a dose-dependent manner, and reached 88% inhibition of amastigote growth at 100 μg/mL. The anti-parasite effect was independent of nitric oxide (NO), since AF3 was able to inhibit NO production induced by IFN-γ plus LPS. In addition, AF3 inhibited TGF-β production, which could have facilitated AF3-mediated parasite killing. The AF3 fraction obtained from SFE was nontoxic for host macrophages, as assessed by plasma membrane integrity and mitochondrial activity. We conclude that SFE is an efficient method for obtaining bioactive indole alkaloids from plant extracts. Importantly, this method preserved the alkaloid properties associated with inhibition of Leishmania growth in macrophages without toxicity to host cellsengreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNORIGINALLeishmanicidalActivitySupercritical_PEREIRA_2007.pdfLeishmanicidalActivitySupercritical_PEREIRA_2007.pdfArtigoapplication/pdf262524https://repositorio.ufrn.br/bitstream/123456789/32549/1/LeishmanicidalActivitySupercritical_PEREIRA_2007.pdf0f1b897910266aa73db4d5659664cde4MD51CC-LICENSElicense_rdflicense_rdfapplication/rdf+xml; charset=utf-8914https://repositorio.ufrn.br/bitstream/123456789/32549/2/license_rdf4d2950bda3d176f570a9f8b328dfbbefMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81484https://repositorio.ufrn.br/bitstream/123456789/32549/3/license.txte9597aa2854d128fd968be5edc8a28d9MD53123456789/325492021-05-21 16:22:13.88oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-05-21T19:22:13Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false
dc.title.pt_BR.fl_str_mv Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
title Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
spellingShingle Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
Soares, Deivid Costa
Leishmanicidal activity
Tabernaemontana
Leishmania
Supercritical Fluid Extraction
title_short Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
title_full Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
title_fullStr Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
title_full_unstemmed Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
title_sort Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis
author Soares, Deivid Costa
author_facet Soares, Deivid Costa
Courtial, Camila Gambini Pereira
Meireles, Maria Angela de Almeida
Saraiva, Elvira Maria
author_role author
author2 Courtial, Camila Gambini Pereira
Meireles, Maria Angela de Almeida
Saraiva, Elvira Maria
author2_role author
author
author
dc.contributor.author.fl_str_mv Soares, Deivid Costa
Courtial, Camila Gambini Pereira
Meireles, Maria Angela de Almeida
Saraiva, Elvira Maria
dc.subject.por.fl_str_mv Leishmanicidal activity
Tabernaemontana
Leishmania
Supercritical Fluid Extraction
topic Leishmanicidal activity
Tabernaemontana
Leishmania
Supercritical Fluid Extraction
description The branches and leaves of Tabernaemontana catharinensis were extracted with supercritical fluid using a mixture of CO2 plus ethanol (SFE), and the indole alkaloid enriched fraction (AF3) was selected for anti-Leishmania activity studies. We found that AF3 exhibits a potent effect against intracellular amastigotes of Leishmania amazonensis, a causative agent of New World cutaneous leishmaniasis. AF3 inhibits Leishmania survival in a dose-dependent manner, and reached 88% inhibition of amastigote growth at 100 μg/mL. The anti-parasite effect was independent of nitric oxide (NO), since AF3 was able to inhibit NO production induced by IFN-γ plus LPS. In addition, AF3 inhibited TGF-β production, which could have facilitated AF3-mediated parasite killing. The AF3 fraction obtained from SFE was nontoxic for host macrophages, as assessed by plasma membrane integrity and mitochondrial activity. We conclude that SFE is an efficient method for obtaining bioactive indole alkaloids from plant extracts. Importantly, this method preserved the alkaloid properties associated with inhibition of Leishmania growth in macrophages without toxicity to host cells
publishDate 2007
dc.date.issued.fl_str_mv 2007-06
dc.date.accessioned.fl_str_mv 2021-05-21T19:22:12Z
dc.date.available.fl_str_mv 2021-05-21T19:22:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, D. C.; PEREIRA, C. G.; MEIRELES, M.A.A.; SARAIVA, E. M.. Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis. Parasitology International, v. 56, p. 135-139, 2007. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1383576907000074?via%3Dihub. Acesso em: 08 abr. 2021. https://doi.org/10.1016/j.parint.2007.01.004.
dc.identifier.uri.fl_str_mv https://repositorio.ufrn.br/handle/123456789/32549
dc.identifier.issn.none.fl_str_mv 1383-5769
dc.identifier.doi.none.fl_str_mv 10.1016/j.parint.2007.01.004
identifier_str_mv SOARES, D. C.; PEREIRA, C. G.; MEIRELES, M.A.A.; SARAIVA, E. M.. Leishmanicidal activity of a supercritical fluid fraction obtained from Tabernaemontana catharinensis. Parasitology International, v. 56, p. 135-139, 2007. Disponível em: https://www.sciencedirect.com/science/article/abs/pii/S1383576907000074?via%3Dihub. Acesso em: 08 abr. 2021. https://doi.org/10.1016/j.parint.2007.01.004.
1383-5769
10.1016/j.parint.2007.01.004
url https://repositorio.ufrn.br/handle/123456789/32549
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 3.0 Brazil
http://creativecommons.org/licenses/by/3.0/br/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFRN
instname:Universidade Federal do Rio Grande do Norte (UFRN)
instacron:UFRN
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