Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRN |
Texto Completo: | https://repositorio.ufrn.br/jspui/handle/123456789/25413 http://dx.doi.org/10.3748/wjg.v18.i31.4162 |
Resumo: | AIM: To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell lines. METHODS: Colorectal carcinoma (HT29) and human hepatocellular carcinoma (HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri (SDEPN) either alone or in combination with cisplatin at different concentrations (0.5 mg/mL and 1 mg/mL) for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with propidium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histograms. Significant differences between groups were determined using analysis of variance and Bonferroni’s test, as indicated. A value of P < 0.05 was considered to be statistically significant. RESULTS: SDEPN had significantly different cytotoxic effects on HT29 (2.81 ± 0.11 vs 3.51 ± 1.13, P > 0.05) and HepG2 (5.07 ± 0.3 vs 15.9 ± 1.04, P < 0.001) cells when compared to control cells for 4 h. SDEPN also had significantly different cytotoxic effects on HT29 (1.91 ± 0.57 vs 4.53 ± 1.22, P > 0.05) and HepG2 (14.56 ± 1.6 vs 35.67 ± 3.94, P < 0.001) cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells (HepG2 cells for 4 h: 10.78 ± 1.58 vs 53.89 ± 1.53, P < 0.001; 24 h: 8.9 ± 1.43 vs 62.78 ± 1.87, P < 0.001 and HT29 cells for 4 h: 9.52 ± 0.913 vs 49.86 ± 2.89, P < 0.001; 24 h: 11.78 ± 1.05 vs 53.34 ± 2.65, P < 0.001). In HT29 cells, pretreatment with SDEPN and subsequent treatment with cisplatin resulted in a greater number of cells being killed (12.78 ± 1.01 vs 93.76 ± 1.6, P < 0.001). HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin (12.87 ± 2.78 vs 78.8 ± 3.02, P < 0.001). CONCLUSION: SDEPN is selectively toxic against two cancer cell lines. Moreover, SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells. |
id |
UFRN_3224f592328f22577331b6da4f0922b8 |
---|---|
oai_identifier_str |
oai:https://repositorio.ufrn.br:123456789/25413 |
network_acronym_str |
UFRN |
network_name_str |
Repositório Institucional da UFRN |
repository_id_str |
|
spelling |
Araújo, Aurigena Antunes deAraújo Júnior, Raimundo Fernandes deSoares, Luiz Alberto LiraPorto, Cínthia Raquel da CostaAquino, Ranniere Gurgel Furtado deGuedes, Hugo GonçaloPetrovick, Pedro RosSouza, Tatiane Pereira deGuerra, Gerlane Coelho Bernardo2018-06-16T11:29:20Z2018-06-16T11:29:20Z2012-08-21ARAÚJO, Aurigena Antunes de et al. Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines. World Journal of Gastroenterology, v. 18, p. 4162-4168, 2012. Disponível em: <https://www.wjgnet.com/1007-9327/full/v18/i31/4162.htm>. Acesso em: 14 mar. 2018.2219-2840https://repositorio.ufrn.br/jspui/handle/123456789/25413http://dx.doi.org/10.3748/wjg.v18.i31.4162porBaishidengCisplatinColorectal cancerLiver cancerPhyllanthus niruriCytotoxic effectGrowth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell linesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAIM: To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell lines. METHODS: Colorectal carcinoma (HT29) and human hepatocellular carcinoma (HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri (SDEPN) either alone or in combination with cisplatin at different concentrations (0.5 mg/mL and 1 mg/mL) for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with propidium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histograms. Significant differences between groups were determined using analysis of variance and Bonferroni’s test, as indicated. A value of P < 0.05 was considered to be statistically significant. RESULTS: SDEPN had significantly different cytotoxic effects on HT29 (2.81 ± 0.11 vs 3.51 ± 1.13, P > 0.05) and HepG2 (5.07 ± 0.3 vs 15.9 ± 1.04, P < 0.001) cells when compared to control cells for 4 h. SDEPN also had significantly different cytotoxic effects on HT29 (1.91 ± 0.57 vs 4.53 ± 1.22, P > 0.05) and HepG2 (14.56 ± 1.6 vs 35.67 ± 3.94, P < 0.001) cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells (HepG2 cells for 4 h: 10.78 ± 1.58 vs 53.89 ± 1.53, P < 0.001; 24 h: 8.9 ± 1.43 vs 62.78 ± 1.87, P < 0.001 and HT29 cells for 4 h: 9.52 ± 0.913 vs 49.86 ± 2.89, P < 0.001; 24 h: 11.78 ± 1.05 vs 53.34 ± 2.65, P < 0.001). In HT29 cells, pretreatment with SDEPN and subsequent treatment with cisplatin resulted in a greater number of cells being killed (12.78 ± 1.01 vs 93.76 ± 1.6, P < 0.001). HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin (12.87 ± 2.78 vs 78.8 ± 3.02, P < 0.001). CONCLUSION: SDEPN is selectively toxic against two cancer cell lines. Moreover, SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRNinstname:Universidade Federal do Rio Grande do Norte (UFRN)instacron:UFRNTEXTGrowth inhibitory effects of Phyllanthus niruri_2012.pdf.txtGrowth inhibitory effects of Phyllanthus niruri_2012.pdf.txtExtracted texttext/plain32150https://repositorio.ufrn.br/bitstream/123456789/25413/3/Growth%20inhibitory%20effects%20of%20Phyllanthus%20niruri_2012.pdf.txt889d9ec68ce3b2dbb280f891872268a1MD53THUMBNAILGrowth inhibitory effects of Phyllanthus niruri_2012.pdf.jpgGrowth inhibitory effects of Phyllanthus niruri_2012.pdf.jpgIM Thumbnailimage/jpeg10777https://repositorio.ufrn.br/bitstream/123456789/25413/4/Growth%20inhibitory%20effects%20of%20Phyllanthus%20niruri_2012.pdf.jpgf389410f728a8fa280878e14ddd1f2e3MD54ORIGINALGrowthInhibitoryEffects_Araujo_2012.pdfGrowthInhibitoryEffects_Araujo_2012.pdfapplication/pdf896001https://repositorio.ufrn.br/bitstream/123456789/25413/1/GrowthInhibitoryEffects_Araujo_2012.pdf33a56cd3f7e51af7595f1d72d3de95a2MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81748https://repositorio.ufrn.br/bitstream/123456789/25413/2/license.txt8a4605be74aa9ea9d79846c1fba20a33MD52123456789/254132021-11-11 17:07:21.395oai:https://repositorio.ufrn.br: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Repositório de PublicaçõesPUBhttp://repositorio.ufrn.br/oai/opendoar:2021-11-11T20:07:21Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN)false |
dc.title.pt_BR.fl_str_mv |
Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines |
title |
Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines |
spellingShingle |
Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines Araújo, Aurigena Antunes de Cisplatin Colorectal cancer Liver cancer Phyllanthus niruri Cytotoxic effect |
title_short |
Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines |
title_full |
Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines |
title_fullStr |
Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines |
title_full_unstemmed |
Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines |
title_sort |
Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines |
author |
Araújo, Aurigena Antunes de |
author_facet |
Araújo, Aurigena Antunes de Araújo Júnior, Raimundo Fernandes de Soares, Luiz Alberto Lira Porto, Cínthia Raquel da Costa Aquino, Ranniere Gurgel Furtado de Guedes, Hugo Gonçalo Petrovick, Pedro Ros Souza, Tatiane Pereira de Guerra, Gerlane Coelho Bernardo |
author_role |
author |
author2 |
Araújo Júnior, Raimundo Fernandes de Soares, Luiz Alberto Lira Porto, Cínthia Raquel da Costa Aquino, Ranniere Gurgel Furtado de Guedes, Hugo Gonçalo Petrovick, Pedro Ros Souza, Tatiane Pereira de Guerra, Gerlane Coelho Bernardo |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Araújo, Aurigena Antunes de Araújo Júnior, Raimundo Fernandes de Soares, Luiz Alberto Lira Porto, Cínthia Raquel da Costa Aquino, Ranniere Gurgel Furtado de Guedes, Hugo Gonçalo Petrovick, Pedro Ros Souza, Tatiane Pereira de Guerra, Gerlane Coelho Bernardo |
dc.subject.por.fl_str_mv |
Cisplatin Colorectal cancer Liver cancer Phyllanthus niruri Cytotoxic effect |
topic |
Cisplatin Colorectal cancer Liver cancer Phyllanthus niruri Cytotoxic effect |
description |
AIM: To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell lines. METHODS: Colorectal carcinoma (HT29) and human hepatocellular carcinoma (HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri (SDEPN) either alone or in combination with cisplatin at different concentrations (0.5 mg/mL and 1 mg/mL) for 4 h and 24 h. To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability, we stained the cells with propidium iodide and assessed them by flow cytometry. The percentage of cells in different cell cycle phases was quantified and data were expressed as histograms. Significant differences between groups were determined using analysis of variance and Bonferroni’s test, as indicated. A value of P < 0.05 was considered to be statistically significant. RESULTS: SDEPN had significantly different cytotoxic effects on HT29 (2.81 ± 0.11 vs 3.51 ± 1.13, P > 0.05) and HepG2 (5.07 ± 0.3 vs 15.9 ± 1.04, P < 0.001) cells when compared to control cells for 4 h. SDEPN also had significantly different cytotoxic effects on HT29 (1.91 ± 0.57 vs 4.53 ± 1.22, P > 0.05) and HepG2 (14.56 ± 1.6 vs 35.67 ± 3.94, P < 0.001) cells when compared to control cells for 24 h. Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells (HepG2 cells for 4 h: 10.78 ± 1.58 vs 53.89 ± 1.53, P < 0.001; 24 h: 8.9 ± 1.43 vs 62.78 ± 1.87, P < 0.001 and HT29 cells for 4 h: 9.52 ± 0.913 vs 49.86 ± 2.89, P < 0.001; 24 h: 11.78 ± 1.05 vs 53.34 ± 2.65, P < 0.001). In HT29 cells, pretreatment with SDEPN and subsequent treatment with cisplatin resulted in a greater number of cells being killed (12.78 ± 1.01 vs 93.76 ± 1.6, P < 0.001). HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin (12.87 ± 2.78 vs 78.8 ± 3.02, P < 0.001). CONCLUSION: SDEPN is selectively toxic against two cancer cell lines. Moreover, SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012-08-21 |
dc.date.accessioned.fl_str_mv |
2018-06-16T11:29:20Z |
dc.date.available.fl_str_mv |
2018-06-16T11:29:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
ARAÚJO, Aurigena Antunes de et al. Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines. World Journal of Gastroenterology, v. 18, p. 4162-4168, 2012. Disponível em: <https://www.wjgnet.com/1007-9327/full/v18/i31/4162.htm>. Acesso em: 14 mar. 2018. |
dc.identifier.uri.fl_str_mv |
https://repositorio.ufrn.br/jspui/handle/123456789/25413 |
dc.identifier.issn.none.fl_str_mv |
2219-2840 |
dc.identifier.doi.none.fl_str_mv |
http://dx.doi.org/10.3748/wjg.v18.i31.4162 |
identifier_str_mv |
ARAÚJO, Aurigena Antunes de et al. Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines. World Journal of Gastroenterology, v. 18, p. 4162-4168, 2012. Disponível em: <https://www.wjgnet.com/1007-9327/full/v18/i31/4162.htm>. Acesso em: 14 mar. 2018. 2219-2840 |
url |
https://repositorio.ufrn.br/jspui/handle/123456789/25413 http://dx.doi.org/10.3748/wjg.v18.i31.4162 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Baishideng |
publisher.none.fl_str_mv |
Baishideng |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFRN instname:Universidade Federal do Rio Grande do Norte (UFRN) instacron:UFRN |
instname_str |
Universidade Federal do Rio Grande do Norte (UFRN) |
instacron_str |
UFRN |
institution |
UFRN |
reponame_str |
Repositório Institucional da UFRN |
collection |
Repositório Institucional da UFRN |
bitstream.url.fl_str_mv |
https://repositorio.ufrn.br/bitstream/123456789/25413/3/Growth%20inhibitory%20effects%20of%20Phyllanthus%20niruri_2012.pdf.txt https://repositorio.ufrn.br/bitstream/123456789/25413/4/Growth%20inhibitory%20effects%20of%20Phyllanthus%20niruri_2012.pdf.jpg https://repositorio.ufrn.br/bitstream/123456789/25413/1/GrowthInhibitoryEffects_Araujo_2012.pdf https://repositorio.ufrn.br/bitstream/123456789/25413/2/license.txt |
bitstream.checksum.fl_str_mv |
889d9ec68ce3b2dbb280f891872268a1 f389410f728a8fa280878e14ddd1f2e3 33a56cd3f7e51af7595f1d72d3de95a2 8a4605be74aa9ea9d79846c1fba20a33 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 MD5 |
repository.name.fl_str_mv |
Repositório Institucional da UFRN - Universidade Federal do Rio Grande do Norte (UFRN) |
repository.mail.fl_str_mv |
|
_version_ |
1802117578991075328 |