Recovery and purification of chitosanaseproduced by Bacillus cereus using expandedbed adsorption and central composite design

• Autor(a) principal: Santos, Everaldo Silvino dos
• Data de Publicação: 2016
• Outros Autores: Macedo, Gorete Ribeiro de, Araújo, Nathália Kelly de, Pimentel, Vanessa Carvalho, Silva, Nayane Macedo Portela da, Padilha, Carlos Eduardo de Araújo
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UFRN
• DOI: 10.1002/jssc.201500900
• Texto Completo: https://repositorio.ufrn.br/handle/123456789/32319
• topic: Bioseparation; Chitosanase; Downstream Processing; Expanded bedadsorption

This study presents a system for expanded bed adsorption for the purification of chitosanasefrom broth extract in a single step. A chitosanase-producing strain was isolated and identifiedas Bacillus cereus C-01 and used to produce chitosanases. The expanded bed adsorptionconditions for chitosanase purification were optimized statistically using STREAMLINETMDEAE and a homemade column (2.6 × 30.0 cm). Dependent variables were defined by thequality criteria purification factor (P) and enzyme yield to optimize the chromatographicprocess. Statistical analyses showed that the optimum conditions for the maximum P were150 cm/h load flow velocity, 6.0 cm settled bed height, and 7.36 cm distributor height.Distributor height had a strong influence on the process, considerably affecting both theP and enzyme yield. Optimizing the purification variables resulted in an approximately3.66-fold increase in the P compared with the value under nonoptimized conditions. Thissystem is promising for the recovery of chitosanase from B. cereus C-01 and is economicallyviable because it promotes the reduction steps